Silk fibroin nanoparticles dyeing indocyanine green for imaging-guided photo-thermal therapy of glioblastoma

doi:10.1080/10717544.2018.1428244

科研成果详情

题名	Silk fibroin nanoparticles dyeing indocyanine green for imaging-guided photo-thermal therapy of glioblastoma
作者	Xu, He-Lin1; ZhuGe, De-Li1; Chen, Pian-Pian 1; Tong, Meng-Qi1; Lin, Meng-Ting 1; Jiang, Xue 1; Zheng, Ya-Wen 1; Chen, Bin 2; Li, Xiao-Kun1; Zhao, Ying-Zheng1,3
发表日期	2018-01-25
发表期刊	DRUG DELIVERY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Silk fibroin nanoparticles indocyanine green phototherapy glioblastoma
其他关键词	TARGETED THERAPY ; IN-VITRO ; TUMOR ; DELIVERY ; CANCER ; LIPOSOMES ; POLYMER ; AGENT
摘要	Silk was easily dyed in traditional textile industry because of its strong affinity to many colorants. Herein, the biocompatible silk fibroin was firstly extracted from Bombyx mori silkworm cocoons. And SF nanoparticles (SFNPs) were prepared for dyeing indocyanine green (ICG) and construct a therapeutic nano-platform (ICG-SFNPs) for photo-thermal therapy of glioblastoma. ICG was easily encapsulated into SFNPs with a very high encapsulation efficiency reaching to 97.7 +/- 1.1%. ICG-SFNPs exhibited a spherical morphology with a mean particle size of 209.4 +/- 1.4nm and a negative zeta potential of -31.9mV, exhibiting a good stability in physiological medium. Moreover, ICG-SFNPs showed a slow release profile of ICG in vitro, and only 24.51 +/- 2.27% of the encapsulated ICG was released even at 72h. Meanwhile, ICG-SFNPs exhibited a more stable photo-thermal effect than free ICG after exposure to near-infrared irradiation. The temperature of ICG-SFNPs rapidly increased by 33.9 degrees C within 10min and maintained for a longer time. ICG-SFNPs were also easily internalized with C-6 tumor cells in vitro, and a strong red fluorescence of ICG was observed in cytoplasm for cellular imaging. In vivo imaging showed that ICG-SFNPs were effectively accumulated inside tumor site of C-6 glioma-bearing Xenograft nude mice through vein injection. Moreover, the temperature of tumor site was rapidly rising up to kill tumor cells after local NIR irradiation. After treatment, its growth was completely suppressed with the relative tumor volume of 0.55 +/- 033 while free ICG of 33.72 +/- 1.90. Overall, ICG-SFNPs may be an effective therapeutic means for intraoperative phototherapy and imaging.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81603036, 81571392]; Zhejiang Provincial Natural Science FoundationNatural Science Foundation of Zhejiang Province [LY17H180008]; key research and development program of Zhejiang province [2018C03013]; Zhejiang provincial program for the cultivation of high-level innovative health talents; 151 talent project of Zhejiang province; 551 talent project of Wenzhou; School Talent Start Fund of Wenzhou Medical University [QTJ15020]
出版者	TAYLOR & FRANCIS LTD
出版地	ABINGDON
ISSN	1071-7544
EISSN	1521-0464
卷号	25 期号:1 页码:364-375
DOI	10.1080/10717544.2018.1428244
页数	12
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000423540900002
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	29366360
SCOPUSEID	2-s2.0-85048716943
通讯作者地址	[Xu, He-Lin]Department of Pharmaceutics,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou City,China
Scopus学科分类	Pharmaceutical Science
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12471
专题	药学院（分析测试中心）附属第一医院_超声影像科第二临床医学院、附属第二医院、育英儿童医院_影像医学与核医学_超声科
通讯作者	Xu, He-Lin
作者单位	1.Department of Pharmaceutics,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou City,China; 2.Department of Ultrasonography,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou City,China; 3.Department of Pharmaceutics,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou City,325035,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Xu, He-Lin,ZhuGe, De-Li,Chen, Pian-Pian,et al. Silk fibroin nanoparticles dyeing indocyanine green for imaging-guided photo-thermal therapy of glioblastoma[J]. DRUG DELIVERY,2018,25(1):364-375.
APA	Xu, He-Lin., ZhuGe, De-Li., Chen, Pian-Pian., Tong, Meng-Qi., Lin, Meng-Ting., ... & Zhao, Ying-Zheng. (2018). Silk fibroin nanoparticles dyeing indocyanine green for imaging-guided photo-thermal therapy of glioblastoma. DRUG DELIVERY, 25(1), 364-375.
MLA	Xu, He-Lin,et al."Silk fibroin nanoparticles dyeing indocyanine green for imaging-guided photo-thermal therapy of glioblastoma".DRUG DELIVERY 25.1(2018):364-375.