The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-kappa B2 activation

doi:10.1038/s41419-017-0122-4

科研成果详情

题名	The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-kappa B2 activation
作者	Guo, Gangqiang1; Ye, Sisi 1; Xie, Shangdan 2; Ye, Lele 1; Lin, Cong 2; Yang, Min 2; Shi, Xinyu1; Wang, Fangyan3; Li, Baoqing4; Li, Ming 5; Chen, Chaosheng6; Zhang, Lifang 1; Zhang, Huidi6; Xue, Xiangyang1
发表日期	2018-01-24
发表期刊	CELL DEATH & DISEASE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	NF-KAPPA-B ; TRANSCRIPTION FACTORS ; GENE-EXPRESSION ; PROGENITOR CELLS ; DNA METHYLATION ; THP-1 CELLS ; INFECTION ; DIFFERENTIATION ; PATHWAY ; INHIBITION
摘要	It has been hypothesized that human cytomegalovirus (HCMV) infection, especially in monocyte and CD34 (+) myeloid cells, acts as a important regulator of immune system to promote inflammation in multiple autoimmune diseases. The aim of this study was to elucidate the HCMV gene expression profiles in the peripheral blood mononuclear cells (PBMCs) of SLE patients and demonstrate the effect and mechanism of viral gene associated with SLE in mono-macrophages functions. Using two RNA-Seq techniques in combination with RT-PCR, 11 viral genes mainly associated with latent HCMV infection were identified in the PBMCs of SLE patients. Among these viral genes, US31 with previously unknown function was highly expressed in the PBMCs of SLE patients compared to healthy controls. Analysis of function indicated that US31 expression could induce inflammation in monocyte and macrophage and stimulate macrophage differentiation toward an M1 macrophage phenotype. Screening via protein chips in combination with bioinformatic analysis and consequent detection of mono-macrophages function indicates that the direct interaction between US31 and NF-kappa B2 contributed the NF-kappa B2 activation. Consequent analysis indicated US31 directly interacted with NF-kappa B2, contribute to the polyubiquitination of the phosphorylated p100 and consequent activation of NF-kappa B2. Taken together, our data uncovered a previously unknown role of the HCMV protein US31 in inducing NF-kappa B-mediated mono-macrophage inflammation in the pathogenesis and development of SLE. Our findings provide a foundation for the continued investigation of novel therapeutic targets for SLE patients.
资助项目	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81001343, 81472308, 81672707]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [Y2100909, LY12H05003]; Zhejiang Science and Technology Bureau [2016C33214]; Wenzhou Municipal Science and Technology Bureau [Y20160018]
出版者	NATURE PUBLISHING GROUP
出版地	LONDON
ISSN	2041-4889
卷号	9 期号:2 页码:104
DOI	10.1038/s41419-017-0122-4
页数	15
WOS类目	Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:000427385100007
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29367719
PMC记录号	PMC5833803
SCOPUSEID	2-s2.0-85041076074
通讯作者地址	[Zhang, Huidi]Department of Nephrology,First Affiliated Hospital,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Immunology;Cellular and Molecular Neuroscience;Cell Biology;Cancer Research
TOP期刊	TOP期刊
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12119
专题	检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系附属第二医院附属第一医院第二临床医学院、附属第二医院、育英儿童医院附属第一医院_肾内科基础医学院（机能实验教学中心）_病原生物学与免疫学系基础医学院（机能实验教学中心）_病理学与病理生理学系基础医学院（机能实验教学中心）_基础医学研究中心_心脏再生医学研究所
通讯作者	Zhang, Huidi
作者单位	1.Department of Microbiology and Immunology,Institute of Molecular Virology and Immunology,Institute of Tropical Medicine,Wenzhou Medical University,Wenzhou,China; 2.Second Clinical College,Wenzhou Medical University,Wenzhou,China; 3.Department of Pathophysiology,Wenzhou Medical University,Wenzhou,China; 4.Department of Laboratory Medicine,Second Affiliated Hospital,Yuying Children's Hospital,Wenzhou Medical University,Wenzhou,China; 5.Cardiac Regeneration Research Institute,Wenzhou Medical University,Wenzhou,China; 6.Department of Nephrology,First Affiliated Hospital,Wenzhou Medical University,Wenzhou,China
第一作者单位	温州医科大学
通讯作者单位	附属第一医院; 肾内科
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Guo, Gangqiang,Ye, Sisi,Xie, Shangdan,et al. The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-kappa B2 activation[J]. CELL DEATH & DISEASE,2018,9(2):104.
APA	Guo, Gangqiang., Ye, Sisi., Xie, Shangdan., Ye, Lele., Lin, Cong., ... & Xue, Xiangyang. (2018). The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-kappa B2 activation. CELL DEATH & DISEASE, 9(2), 104.
MLA	Guo, Gangqiang,et al."The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-kappa B2 activation".CELL DEATH & DISEASE 9.2(2018):104.