Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages

doi:10.5114/aoms.2016.61909

科研成果详情

题名	Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages
作者	Liu, Fen 1; Wang, Yi 2; Xu, Jing3; Liu, Fangqiang 3; Hu, Rongdang2; Deng, Hui3
发表日期	2016-10
发表期刊	ARCHIVES OF MEDICAL SCIENCE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	periodontitis macrophages CD36 acetyl CoA acyltransferase 1 ATP-binding cassette G1
其他关键词	FOAM CELL-FORMATION ; PERIODONTAL-DISEASE ; HUMAN MONOCYTES ; ACYL-COENZYME ; CD36 ; LPS
摘要	Introduction: Cardiovascular diseases are positively correlated with periodontal disease. However, the molecular mechanisms linking atherosclerosis and periodontal infection are not clear. This study aimed to determine whether Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) altered the expression of genes regulating cholesterol metabolism in macrophages in the presence of low-density lipoprotein (LDL). Material and methods: THP-1-derived macrophages were exposed to different concentrations (0.1, 1, 10 mu g/ml) of LPS in the presence of 50 mu g/ml native LDL Macrophages were also incubated with 1 mu g/ml LPS for varying times (0, 24, 48, or 72 h) in the presence of native LDL. Foam cell formation was determined by oil red 0 staining and cholesterol content quantification. CD36, lectin-like oxidized LDL receptor-1 (LOX-1), ATP-binding cassette G1 (ABCG1), and acetyl CoA acyltransferase 1 (ACAT1) expression levels were measured by western blot and qRT-PCR. Results: Foam cell formation was induced in a time- and concentration-dependent manner as assessed by both morphological and biochemical criteria. Pg-LPS caused downregulation of CD36 and ABCG1 but upregulation of ACAT1, while LOX-1 expression was not affected (p = 0.137). Conclusions: Pg-LPS appears to be an important link in the development of atherosclerosis by mechanisms targeting cholesterol homeostasis, namely, excess cholesterol ester formation via ACAT1 and reduced cellular cholesterol efflux via ABCG1.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81200795]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY12H14003, LQ12H09003]
出版者	TERMEDIA PUBLISHING HOUSE LTD
出版地	POZNAN
ISSN	1734-1922
EISSN	1896-9151
卷号	12 期号:5 页码:959-967
DOI	10.5114/aoms.2016.61909
页数	9
WOS类目	Medicine, General & Internal
WOS研究方向	General & Internal Medicine
WOS记录号	WOS:000382410700006
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	27695485
PMC记录号	PMC5016584
SCOPUSEID	2-s2.0-84989859995
通讯作者地址	[Deng, Hui]Department of Periodontics,School of Stomatology,Wenzhou Medical University,No. 113 West Xueyuan Road,Wenzhou, Zhejiang,China
Scopus学科分类	Medicine (all)
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/11969
专题	基础医学院（机能实验教学中心）_形态学系_组织胚胎学口腔医学院、附属口腔医院_口腔正畸
通讯作者	Deng, Hui
作者单位	1.Department of Histology and Embryology,Wenzhou Medical University,Wenzhou, Zhejiang,China; 2.Department of Orthodontics,School of Stomatology,Wenzhou Medical University,No. 113 West Xueyuan Road,Wenzhou, Zhejiang,China; 3.Department of Periodontics,School of Stomatology,Wenzhou Medical University,No. 113 West Xueyuan Road,Wenzhou, Zhejiang,China
第一作者单位	组织胚胎学
通讯作者单位	口腔医学院、附属口腔医院
第一作者的第一单位	组织胚胎学
推荐引用方式 GB/T 7714	Liu, Fen,Wang, Yi,Xu, Jing,et al. Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages[J]. ARCHIVES OF MEDICAL SCIENCE,2016,12(5):959-967.
APA	Liu, Fen, Wang, Yi, Xu, Jing, Liu, Fangqiang, Hu, Rongdang, & Deng, Hui. (2016). Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages. ARCHIVES OF MEDICAL SCIENCE, 12(5), 959-967.
MLA	Liu, Fen,et al."Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages".ARCHIVES OF MEDICAL SCIENCE 12.5(2016):959-967.