Identification of methicillin-resistant Staphylococcus aureus ST8 isolates in China with potential high virulence

doi:10.1080/22221751.2022.2031310

科研成果详情

题名	Identification of methicillin-resistant Staphylococcus aureus ST8 isolates in China with potential high virulence
作者	Wang, Xinyi 1; Zhao, Huilin 1; Wang, Bingjie 1; Zhou, Ying 1; Xu, Yanlei 2; Rao, Lulin3; Ai, Wenxiu 3; Guo, Yinjuan 1; Wu, Xiaocui 1; Yu, Jingyi 1; Hu, Longhua 4; Han, Lizhong 5; Chen, Shuying3; Chen, Liang 6,7; Yu, Fangyou 1
发表日期	2022-12-31
发表期刊	EMERGING MICROBES & INFECTIONS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Methicillin-resistant Staphylococcus aureus ST8 SCCmec cassette virulence pathogenicity
其他关键词	MOLECULAR EPIDEMIOLOGY ; SYSTEM ; CLONES ; SAERS
摘要	Methicillin-resistant Staphylococcus aureus (MRSA) ST8 strains have spread worldwide, causing outbreaks in various regions. However, this clone has only been sporadically reported in China. Consequently, detailed information regarding the phylogeny and potential virulence of S. aureus ST8 strains in China remains unknown. In this study, we characterized six ST8 strains collected from three tertiary hospitals in China, including three MRSA (MR50, MR526, and MR254) and three MSSA (H78, H849 and H863). Whole genome sequencing and phylogenetic analysis showed that the six strains formed two separate clusters, including two (MR50 and MR526) and four (MR254, H78, H849 and H863) isolates, respectively. Among them, MR50 and MR526 harboured spa t008, SCCmec IVa, arginine catabolic mobile element, and were phylogenetically close to the epidemic USA300 strains, while other four strains belonged to spa t9101 and formed a unique branch. MR254 carried a novel hybrid SCCmec element (namely SCCmec254). Same as the USA300 prototype strain LAC, the China S. aureus ST8 strains produced weak biofilms except MR254. Among them, MR254 had significantly stronger haemolysis ability and higher alpha-toxin levels than others, while MR526 showed comparable haemolysis and alpha-toxin production levels as USA300-LAC. In mouse skin abscess model, MR254 showed particularly strong invasions, accompanied by necrosis, while MR526 exhibited similar infection levels as USA300-LAC. These data suggested that the China MRSA ST8 isolates (e.g. MR254 and MR526) were highly virulent, displaying higher or similar virulence potential as the epidemic USA300 strain. Active surveillance should be enacted to closely monitor the further spread of these hyper-virulent MRSA strains in China.
资助项目	Natural Science Fund of ChinaNational Natural Science Foundation of China (NSFC) [82072343]
出版者	TAYLOR & FRANCIS LTD
出版地	ABINGDON
ISSN	2222-1751
EISSN	2222-1751
卷号	11 期号:1 页码:507-518
DOI	10.1080/22221751.2022.2031310
页数	12
WOS类目	Immunology ; Infectious Diseases ; Microbiology
WOS研究方向	Immunology ; Infectious Diseases ; Microbiology
WOS记录号	WOS:000754229500001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	35044290
PMC记录号	PMC8843119
SCOPUSEID	2-s2.0-85124498627
通讯作者地址	[Yu, Fangyou]Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,200433,China
Scopus学科分类	Epidemiology;Parasitology;Microbiology;Immunology;Drug Discovery;Infectious Diseases;Virology
TOP期刊	TOP期刊
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/11335
专题	检验医学院（生命科学学院、生物学实验教学中心）第一临床医学院（信息与工程学院）、附属第一医院附属第一医院
通讯作者	Yu, Fangyou
作者单位	1.Department of Clinical Laboratory,Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,China; 2.Jiangxi Provincial Key Laboratory of Preventive Medicine,School of Public Health,Nanchang University,Nanchang,China; 3.Department of Laboratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 4.Department of Jiangxi Provincial Key Laboratory of Medicine,Clinical Laboratory of the Second Affiliated Hospital of Nanchang University,Nanchang,China; 5.Department of Laboratory Medicine,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China; 6.Center for Discovery and Innovation,Hackensack Meridian Health,Nutley,United States; 7.Department of Medical Sciences,Hackensack Meridian School of Medicine,Nutley,United States
推荐引用方式 GB/T 7714	Wang, Xinyi,Zhao, Huilin,Wang, Bingjie,et al. Identification of methicillin-resistant Staphylococcus aureus ST8 isolates in China with potential high virulence[J]. EMERGING MICROBES & INFECTIONS,2022,11(1):507-518.
APA	Wang, Xinyi., Zhao, Huilin., Wang, Bingjie., Zhou, Ying., Xu, Yanlei., ... & Yu, Fangyou. (2022). Identification of methicillin-resistant Staphylococcus aureus ST8 isolates in China with potential high virulence. EMERGING MICROBES & INFECTIONS, 11(1), 507-518.
MLA	Wang, Xinyi,et al."Identification of methicillin-resistant Staphylococcus aureus ST8 isolates in China with potential high virulence".EMERGING MICROBES & INFECTIONS 11.1(2022):507-518.