Aberrantly Upregulated EF4 Is Crucial for The Proliferation and Migration of Bladder Urothelial Carcinoma Cells via Orchestration of Mitochondrial Oxidative Phosphorylation

doi:10.16476/j.pibb.2020.0231

科研成果详情

题名	Aberrantly Upregulated EF4 Is Crucial for The Proliferation and Migration of Bladder Urothelial Carcinoma Cells via Orchestration of Mitochondrial Oxidative Phosphorylation
其他题名	EF4影响线粒体氧化磷酸化并调控膀胱癌细胞增殖及迁移
作者	Hua Chao-Ju 1,6; Dai Fei 1,6; Deng Yao 2,3,4; Lu Jun-Wan 2,3,4; Zhou Ning-Ning 2,3,4; Li Wan-Qi 5; Zhang Ye 1,6; Huang Ka-Te2,3,4; Liu Yong-Zhang2,3,4; Lu Bin 2,3,4; Wei Tao-Tao 1,6
发表日期	2021-03
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	EF4 tumorigenesis mitochondrial translation mitochondrial oxidative phosphorylation bladder urothelial carcinoma
其他关键词	ELONGATION-FACTOR ; PROTEIN-SYNTHESIS ; ESCHERICHIA-COLI ; SIGNAL PEPTIDASE ; CANCER-CELLS ; METABOLISM ; LEPA ; IDENTIFICATION ; TRANSLATION ; BIOGENESIS
摘要	Elongation factor 4 (EF4) is a non-conventional elongation factor which regulates protein synthesis in mitochondria. In this study, we explored its function in bladder urothelial carcinoma. By analyzing the expression of EF4 in bladder urothelial carcinoma and adjacent normal tissues, we found that EF4 was aberrantly elevated in multiple cohorts of bladder cancer patients. Notably, the upregulation of EF4 was positively associated with tumor progression. By manipulating EF4 expression in HTB-9 and T-24 bladder cancer cells, the effects of upregulated EF4 was investigated. Knockdown of EF4 suppressed the proliferation and colony formation in bladder cancer cells; the ability of cells to migrate in vitro was also retarded. Knockdown of EF4 down-regulated the expression of mitochondrial DNA-encoded subunits of electron transfer chain complexes, and resulted in the dysfunction of mitochondrial oxidative phosphorylation. These results define a tumor-supportive role for EF4 by maintaining the protein synthesis within the mitochondria, which may serve as a potential therapeutic target in bladder urothelial carcinoma.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [92054108, 91954101, 31771534, 31570772]
出版者	CHINESE ACAD SCIENCES, INST BIOPHYSICS
出版地	BEIJING
ISSN	1000-3282
卷号	48 期号:3 页码:317-327
DOI	10.16476/j.pibb.2020.0231
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000638231700008
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85105926643
通讯作者地址	[Lü, Bin]Protein Quality Control and Diseases Laboratory,Key Laboratory of Medical Genetics of Zhejiang Province,Key Laboratory of Laboratory Medicine,Ministry of Education of China,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,325035,China ; [Wei, Tao-Tao]National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing,100101,China
Scopus学科分类	Biophysics;Biochemistry
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/11081
专题	附属第二医院检验医学院（生命科学学院、生物学实验教学中心）附属第一医院附属第一医院_病理科眼视光学院（生物医学工程学院）、附属眼视光医院检验医学院（生命科学学院、生物学实验教学中心）_细胞生物学与遗传学系
通讯作者	Lu Bin; Wei Tao-Tao
作者单位	1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China; 2.Wenzhou Med Univ, Prot Qual Control & Dis Lab, Key Lab Med Genet Zhejiang Prov, Wenzhou 325035, Peoples R China; 3.Wenzhou Med Univ, Minist Educ China, Key Lab Lab Med, Wenzhou 325035, Peoples R China; 4.Wenzhou Med Univ, Sch Lab Med & Life Sci, Wenzhou 325035, Peoples R China; 5.Peking Univ, Yuanpei Coll, Beijing 100871, Peoples R China; 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Hua Chao-Ju,Dai Fei,Deng Yao,et al. Aberrantly Upregulated EF4 Is Crucial for The Proliferation and Migration of Bladder Urothelial Carcinoma Cells via Orchestration of Mitochondrial Oxidative Phosphorylation[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2021,48(3):317-327.
APA	Hua Chao-Ju., Dai Fei., Deng Yao., Lu Jun-Wan., Zhou Ning-Ning., ... & Wei Tao-Tao. (2021). Aberrantly Upregulated EF4 Is Crucial for The Proliferation and Migration of Bladder Urothelial Carcinoma Cells via Orchestration of Mitochondrial Oxidative Phosphorylation. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 48(3), 317-327.
MLA	Hua Chao-Ju,et al."Aberrantly Upregulated EF4 Is Crucial for The Proliferation and Migration of Bladder Urothelial Carcinoma Cells via Orchestration of Mitochondrial Oxidative Phosphorylation".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 48.3(2021):317-327.

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