Colorectal cancer cell-derived extracellular vesicles transfer miR-221-3p to promote endothelial cell angiogenesis via targeting suppressor of cytokine signaling 3

doi:10.1016/j.lfs.2021.119937

科研成果详情

题名	Colorectal cancer cell-derived extracellular vesicles transfer miR-221-3p to promote endothelial cell angiogenesis via targeting suppressor of cytokine signaling 3
作者	Dokhanchi, Maryam 1; Pakravan, Katayoon 2; Zareian, Sara 1; Hussen, Bashdar Mahmud 3; Farid, Mahsa 1; Razmara, Ehsan 4; Mossahebi-Mohammadi, Majid 5; Cho, William C.6; Babashah, Sadegh 2
发表日期	2021-11-15
发表期刊	LIFE SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Colorectal cancer Diagnosis Extracellular vesicles Angiogenesis miRNA Suppressor of cytokine signaling 3
其他关键词	MICRORNAS ; ROLES ; PCR
摘要	Background: Secreted microRNAs (miRNAs) can serve as promising diagnostic markers for colorectal cancer (CRC). Herein, we evaluated the potential clinical significance of a signature of four circulating serum-derived miRNAs in CRC. We also demonstrated that extracellular vesicles (EVs) containing miR-221-3p could facilitate endothelial cell angiogenesis. Methods: The expressions of four circulating serum-derived miRNAs (miR-19a-3p, miR-203-3p, miR-221-3p, and let-7f-5p) were measured by real-time quantitative PCR, and their associations with lymph node metastasis were determined in CRC patients. Receiver operating characteristic curve analysis was used to determine their diagnostic accuracy. EVs were isolated and characterized from the conditioned media of human CRC cells (HCT116 and Caco2). Cell proliferation, transwell migration, and tube formation assays were performed to investigate the pro-angiogenic effect of miR-221-3p transferred by CRC-EVs into the endothelial cells. In silico analysis was used to show the regulatory functions of miR-221-3p on SOCS3, validated by luciferase and Western blotting assays. Results: The expression levels of serum-derived miR-19a-3p, miR-203-3p, miR-221-3p, and let-7f-5p were significantly higher in CRC than in healthy individuals. The expression of miR-19a-3p, miR-203-3p, and miR221-3p were positively correlated with the lymph node metastasis status. Moreover, SOCS3 was identified as a direct target of miR-221-3p and the secreted miR-221-3p shuttled by CRC-EVs regulated STAT3/VEGFR-2 signaling axis by targeting SOCS3 in endothelial cells. CRC-EVs promoted endothelial cell proliferation, migration, and the formation of vessel-like structures. The proangiogenic effect of CRC-EVs on the cells was recapitulated by miR-221-3p overexpression, showing the importance of EVs-derived miR-221-3p in promoting endothelial cell angiogenesis. Conclusion: We introduced a signature of four-circulating miRNAs (miR-19a-3p, miR-203-3p, miR-221-3p, and let-7f-5p) as a novel diagnostic biomarker for CRC. Besides, we revealed that miR-221-3p induces endothelial cell angiogenesis in vitro by targeting SOCS3.
出版者	PERGAMON-ELSEVIER SCIENCE LTD
出版地	OXFORD
ISSN	0024-3205
EISSN	1879-0631
卷号	285 页码:119937
DOI	10.1016/j.lfs.2021.119937
页数	11
WOS类目	Medicine, Research & Experimental ; Pharmacology & Pharmacy
WOS研究方向	Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000703198800012
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	34508764
SCOPUSEID	2-s2.0-85114993606
通讯作者地址	[Cho, William C.]Department of Clinical Oncology,Queen Elizabeth Hospital,Kowloon,Hong Kong ; [Babashah, Sadegh]Department of Molecular Genetics,Faculty of Biological Sciences,Tarbiat Modares University,P.O. Box: 14115-154,Tehran,Iran
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all);Pharmacology, Toxicology and Pharmaceutics (all)
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/10636
专题	药学院（分析测试中心）卓越中心_国际生长因子研究院
通讯作者	Cho, William C.; Babashah, Sadegh
作者单位	1.Department of Biology,Faculty of Sciences,Islamic Azad University,Tehran,Iran; 2.Department of Molecular Genetics,Faculty of Biological Sciences,Tarbiat Modares University,Tehran,Iran; 3.Department of Pharmacognosy,College of Pharmacy,Hawler Medical University,Kurdistan Region,Iraq; 4.Department of Medical Genetics,School of Medical Sciences,Tarbiat Modares University,Tehran,Iran; 5.School of Pharmaceutical Sciences and International Collaborative Center on Growth Factor Research,Wenzhou Medical University,Wenzhou,China; 6.Department of Clinical Oncology,Queen Elizabeth Hospital,Kowloon,Hong Kong
推荐引用方式 GB/T 7714	Dokhanchi, Maryam,Pakravan, Katayoon,Zareian, Sara,et al. Colorectal cancer cell-derived extracellular vesicles transfer miR-221-3p to promote endothelial cell angiogenesis via targeting suppressor of cytokine signaling 3[J]. LIFE SCIENCES,2021,285:119937.
APA	Dokhanchi, Maryam., Pakravan, Katayoon., Zareian, Sara., Hussen, Bashdar Mahmud., Farid, Mahsa., ... & Babashah, Sadegh. (2021). Colorectal cancer cell-derived extracellular vesicles transfer miR-221-3p to promote endothelial cell angiogenesis via targeting suppressor of cytokine signaling 3. LIFE SCIENCES, 285, 119937.
MLA	Dokhanchi, Maryam,et al."Colorectal cancer cell-derived extracellular vesicles transfer miR-221-3p to promote endothelial cell angiogenesis via targeting suppressor of cytokine signaling 3".LIFE SCIENCES 285(2021):119937.