Knock-Down of HOXB8 Prohibits Proliferation and Migration of Colorectal Cancer Cells via Wnt/beta-Catenin Signaling Pathway

doi:10.12659/MSM.912218

科研成果详情

题名	Knock-Down of HOXB8 Prohibits Proliferation and Migration of Colorectal Cancer Cells via Wnt/beta-Catenin Signaling Pathway
作者	Li, Xiang1; Lin, Han 2; Jiang, Feizhao3; Lou, Yongliang1; Ji, Ling3; Li, Shaotang3
发表日期	2019-01-24
发表期刊	MEDICAL SCIENCE MONITOR 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Cell Proliferation Colorectal Neoplasms Wnt Signaling Pathway
其他关键词	C-MYC ; MESENCHYMAL TRANSITION ; APC DEFICIENCY ; BETA-CATENIN ; EXPRESSION ; GENES ; BAX ; IDENTIFICATION ; INHIBITION ; PHENOTYPES
摘要	Background: There has been no research on the mechanism of HOXB8 action on colorectal cancer so far. This study was designed to investigate the mechanism of HOXB8 regulating colorectal cancer cell proliferation and invasion in vivo and in vitro. Material/Methods: HOXB8 shRNA, HOXB8 overexpression, and negative control vector were designed and stably transfected into HCT116 cells. MTT assays were performed to detect cell proliferation. Western blot was utilized to detect HOXB8 expression level in HCT116 stable cells. The invasive and migration abilities were detected by Transwell assay and wound-healing assay. Results: HOXB8 knockdown inhibited cell proliferation. The invasiveness of HCT116 cells was significantly reduced following HOXB8 depletion compared with that in the shRNA control group, whereby the rates were reduced by 67% in HOXB8 knockdown group. The wound-healing rate of HOXB8 over-expression cells was significantly increased comparing with that of cells in the blank control group (P<0.05). HOXB8 knockdown promotes apoptosis of HCT116 cells. The expression of E-cadherin was restrained in the HOXB8 over-expression group and increased in the HOXB8 knockdown group. Conclusions: Knock-down of HOXB8 prohibits the proliferation and migration of colorectal cancer cells via the Wnt/beta-catenin signaling pathway and the downregulation of various factors, such as MMP2, c-Myc, CyclinD1, and vimentin. Our data suggested that HOXB8 has great potential to be developed as a novel therapeutic agent for the treatment of human colorectal cancer.
资助项目	Key Discipline of Zhejiang Province in Medical Technology (First Class, Category A); funds of Zhejiang experimental animal science and technology project [2015C37103]; Scientific Research Fund of Zhejiang Provincial Education Department [Y201326738]
出版者	INT SCIENTIFIC INFORMATION, INC
出版地	MELVILLE
ISSN	1643-3750
EISSN	1643-3750
卷号	25 页码:711-720
DOI	10.12659/MSM.912218
页数	10
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:000456690200003
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	30677006
PMC记录号	PMC6357822
SCOPUSEID	2-s2.0-85060555391
通讯作者地址	[Li, Shaotang]Department of Colorectal Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Medicine (all)
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1052
专题	第一临床医学院（信息与工程学院）、附属第一医院_中心实验室检验医学院（生命科学学院、生物学实验教学中心）附属第一医院_肛肠外科
通讯作者	Li, Shaotang
作者单位	1.Zhejiang Provincial Key Laboratory of Medical Genetics,Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China; 2.Central Laboratory,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.Department of Colorectal Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Li, Xiang,Lin, Han,Jiang, Feizhao,et al. Knock-Down of HOXB8 Prohibits Proliferation and Migration of Colorectal Cancer Cells via Wnt/beta-Catenin Signaling Pathway[J]. MEDICAL SCIENCE MONITOR,2019,25:711-720.
APA	Li, Xiang, Lin, Han, Jiang, Feizhao, Lou, Yongliang, Ji, Ling, & Li, Shaotang. (2019). Knock-Down of HOXB8 Prohibits Proliferation and Migration of Colorectal Cancer Cells via Wnt/beta-Catenin Signaling Pathway. MEDICAL SCIENCE MONITOR, 25, 711-720.
MLA	Li, Xiang,et al."Knock-Down of HOXB8 Prohibits Proliferation and Migration of Colorectal Cancer Cells via Wnt/beta-Catenin Signaling Pathway".MEDICAL SCIENCE MONITOR 25(2019):711-720.