Photodynamic Treatment of Colorectal Cancer Using Chlorin e6-Loaded Poly(lactide-co-glycolide)-Based Nanoparticles

doi:10.1166/jbn.2021.3170

科研成果详情

题名	Photodynamic Treatment of Colorectal Cancer Using Chlorin e6-Loaded Poly(lactide-co-glycolide)-Based Nanoparticles
作者	Lin, Beibei1,2; Xu, Xuegu1,2; Zhang, Xiaobi 1,2; Yu, Yinfei1,2; Wang, Xiaoling1,2
发表日期	2021-10
发表期刊	JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Photodynamic Therapy Chlorin e6 (Ce6) Poly(lactide-co-glycolide) (PLGA) Cancer Therapy Targeted Delivery
其他关键词	MESENCHYMAL STEM-CELLS ; MEDIATED ENDOCYTOSIS ; CELLULAR UPTAKE ; GENE-TRANSFER ; IN-VITRO ; THERAPY ; DELIVERY ; PLGA ; BIODISTRIBUTION ; MEMBRANE
摘要	We prepared poly(lactide-co-glycolide) (PLGA) encapsulated with chlorin e6 (Ce6) in an effort to increase the stability and efficiency of photosensitizers for photodynamic therapy (PDT). We determined that Ce6-loaded PLGA nanoparticles (PLGA-Ce6 NPs) had drug-loading efficiency of 5%. The efficiency of encapsulation was 82%, the zeta potential was25 mV, and the average diameter was 130 nm. The encapsulation of Ce6 in PLGA nanoparticles showed excellent stability. The nanoparticles exhibited sustained Ce6 release profiles with 50% released at the end of 3 days, whereas free Ce6 showed rapid release within 1 day. Ce6 release patterns were controlled by encapsulation into PLGA. The uptake of PLGA-Ce6 NPs was significantly enhanced by endocytosis in the first 8 hours in the HCT-116 cell line. An intracellular reactive oxygen species assay revealed the enhanced uptake of the nanoparticles. An in vitro anti-tumor activity assay showed that the PLGA-Ce6 NPs exhibited enhanced phototoxicity toward HCT-116 cells and a slightly lower IC50 value in HCT-116 cells than Ce6 solution alone. Exposure of HCT-116 cell spheroids to PLGA-Ce6 NPs penetrated more profoundly and had better phototoxicity than pure drugs. These findings suggest that PLGA-Ce6 NPs might serve as PDT for colorectal cancer.
资助项目	Wenzhou City Science and Technology Project of Zhejiang Province of China [Y20210216, Y2020182]; Special Scientific Research Funding for Pharmacy of Zhejiang Pharmaceutical Association Hospital [2020ZYY40]
出版者	AMER SCIENTIFIC PUBLISHERS
出版地	VALENCIA
ISSN	1550-7033
EISSN	1550-7041
卷号	17 期号:10 页码:1939-1950
DOI	10.1166/jbn.2021.3170
页数	12
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Biomaterials
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS记录号	WOS:000712815000001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	34706794
SCOPUSEID	2-s2.0-85121190986
通讯作者地址	[Wang, Xiaoling]Wenzhou Med Univ, Hosp Eye, Dept Pharm, Wenzhou 325000, Peoples R China. ; [Wang, Xiaoling]Wenzhou Med Univ, Sch Ophthalmol & Optometry, Dept Pharm, Wenzhou 325000, Peoples R China.
Scopus学科分类	Medicine (all)
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/999
专题	眼视光学院（生物医学工程学院）、附属眼视光医院_药剂科
通讯作者	Wang, Xiaoling
作者单位	1.Wenzhou Med Univ, Hosp Eye, Dept Pharm, Wenzhou 325000, Peoples R China; 2.Wenzhou Med Univ, Sch Ophthalmol & Optometry, Dept Pharm, Wenzhou 325000, Peoples R China
第一作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院_药剂科
通讯作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院_药剂科
第一作者的第一单位	眼视光学院（生物医学工程学院）、附属眼视光医院_药剂科
推荐引用方式 GB/T 7714	Lin, Beibei,Xu, Xuegu,Zhang, Xiaobi,et al. Photodynamic Treatment of Colorectal Cancer Using Chlorin e6-Loaded Poly(lactide-co-glycolide)-Based Nanoparticles[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2021,17(10):1939-1950.
APA	Lin, Beibei, Xu, Xuegu, Zhang, Xiaobi, Yu, Yinfei, & Wang, Xiaoling. (2021). Photodynamic Treatment of Colorectal Cancer Using Chlorin e6-Loaded Poly(lactide-co-glycolide)-Based Nanoparticles. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 17(10), 1939-1950.
MLA	Lin, Beibei,et al."Photodynamic Treatment of Colorectal Cancer Using Chlorin e6-Loaded Poly(lactide-co-glycolide)-Based Nanoparticles".JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 17.10(2021):1939-1950.