姜黄素对急性百草枯中毒大鼠肝损伤中炎症反应的影响和机制

doi:10.3969/j.issn.1002-1949.2015.01.014

科研成果详情

题名	姜黄素对急性百草枯中毒大鼠肝损伤中炎症反应的影响和机制
其他题名	Anti - inflammatory effect of curcumin on paraquat - induced liver injury in rats
作者	韩文文; 支绍册; 赵倩; 赵光举; 洪广亮; 李萌芳; 吴斌; 邱俏檬; 卢中秋
发表日期	2015
发表期刊	中国急救医学影响因子和分区
语种	中文
原始文献类型	期刊论文
关键词	百草枯肝损伤姜黄素核因子-κB(NF-κB) 炎症
其他关键词	Paraquat ; Liver injury ; Curcumin ; Nuclear factor-κB (NF-κB) ; Inflammation
摘要	目的探讨姜黄素(Curcumin, CU)对急性百草枯(Paraquat, PQ)中毒大鼠肝损伤中炎症反应的影响和机制。方法将60只清洁级SD大鼠随机分为4组:正常对照组(NS组,n = 15),姜黄素对照组(CU组,n = 15),百草枯中毒组(PQ组,n=15),姜黄素干预组(PQ + CU组,n = 15)。各组大鼠处理后第1、3、7天麻醉处死,并留取肝组织和血标本。光镜下观察各组肝组织病理变化情况,化学比色法检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBIL)水平的变化,逆转录-聚合酶链式反应(RT-PCR)法检测转录因子TNF-α、IL-1β mRNA表达情况,双抗夹心酶联免疫吸附法(ELISA)检测TNF-α、IL-1β蛋白表达情况,Western blot检测NF-kB蛋白表达情况。结果光镜下,PQ组大鼠肝组织损伤严重,PQ +CU组大鼠肝组织损伤程度较PQ组有所减轻。CU组与NS组各测定指标比较差异无统计学意义(P>0.05)。与NS组比较,PQ组ALT、AST、TBIL水平明显升高,ALT、AST在第3天达到峰值,TBIL在第1、3、7天持续升高(均P<0.05);与PQ组比较,PQ + CU组在第1、3、7天ALT、AST、TBIL水平明显降低(均P<0.05)。与NS组比较,PQ组在第1、3、7天TNF-α mRNA、IL-1β mRNA和蛋白表达量明显升高(均P<0.05);与PQ组比较,PQ + CU组TNF-α mRNA、IL-1β mRNA表达量明显降低(P<0. 05),PQ + CU组肝组织TNF-α、IL-1 β明显降低(P<0. 05)。与NS组比较,PQ组在第1、3、7天NF-KB蛋白表达量明显升高(均P<0. 05);与PQ组比较,PQ + CU组在第1、3、7天NF-KB 蛋白表达量明显降低(均P<0.05)。结论炎症反应是参与急性百草枯中毒肝损伤的重要机制之一,姜黄素通过抑制肝组织NF-kB炎症通路活化,使下游炎症因子TNF-αIL-1β水平降低,减轻百草枯中毒大鼠肝损伤的炎症反应,对急性百草枯中毒大鼠肝损伤有一定的保护作用。
其他摘要	Objective To investigate the anti - inflammatory effect of curcumin on paraquat-induced liver injury in rats and the underlying mechanisms. Methods Sixty Sprague - Dawley male rats of clean grade were randomly divided into four groups: the normal control group (NS group, n = 15), curcumin control group (CU group, n = 15),paraquat poisoning group (PQ group, n = 15),curcumin intervention group (PQ + CU group, n = 15). After PQ or NS administration, all rats were anesthetized and sacrificed on days 1, 3,7,and their liver tissues and blood samples were collected. Pathological changes of liver tissues were observed by light microscopy. We assessed the level of alanine aminotransferase (ALT), aspartate transaminase (AST),total bilirubin (TBIL) in the serum by chemical colorimetric method. The expression of tumor necrosis factor - (TNF - α) mRNA, interleukin - 1(IL-1β) mRNA was measured by reverse transcription - polymerase chain (RT -PCR),and the expression of TNF - α, IL - 1β protein in the liver tissue was measured by double antibody sandwich enzyme - linked immunosorbent assay (ELISA). The expression of NF - kB protein was measured by Western blotting. Results In comparison with NS group,the liver tissues of PQ group were damaged more severe, while histopathological lesions observed in PQ + CU group were milder significantly. There were no significant changes when compared with NS group and CU group (P > 0. 05). The levels of ALT,AST,TBIL in the serum in PQ group were dramatically higher than those in NS group on days 1,3,7 (P <0.05), and ALT, AST levels reached peak on the third day. Compared with the PQ group,the levels of ALT,AST,TBIL in the serum in PQ + CU group on days 1, 3 and 7 were obviously decreased (P <0. 05). Compared with the NS group, the expression of TNF - α, IL -1 β mRNA and protein,and the expression of NF - kB protein,were significantly increased in PQ group on days 1, 3, 7 (all P < 0. 05). The liver TNF - α level (30. 23 ± 1.90,43. 90 ±2. 83,39. 55 ± 0. 90) and IL - 1β level (40.48 ±3.32,85.06 ±2.76,47.1 ± l. 62) in PQ + CU group on days 1,3,7 were much lower than those in PQ group (TNF -α: 40. 77 ±2. 14,65. 02 ±2. 52, 52. 71 ± 1. 20;IL -1β: 64.84 ±1.95,112.95 ±2.97,73.69 ±2.12) (all P <0.05). Compared with the PQ group,the expression of TNF - α, IL - 1 β mRNA and NF - kB protein in PQ + CU group was decreased on days 1,3,7(all P <0.05). Conclusion Inflammation plays an important role in paraquat - induced acute liver damage. The beneficial effect of curcumin on paraquat - induced liver inflammation by the inhibition of NF - kB inflammation pathway, and then decrease the levels of TNF - a, IL - 1 β.
资助项目	浙江省“十二五”浙江省高校重点学科 ; 浙江省医学创新学科建设计划 ; 浙江省中医药重点学科计划
ISSN	1002-1949
卷号	35 期号:1 页码:62-67
DOI	10.3969/j.issn.1002-1949.2015.01.014
页数	7
收录类别	CSCD ; 万方 ; CNKI ; PKU ; 北大核心 ; ISTIC
学科领域	医药、卫生 ; 预防医学、卫生学
URL	查看原文
CSCD记录号	CSCD:5347005
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/99454
专题	第一临床医学院（信息与工程学院）、附属第一医院_急诊医学
作者单位	温州医科大学第一附属医院急诊医学中心, 浙江, 温州, 325000
第一作者单位	急诊医学
第一作者的第一单位	急诊医学
推荐引用方式 GB/T 7714	韩文文,支绍册,赵倩,等. 姜黄素对急性百草枯中毒大鼠肝损伤中炎症反应的影响和机制[J]. 中国急救医学,2015,35(1):62-67.
APA	韩文文., 支绍册., 赵倩., 赵光举., 洪广亮., ... & 卢中秋. (2015). 姜黄素对急性百草枯中毒大鼠肝损伤中炎症反应的影响和机制. 中国急救医学, 35(1), 62-67.
MLA	韩文文,et al."姜黄素对急性百草枯中毒大鼠肝损伤中炎症反应的影响和机制".中国急救医学 35.1(2015):62-67.