三七总皂苷对低氧大鼠肺动脉压和肺组织p38MAPK表达的影响

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题名	三七总皂苷对低氧大鼠肺动脉压和肺组织p38MAPK表达的影响
其他题名	Effect of Panax notoginoside on pulmonary arterial pressure and expression of p38 MAPK in lung tissues of hypoxic rats
作者	梁瑛琦1; 贾旭广 2; 石璐 2; 吴成云3; 王万铁2
发表日期	2010
发表期刊	中国病理生理杂志影响因子和分区
语种	中文
原始文献类型	期刊论文
关键词	三七总皂苷肺动脉高压低氧 p38MAP激酶
摘要	目的:探讨三七总皂苷(PNS)对低氧大鼠p38丝裂原活化蛋白激酶(p38 MAPK)表达的影响,及预防低氧性肺动脉高压(HPH)的作用和机制. 方法:将30只SD大鼠随机分为3组:正常对照组、低氧组和低氧 +PNS组. 观察各组大鼠平均肺动脉压(mPAP)、平均颈动脉压(mCAP)和右心室/(左心室+室间隔重量)比[RV/(LV+S)],免疫组化法和RT-PCR法分别检测肺小血管壁磷酸化p38MAPK(p-p38MAPK)蛋白和肺组织中mRNA的含量. 结果:与对照组相比,低氧组大鼠mPAP、RV/(LV+S)明显升高,肺小动脉p-p38 MAPK及肺组织p38MAPK mRNA含量显著升高(P<0.05). 低氧+PNS组mPAP、RV/(LV+S)、肺小动脉p-p38 MAPK及肺组织p38MAPK mRNA含量明显低于低氧组(P<0.05). 结论:PNS具有显著预防HPH的作用,其机制可能与其降低p38MAPK mRNA的表达有关
其他摘要	AIM: To observe the effect of Panax notoginoside (PNS) on the pulmonary artery pressure and the p38 mitogen - activated protein kinase(p38 MAPK) in lung tissues of rats treated with hypoxia. METHODS: Thirty adult male SD rats were randomly divided into 3 groups. The rats in normal control group were exposed to normal conditions, the rats in hypoxia group were exposed to isobaric hypoxia, and the rats in hypoxia + PNS group were treated with PNS under the condition of hypoxia. After 4 weeks of treatment, the mean pulmonary arterial pressure (mPAP) and the mean carotid arterial pressure (mCAP) were measured by cardiac catheterization. The heart was isolated, and the right ventricle (RV), left ventricle plus ventricular septum (LV + S) were' weighed to calculate the ratio of RV/(LV + S). The quantity of phospho - p38 MAPK(p - p38 MAPK) in rat pulmonary arterioles was determined by the method of immunohistochemistry and the mRNA content of p38 MAPK was tested by RT - PCR. RESULTS: The mPAP and RV/(LV + S) in hypoxia group were higher than those in normal control group. The expression of p - p38 MAPK in rat pulmonary arterioles and p38 MAPK mRNA in the lung tissues were higher than those in normal control group (P < 0. 05). The mPAP, RV/(LV + S), the expression of p - p38 MAPK in rat pulmonary arterioles and p38 MAPK mRNA in the lung tissues in hypoxia + PNS group were significantly lower than those in hypoxia group (P <0. 05). CONCLUSION: PNS possesses the preventive and therapeutic effect on hypoxic pulmonary hypertension by decreasing p - p38 MAPK and down - regulation of p38 MAPK mRNA in the lungs
资助项目	浙江省科技攻关项目
ISSN	1000-4718
卷号	26 期号:12 页码:2438-2441
收录类别	CSCD
学科领域	医药、卫生 ; 基础医学
CSCD记录号	CSCD:4090105
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/98994
专题	教务处附属第二医院基础医学院（机能实验教学中心）_病理学与病理生理学系_病理生理学
作者单位	1.温州医学院教务处, 浙江, 温州, 325035 ; 2.温州医学院病理生理学教研室, 浙江, 温州, 325035 ; 3.温州医学院附属第二医院呼吸内科, 浙江, 温州, 325035
第一作者单位	教务处
第一作者的第一单位	教务处
推荐引用方式 GB/T 7714	梁瑛琦,贾旭广,石璐,等. 三七总皂苷对低氧大鼠肺动脉压和肺组织p38MAPK表达的影响[J]. 中国病理生理杂志,2010,26(12):2438-2441.
APA	梁瑛琦, 贾旭广, 石璐, 吴成云, & 王万铁. (2010). 三七总皂苷对低氧大鼠肺动脉压和肺组织p38MAPK表达的影响. 中国病理生理杂志, 26(12), 2438-2441.
MLA	梁瑛琦,et al."三七总皂苷对低氧大鼠肺动脉压和肺组织p38MAPK表达的影响".中国病理生理杂志 26.12(2010):2438-2441.

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