血必净对百草枯诱导人肾近曲小管上皮细胞-2氧化应激和炎症水平的影响

doi:10.3969/j.issn.1008-9691.2019.02.003

科研成果详情

题名	血必净对百草枯诱导人肾近曲小管上皮细胞-2氧化应激和炎症水平的影响
其他题名	Effect of Xuebijing on paraquat-induced cell oxidative stress and inflammation level in human renal proximal convoluted tubular epithelial cell-2
作者	张万里 1; 佘兴蓉 2; 田昕3; 洪广亮2; 曹凯强2; 项公强 1; 卢中秋2
发表日期	2019
发表期刊	中国中西医结合急救杂志影响因子和分区
语种	中文
原始文献类型	期刊论文
关键词	血必净百草枯氧化应激炎症人肾近曲小管上皮细胞-2
其他关键词	Xuebijing ; Paraquat ; Oxidative stress ; Inflammation ; Human kidney proximal convoluted tubular epithelial cell 2
摘要	目的观察血必净注射液对百草枯诱导的人肾近曲小管上皮细胞-2(HK-2)氧化应激和炎症水平的影响。方法常规培养HK-2细胞,按随机数字表法分为空白对照组、百草枯中毒模型组、血必净预处理组、血必净对照组。采用高效液相色谱法(HPLC)检测细胞内百草枯含量;采用化学比色法检测超氧化物歧化酶(SOD)和丙二醛(MDA)水平;采用酶联免疫吸附试验(ELISA)检测白细胞介素-6(IL-6)和肿瘤坏死因子一α (TNF-α )水平。结果随时间延长,百草枯中毒模型组和血必净预处理组HK-2细胞内百草枯含量逐渐升高,作用48 h达峰值,且血必净预处理组HK-2细胞内百草枯含量较百草枯中毒模型组明显降低(mg/L:4.26±0.20比5.77±0.18,P<0.05)。与空白对照组比较,百草枯中毒模型组MDA、TNF-α和IL-6含量明显升高〔MDA(nmol/mg) : 4.47±0.10比2.21 ±0.08,TNF-α (ng/L) : 206.91 ± 13.22比98.14±5.67),IL-6(ng/L):253.33±5.22比116.97± 13.54,均P<0.05〕,SOD活性明显降低(U/mg : 33.30±0.62比41.58±0.17,P<0.05)。与百草枯中毒模型组比较,血必净预处理组HK-2细胞内MDA、TNF-α和IL-6含量明显降低〔MDA(nmol/mg): 2.92±0.17比4.47±0.10,TNF-α (ng/L) : 166.29±15.47比206.91 ± 13.22,IL-6(ng/L) : 209.39±3.18比253.33±5.22,均P<0.05〕,SOD活性明显升高(U/mg : 38.10±0.67比33.30±0.62,P<0.05)。结论血必净可降低HK-2细胞内百草枯含量以及细胞氧化应激和炎症因子水平。
其他摘要	Objective To investigate the effect of Xuebijing on paraquat-induced oxidative stress and inflammation level in human kidney cell line-2 (HK-2) cells. Methods The HK-2 cells were routinely cultured and divided into blank control group, paraquat poisoning model group, Xuebijing injection pretreatment group and Xuebijing control group according to random number table method. The concentration of paraquat in HK-2 cells were measured by high performance liquid chromatography (HPLC); the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) were measured by chemical colorimetry method; the levels of interleukin-6 (IL-6) and tumor necrosis factor- α (TNF- α) were detected by enzyme linked immunosorbent assay (ELISA). Results With prolonged treatment, paraquat concentration gradually increased in HK-2 cells in the paraquat poisoning model group and the Xuebijing injection pretreatment group, reaching the peak value after treatment for 48 hours, moreover, the concentration of paraquat in HK-2 in the Xuebijing injection pretreatment group was significantly lower than that in the paraquat poisoning model group (mg/L: 4.26 ± 0.20 vs. 5.77 ± 0.18,P < 0.05). Compared with the blank control group, the contents of MDA, TNF- α and IL-6 of paraquat poisoning model group were obviously elevated [MDA (nmol/mg): 4.47 ±0.10 vs. 2.21 ±0.08,TNF-α (ng/L): 206.91 ± 13.22 vs. 98.14±5.67,IL-6 (ng/L): 253.33±5.22 vs. 116.97± 13.54,all P < 0.05],and the activity of SOD was significantly reduced (U/mg: 33.30 ±0.62 vs. 41.58 ±0.17,P < 0.05). Compared with paraquat poisoning model group, the contents of MDA, TNF- α and IL-6 in Xuebijing injection pretreatment group were obviously decreased [MDA (nmol/mg): 2.92±0.17 vs. 4.47±0.10,TNF-α (ng/L): 166.29±15.47 vs. 206.91 ± 13.22,IL-6 (ng/L): 209.39 ±3.18 vs. 253.33±5.22, all P < 0.05],and the activity of SOD was significantly increased (U/mg:38.10 ± 0.67 vs. 33.30 ± 0.62, P < 0.05) in the paraquat pretreatment group. Conclusion Xuebijing could reduce the concentration of paraquat in HK-2 cells, and decrease oxidative stress and inflammation factor levels in HK-2 cells.
资助项目	浙江省温州市科技计划项目 ; 浙江省中医药重点学科计划 ; 浙江省“十二五”重点学科建设项目 ; 浙江省医学创新学科建设项目
ISSN	1008-9691
卷号	26 期号:2 页码:143-146
DOI	10.3969/j.issn.1008-9691.2019.02.003
页数	4
收录类别	CSCD ; 万方 ; 维普 ; PKU ; 北大核心 ; ISTIC
学科领域	医药、卫生
URL	查看原文
CSCD记录号	CSCD:6501674
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/98826
专题	附属第一医院其他_附属第五医院（丽水市中心医院）
作者单位	1.温州市龙湾区第一人民医院, 浙江, 温州, 325024 ; 2.温州医科大学附属第一医院急诊医学中心, 浙江, 温州, 325000 ; 3.温州医科大学附属第五医院, 浙江, 丽水, 323000
推荐引用方式 GB/T 7714	张万里,佘兴蓉,田昕,等. 血必净对百草枯诱导人肾近曲小管上皮细胞-2氧化应激和炎症水平的影响[J]. 中国中西医结合急救杂志,2019,26(2):143-146.
APA	张万里., 佘兴蓉., 田昕., 洪广亮., 曹凯强., ... & 卢中秋. (2019). 血必净对百草枯诱导人肾近曲小管上皮细胞-2氧化应激和炎症水平的影响. 中国中西医结合急救杂志, 26(2), 143-146.
MLA	张万里,et al."血必净对百草枯诱导人肾近曲小管上皮细胞-2氧化应激和炎症水平的影响".中国中西医结合急救杂志 26.2(2019):143-146.