DETERMINATION OF NORDIHYDROCAPSAICIN IN RABBIT PLASMA BY LIQUID CHROMATOGRAPHY-ELECTROSPRAY MASS SPECTROMETRY

doi:10.1080/10826076.2013.850721

科研成果详情

题名	DETERMINATION OF NORDIHYDROCAPSAICIN IN RABBIT PLASMA BY LIQUID CHROMATOGRAPHY-ELECTROSPRAY MASS SPECTROMETRY
作者	Lin, Guanyang1; Ma, Jianshe2; Lin, Chongliang1; Hu, Lufeng1; Wang, Xianqin3
发表日期	2014-12-31
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	determination intravenous LC-MS nordihydrocapsaicin pharmacokinetics rabbit plasma
其他关键词	QUANTITATIVE-ANALYSIS ; CAPSAICINOIDS ; PEPPERS ; DIHYDROCAPSAICIN ; QUANTIFICATION ; NONIVAMIDE ; CAPSICUM ; MS
摘要	A simple and selective liquid chromatography mass spectrometry (LC-MS) method for determination of nordihydrocapsaicin in rabbit plasma was developed and validated over the concentration range of 10-2000 ng/mL. After addition of midazolam as internal standard (IS), protein precipitation with acetonitrile was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1mm x 150 mm, 5.0 mu m) column with acetonitrile-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used for quantification using target fragment ions m/z 294 for nordihydrocapsaicin and m/z 326 for the IS. Lower limit of quantification (LLOQ) for nordihydrocapsaicin was 10 ng/mL. Mean recovery of nordihydrocapsaicin from plasma was in the range 92.0-96.6%. Relative standard deviation (RSD) of intra-day and inter-day precision were both less than 9%. The LC-MS method successfully applied to a pharmacokinetic study of nordihydrocapsaicin after intravenous administration of single dosage 5mg/kg to rabbits.
资助项目	Youth Talent Program Foundation of The First Affiliated Hospital of Wenzhou Medical University [qnyc010]
出版者	TAYLOR & FRANCIS INC
出版地	PHILADELPHIA
ISSN	1082-6076
EISSN	1520-572X
卷号	37 期号:17 页码:2506-2514
DOI	10.1080/10826076.2013.850721
页数	9
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000337239300009
收录类别	SCIE ; EI ; SCOPUS ; 万方
EI入藏号	20142217766676
EI主题词	Liquid chromatography
URL	查看原文
SCOPUSEID	2-s2.0-84901257248
通讯作者地址	[Wang, Xianqin]Analytical and Testing Center, Wenzhou Medical University,China
Scopus学科分类	Analytical Chemistry;Biochemistry;Pharmaceutical Science;Clinical Biochemistry
万方分类号	O661(化学)
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/965
专题	基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）温州医科大学附属第一医院
通讯作者	Wang, Xianqin
作者单位	1.Function Experiment Teaching Center, Wenzhou Medical University,China; 2.First Affiliated Hospital, Wenzhou Medical University,China; 3.Analytical and Testing Center, Wenzhou Medical University,China
第一作者单位	基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）
通讯作者单位	温州医科大学
第一作者的第一单位	基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）
推荐引用方式 GB/T 7714	Lin, Guanyang,Ma, Jianshe,Lin, Chongliang,et al. DETERMINATION OF NORDIHYDROCAPSAICIN IN RABBIT PLASMA BY LIQUID CHROMATOGRAPHY-ELECTROSPRAY MASS SPECTROMETRY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(17):2506-2514.
APA	Lin, Guanyang, Ma, Jianshe, Lin, Chongliang, Hu, Lufeng, & Wang, Xianqin. (2014). DETERMINATION OF NORDIHYDROCAPSAICIN IN RABBIT PLASMA BY LIQUID CHROMATOGRAPHY-ELECTROSPRAY MASS SPECTROMETRY. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 37(17), 2506-2514.
MLA	Lin, Guanyang,et al."DETERMINATION OF NORDIHYDROCAPSAICIN IN RABBIT PLASMA BY LIQUID CHROMATOGRAPHY-ELECTROSPRAY MASS SPECTROMETRY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 37.17(2014):2506-2514.