Simultaneous determination of amiodarone, dronedarone, and their principal metabolites in SD rat plasma by UPLC-MS/MS and its application in pharmacokinetics

doi:10.1016/j.arabjc.2021.103300

科研成果详情

题名	Simultaneous determination of amiodarone, dronedarone, and their principal metabolites in SD rat plasma by UPLC-MS/MS and its application in pharmacokinetics
作者	Zhang, Ying 1,2; Zhu, Mengxiao 3; Xie, Saili4; Ye, Xuemei4; Xu, Xuegu5
发表日期	2021-08
发表期刊	ARABIAN JOURNAL OF CHEMISTRY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Amiodarone Dronedarone Pharmacokinetics UPLC-MS MS Rat plasma
其他关键词	DESETHYLAMIODARONE
摘要	The purpose of the experiment was mainly to establish and verify a precise and straightforward ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) approach for simultaneously analyzing the concentration levels of amiodarone, dronedarone, and their metabolites (desethylamiodarone and desbutyldronedarone) in the plasma of SpragueDawley (SD) rats and to investigate the pharmacokinetics of all analytes in SD rats. After rapid protein precipitation by using acetonitrile, we accomplished the chromatographic separation of amiodarone, desethylamiodarone, dronedarone, desbutyldronedarone and ivabradine (internal standard, IS) by using Acquity BEH C18 column and detected through a mass spectrometer with Xevo TQ-S triple quadrupole tandem, choosing the positive ion mode. The approach showed wonderful linearity, and the range of calibration curve for amiodarone was 1-200 ng/mL, desethylamiodarone was 0.1-20 ng/mL, dronedarone was 0.5-100 ng/mL, and desbutyldronedarone was 0.25- 50 ng/mL, respectively. For lower limit of quantification (LLOQ), the current method of UPLCMS/MS can achieve values of 1.0 ng/mL for amiodarone, 0.1 ng/mL for desethylamiodarone, 0.5 ng/mL for dronedarone, and 0.25 ng/mL for desbutyldronedarone, respectively. The accuracy of intra-day and inter-day of all analytes was between-14.8% to 10.9%, while the precision was < 13.3%. For each substance, the recovery rate was > 82.1%, besides, obvious matrix effect was not found. In all conditions, the stability of all analytes was comfirmed to the plasma sample quantification. In addition, the method of UPLC-MS/MS we developed could also be applied to measure the pharmacokinetic characteristics including amiodarone, desethylamiodarone, dronedarone, and desbutyldronedarone in the plasma of SD rats. (c) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
出版者	ELSEVIER
出版地	AMSTERDAM
ISSN	1878-5352
EISSN	1878-5379
卷号	14 期号:8
DOI	10.1016/j.arabjc.2021.103300
页数	8
WOS类目	Chemistry, Multidisciplinary
WOS研究方向	Chemistry
WOS记录号	WOS:000677946200009
收录类别	SCIE ; EI ; SCOPUS
EI入藏号	20212810625367
EI主题词	Rats
URL	查看原文
SCOPUSEID	2-s2.0-85109428172
通讯作者地址	[Ye, Xuemei]The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Chemistry (all);Chemical Engineering (all)
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/9045
专题	附属第一医院眼视光学院（生物医学工程学院）、附属眼视光医院
通讯作者	Ye, Xuemei
作者单位	1.Department of Pharmacy,HwaMei Hospital,University of Chinese Academy of Sciences (Ningbo No.2 Hospital),Ningbo,315010,China; 2.Ningbo Institute of Life and Health Industry,University of Chinese Academy of Sciences,Ningbo,315010,China; 3.Daping Hospital,Army Medical University,Chongqing,401120,China; 4.The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 5.The Eye Hospital of Wenzhou Medical University,Wenzhou,325000,China
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
推荐引用方式 GB/T 7714	Zhang, Ying,Zhu, Mengxiao,Xie, Saili,et al. Simultaneous determination of amiodarone, dronedarone, and their principal metabolites in SD rat plasma by UPLC-MS/MS and its application in pharmacokinetics[J]. ARABIAN JOURNAL OF CHEMISTRY,2021,14(8).
APA	Zhang, Ying, Zhu, Mengxiao, Xie, Saili, Ye, Xuemei, & Xu, Xuegu. (2021). Simultaneous determination of amiodarone, dronedarone, and their principal metabolites in SD rat plasma by UPLC-MS/MS and its application in pharmacokinetics. ARABIAN JOURNAL OF CHEMISTRY, 14(8).
MLA	Zhang, Ying,et al."Simultaneous determination of amiodarone, dronedarone, and their principal metabolites in SD rat plasma by UPLC-MS/MS and its application in pharmacokinetics".ARABIAN JOURNAL OF CHEMISTRY 14.8(2021).