Deletion of the Igf1 Gene: Suppressive Effects on Adult Leydig Cell Development

doi:10.2164/jandrol.109.008680

科研成果详情

题名	Deletion of the Igf1 Gene: Suppressive Effects on Adult Leydig Cell Development
作者	Hu, Guo-Xin1,2; Lin, Han 3; Chen, Guo-Rong4; Chen, Bing-Bing2; Lian, Qing-Quan3; Hardy, Dianne O.1; Zirkin, Barry R.5; Ge, Ren-Shan1,2
发表日期	2010-07
发表期刊	JOURNAL OF ANDROLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Developmental lineage proliferation testosterone
其他关键词	GROWTH-FACTOR-I ; HYPOPHYSECTOMIZED RATS ; PRIMARY CULTURE ; RECEPTOR GENE ; INSULIN ; TESTIS ; MOUSE ; STEROIDOGENESIS ; IDENTIFICATION ; EXPRESSION
摘要	Deletion of the insulin-like growth factor 1 (Igf1) gene was shown in previous studies to result in reduced numbers of Leydig cells in the testes of 35-day-old mice, and in reduced circulating testosterone levels. In the current study, we asked whether deletion of the Igf1 gene affects the number, proliferation, and/or steroidogenic function of some or all of the precursor cell types in the developmental sequence that leads to the establishment of adult Leydig cells (ALCs). Decreased numbers of cells in the Leydig cell lineage (ie, 3 beta-hydroxysteroid dehydrogenase-positive cells) were seen in testes of postnatal day (PND) 14-90 Igf1(-/-) mice compared with age-matched Igf1(+/+) controls. The development of ALCs proceeds from stem Leydig cells (SLCs) through progenitor Leydig cells (PLCs) and immature Leydig cells (ILCs). The bromodeoxyuridine labeling index of putative SLCs was similar in the Igf1(-/-) and Igf1(+/+) mice. In contrast, the labeling index of PLCs was reduced in the Igf1(-/-) mice on each day of PND 14 through PND 35, and that of more mature Leydig cells (referred to herein as LCs, a combination of ILCs plus ALCs) was reduced from PND 21 through PND 56. In Igf1(-/-) mice that received recombinant IGF-I, the labeling indices of PLCs and LCs were similar to those of age-matched Igf1(+/+) mice, indicating that the reductions in the labeling indices seen in the PLCs and LCs of the Igf1(-/-) mice were a consequence of reduced IGF-I. On each day of PND 21 through PND 90, testicular testosterone concentrations were significantly reduced in the Igf1(-/-) mice, as were the expressions of testis-specific mRNAs involved in steroidogenesis, including Star, Cyp11a1, and Cyp17a1. The increased expression of the gene for 5 alpha-reductase (Srd5a1) in adult Igf1(-/-) testes suggests that the depletion of Igf1 might suppress or delay Leydig cell maturation. These observations, taken together, indicate that the reduced numbers of Leydig cells in the adult testes of Igf1(-/-) mice result at least in part from altered proliferation and differentiation of ALC precursor cells, but not of the stem cells that give rise to these cells.
资助项目	National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [RO1 HD050570, AG030598]; EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENTUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) [R01HD050570] Funding Source: NIH RePORTER; NATIONAL INSTITUTE ON AGINGUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute on Aging (NIA) [R01AG021092, R01AG030598, R37AG021092] Funding Source: NIH RePORTER
出版者	AMER SOC ANDROLOGY, INC
出版地	LAWRENCE
ISSN	0196-3635
EISSN	1939-4640
卷号	31 期号:4 页码:379-387
DOI	10.2164/jandrol.109.008680
页数	9
WOS类目	Andrology
WOS研究方向	Endocrinology & Metabolism
WOS记录号	WOS:000281875700008
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	20203337
SCOPUSEID	2-s2.0-77957852136
通讯作者地址	[Ge, Ren-Shan]Center for Biomedical Research,Population Council,United States
Scopus学科分类	Endocrinology, Diabetes and Metabolism;Reproductive Medicine;Endocrinology;Urology
引用统计	被引频次：53[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8874
专题	药学院（分析测试中心）附属第二医院附属第一医院药学院（分析测试中心）_临床药学与药理系第二临床医学院、附属第二医院、育英儿童医院
通讯作者	Ge, Ren-Shan
作者单位	1.Center for Biomedical Research,Population Council,United States; 2.Department of Pharmacology,School of Pharmacy,Wenzhou Medical College,China; 3.2nd Affiliated Hospital,Wenzhou Medical College,China; 4.1st Affiliated Hospital,Wenzhou Medical College,China; 5.Department of Biochemistry and Molecular Biology,Johns Hopkins University,Bloomberg School of Public Health,United States
第一作者单位	临床药学与药理系
推荐引用方式 GB/T 7714	Hu, Guo-Xin,Lin, Han,Chen, Guo-Rong,et al. Deletion of the Igf1 Gene: Suppressive Effects on Adult Leydig Cell Development[J]. JOURNAL OF ANDROLOGY,2010,31(4):379-387.
APA	Hu, Guo-Xin., Lin, Han., Chen, Guo-Rong., Chen, Bing-Bing., Lian, Qing-Quan., ... & Ge, Ren-Shan. (2010). Deletion of the Igf1 Gene: Suppressive Effects on Adult Leydig Cell Development. JOURNAL OF ANDROLOGY, 31(4), 379-387.
MLA	Hu, Guo-Xin,et al."Deletion of the Igf1 Gene: Suppressive Effects on Adult Leydig Cell Development".JOURNAL OF ANDROLOGY 31.4(2010):379-387.