Bioinformatic analysis of the Acinetobacter baumannii phage AB1 genome

doi:10.1016/j.gene.2012.07.029

科研成果详情

题名	Bioinformatic analysis of the Acinetobacter baumannii phage AB1 genome
作者	Li, Peizhen1; Chen, Biaobang 1; Song, Zhijian 1; Song, Yulong 1; Yang, Yanmei 1; Ma, Ping1; Wang, Huifeng 1; Ying, Jun1; Ren, Ping 1; Yang, Lei 1; Gao, Guohui1; Jin, Shouguang 3; Bao, Qiyu1; Yang, Hongjiang 2
发表日期	2012-10-10
发表期刊	GENE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Acinetobacter baumannii Bacteriophage Genome sequencing Genome annotation Phylogenetic tree
其他关键词	C-TERMINAL DOMAIN ; BACTERIOPHAGE ; EVOLUTION ; RESISTANT ; DNA ; DIVERSITY ; ALIGNMENT ; TOOL
摘要	As one of the pathogens of hospital-acquired infections, Acinetobacter baumannii poses great challenges to the public health. A. baumannii phage could be an effective way to fight multi-resistant A. baumannii. Here, we completed the whole genome sequencing of the complete genome of A. baumannii phage AB1, which consists of 45,159 bp and is a double-stranded DNA molecule with an average GC content of 37.7%. The genome encodes one tRNA gene and 85 open reading frames (ORFs) and the average size of the ORF is 531 bp in length. Among 85 ORFs, only 14 have been identified to share significant sequence similarities to the genes with known functions, while 28 are similar in sequence to the genes with function-unknown genes in the database and 43 ORFs are uniquely present in the phage AB1 genome. Fourteen function-assigned genes with putative functions include five phage structure proteins, an RNA polymerase, a big sub-unit and a small sub-unit of a terminase, a methylase and a recombinase and the proteins involved in DNA replication and so on. Multiple sequence alignment was conducted among those homologous proteins and the phylogenetic trees were reconstructed to analyze the evolutionary courses of these essential genes. From comparative genomics analysis, it turned out clearly that the frame of the phage genome mainly consisted of genes from Xanthomonas phages, Burkholderia ambifaria phages and Enterobacteria phages and while it comprises genes of its host A. baumannii only sporadically. The mosaic feature of the phage genome suggested that the horizontal gene transfer occurred among the phage genomes and between the phages and the host bacterium genomes. Analyzing the genome sequences of the phages should lay sound foundation to investigate how phages adapt to the environment and infect their hosts, and even help to facilitate the development of biological agents to deal with pathogenic bacteria. (c) 2012 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31071115]; Science and Technology Foundation of Zhejiang Province [2009C33040]; Science and Technology Foundation of Wenzhou City [S20090002]; Foundation of Science and Technology Innovation Team of Zhejiang Province, China [2010R50048]
出版者	ELSEVIER
出版地	AMSTERDAM
ISSN	0378-1119
EISSN	1879-0038
卷号	507 期号:2 页码:125-134
DOI	10.1016/j.gene.2012.07.029
页数	10
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000308515000005
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	22868206
SCOPUSEID	2-s2.0-84865196564
通讯作者地址	[Bao, Qiyu]Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics,Wenzhou Medical College,China
Scopus学科分类	Genetics
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8838
专题	温州医科大学
通讯作者	Bao, Qiyu
作者单位	1.Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics,Wenzhou Medical College,China; 2.Key Laboratory of Industrial Microbiology,Ministry of Education,Tianjin University of Science and Technology,P.O. Box 08, TEDA,China; 3.Department of Molecular Genetics and Microbiology,University of Florida,United States
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Li, Peizhen,Chen, Biaobang,Song, Zhijian,et al. Bioinformatic analysis of the Acinetobacter baumannii phage AB1 genome[J]. GENE,2012,507(2):125-134.
APA	Li, Peizhen., Chen, Biaobang., Song, Zhijian., Song, Yulong., Yang, Yanmei., ... & Yang, Hongjiang. (2012). Bioinformatic analysis of the Acinetobacter baumannii phage AB1 genome. GENE, 507(2), 125-134.
MLA	Li, Peizhen,et al."Bioinformatic analysis of the Acinetobacter baumannii phage AB1 genome".GENE 507.2(2012):125-134.