Circular RNA sequencing indicates circ-IQGAP2 and circ-ZC3H6 as noninvasive biomarkers of primary Sjogren's syndrome

doi:10.1093/rheumatology/keaa163

科研成果详情

题名	Circular RNA sequencing indicates circ-IQGAP2 and circ-ZC3H6 as noninvasive biomarkers of primary Sjogren's syndrome
作者	Li, Fengxia 1; Liu, Zhenwei1; Zhang, Bing 1; Jiang, Shan 1; Wang, Qiongdan 1,2; Du, Lifeng 1; Xue, Huangqi 1; Zhang, Yu 1; Jin, Mengmeng3; Zhu, Xiaochun3; Brown, Matthew A 4,5; Wu, Jinyu 1; Wang, Xiaobing3
发表日期	2020-09
发表期刊	RHEUMATOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	primary Sjogren's syndrome RNA sequencing circular RNAs circ-IQGAP2 circ-ZC3H6
其他关键词	SALIVARY-GLAND INFLAMMATION ; CLASSIFICATION CRITERIA ; EXPRESSION PROFILES ; AMERICAN-COLLEGE ; DATA-DRIVEN ; MIR-142-3P ; CONSENSUS ; DIFFERENTIATION ; MACROPHAGE ; MICRORNAS
摘要	Objectives. This study aims to characterize the expression profiles of circRNAs in primary Sjogren's Syndrome (pSS) and examine the potential of noninvasive circular RNAs (circRNAs) as biomarkers of pSS. Methods. We performed RNA sequencing of minor salivary gland (MSG) biopsies from four pSS and four non-pSS individuals (subjects undergoing MSG biopsies but not meeting 2012 or 2016 ACR classification criteria for SS). Differentially expressed circRNAs were identified by DESeq2, and confirmed by quantitative real-time PCR in the MSGs as well as in plasma exosomes in 37 pSS and 14 non-pSS subjects. Discriminatory capacity testing using receiver operating characteristic analysis was used to evaluate the performance of circRNAs as diagnostic biomarkers for pSS. Results. Circ-IQGAP2 and circ-ZC3H6 had significantly upregulated expression in the MSGs of pSS patients, and this elevated expression was confirmed by quantitative real-time PCR of plasma exosome RNA. The expression of these circRNAs also showed significant correlation with both clinical features, serum IgG level and MSG focus scores. Receiver operating characteristic analysis showed that the indices comprised of both the two circRNAs and clinical features were better able to distinguish pSS from non-pSS subjects with high mean areas under the curve of 0.93 in the MSGs and 0.92 in the plasma exosomes. Conclusion. This study indicated the potential roles of circ-IQGAP2 and circ-ZC3H6 as noninvasive biomarkers for the diagnosis of pSS.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81700062]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LQ16H010003]; Science and Technology Project of Zhejiang Provincial Health Commission [2019RC050]; Science and Technology Project of Wenzhou [Y20160028]; Plan of Scientific and technological Innovation activities for College students in Zhejiang Province [2019R413082]; General scientific research projects of Zhejiang Education Department [Y201942208]
出版者	OXFORD UNIV PRESS
出版地	OXFORD
ISSN	1462-0324
EISSN	1462-0332
卷号	59 期号:9 页码:2603-2615
DOI	10.1093/rheumatology/keaa163
页数	13
WOS类目	Rheumatology
WOS研究方向	Rheumatology
WOS记录号	WOS:000574394600059
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	32250392
Scopus记录号	2-s2.0-85090076618
通讯作者地址	[Wang, Xiaobing]Institute of Genomic Medicine,Wenzhou Medical University,China
scopus学科分类	Rheumatology;Pharmacology (medical)
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8778
专题	基因组医学研究院附属第一医院_风湿免疫科第一临床医学院（信息与工程学院）、附属第一医院_临床检验诊断学第一临床医学院（信息与工程学院）、附属第一医院_精准医学中心实验室
通讯作者	Wang, Xiaobing
作者单位	1.Institute of Genomic Medicine,Wenzhou Medical University,China; 2.Department of Clinical Laboratory,First Affiliated Hospital of Wenzhou Medical University,China; 3.Department of Rheumatology,First Affiliated Hospital,Wenzhou Medical University,Wenzhou,China; 4.Guy's and St Thomas Nhs Foundation Trust and King's,College London Nihr Biomedical Research Centre,London,United Kingdom; 5.Centre for Precision Medicine,First Affiliated Hospital,Wenzhou Medical University,Wenzhou,China
第一作者单位	基因组医学研究院
通讯作者单位	基因组医学研究院
第一作者的第一单位	基因组医学研究院
推荐引用方式 GB/T 7714	Li, Fengxia,Liu, Zhenwei,Zhang, Bing,et al. Circular RNA sequencing indicates circ-IQGAP2 and circ-ZC3H6 as noninvasive biomarkers of primary Sjogren's syndrome[J]. RHEUMATOLOGY,2020,59(9):2603-2615.
APA	Li, Fengxia., Liu, Zhenwei., Zhang, Bing., Jiang, Shan., Wang, Qiongdan., ... & Wang, Xiaobing. (2020). Circular RNA sequencing indicates circ-IQGAP2 and circ-ZC3H6 as noninvasive biomarkers of primary Sjogren's syndrome. RHEUMATOLOGY, 59(9), 2603-2615.
MLA	Li, Fengxia,et al."Circular RNA sequencing indicates circ-IQGAP2 and circ-ZC3H6 as noninvasive biomarkers of primary Sjogren's syndrome".RHEUMATOLOGY 59.9(2020):2603-2615.