Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*

doi:10.3967/bes2021.074

科研成果详情

题名	Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*
作者	JIA, Rong 1,2; CHEN, Ya Xiong 1; DU, Ya Rong 1; HU, Bu Rong 1,2,3
发表日期	2021-07
发表期刊	BIOMEDICAL AND ENVIRONMENTAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	MSD assay Low or high LET irradiation Mouse plasma cytokines Dose estimation model
其他关键词	CHROMOSOME-ABERRATION ANALYSIS ; MICRONUCLEUS ASSAY ; RADIATION ; BIODOSIMETRY ; EXPOSURE ; ELISA ; MODULATION ; WORKERS ; HUMANS ; CANCER
摘要	Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer (LET) irradiation and to develop an accurate model of radiation exposure based on the cytokine levels after irradiation. Methods Plasma samples harvested from mice at different time points after carbon-ion or X-ray irradiation were analyzed using meso-scale discovery (MSD), a high-throughput and sensitive electrochemiluminescence measurement technique. Dose estimation equations were set up using multiple linear regression analysis. Results The relative levels of IL-6 at 1 h, IL-5 and IL-6 at 24 h, and IL-5, IL-6 and IL-15 at 7 d after irradiation with two intensities increased dose-dependently. The minimum measured levels of IL-5, IL-6 and IL-15 were up to 4.0076 pg/mL, 16.4538 pg/mL and 0.4150 pg/mL, respectively. In addition, dose estimation models were established and verified. Conclusions The MSD assay can provide more accurate data regarding the changes in the levels of the cytokines IL-5, IL-6 and IL-15. These cytokines could meet the essential criteria for radiosensitive biomarkers and can be used as radiation indicators. Our prediction models can conveniently and accurately estimate the exposure dose in irradiated organism.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [11635013, 11705248, U1832101]; National Key Research and Development Program of China [2017YFC0108605]; Science and Technology Research Project of Gansu Province [145RTSA012, 17JR5RA307]; Heavy Ion Research Facility in Lanzhou [Y9HIRFL200]
出版者	CHINESE CENTER DISEASE CONTROL & PREVENTION
出版地	BEIJING
ISSN	0895-3988
EISSN	2214-0190
卷号	34 期号:7 页码:540-551
DOI	10.3967/bes2021.074
页数	12
WOS类目	Environmental Sciences ; Public, Environmental & Occupational Health
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
WOS记录号	WOS:000691530100004
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	34353417
Scopus记录号	2-s2.0-85113345443
通讯作者地址	[HU, Bu Rong]Key Laboratory of Space Radiobiology of Gansu Province & CAS Key Laboratory of Heavy Ion Radiation Biology and Medicine,Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou,730000,China
scopus学科分类	Public Health, Environmental and Occupational Health;Health, Toxicology and Mutagenesis
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8737
专题	公共卫生与管理学院_放射卫生与放射医学系
通讯作者	HU, Bu Rong
作者单位	1.Key Laboratory of Space Radiobiology of Gansu Province & CAS Key Laboratory of Heavy Ion Radiation Biology and Medicine,Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou,730000,China; 2.School of Nuclear Science and Technology,University of Chinese Academy of Sciences,Beijing,100039,China; 3.Department of Radiation Medicine,School of Public Health and Management,Wenzhou Medical University,Wenzhou,325035,China
推荐引用方式 GB/T 7714	JIA, Rong,CHEN, Ya Xiong,DU, Ya Rong,et al. Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2021,34(7):540-551.
APA	JIA, Rong, CHEN, Ya Xiong, DU, Ya Rong, & HU, Bu Rong. (2021). Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*. BIOMEDICAL AND ENVIRONMENTAL SCIENCES, 34(7), 540-551.
MLA	JIA, Rong,et al."Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 34.7(2021):540-551.

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