Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia-Reperfusion Injury

doi:10.1007/s12012-012-9179-6

科研成果详情

题名	Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia-Reperfusion Injury
作者	Gong, Dingxu 1,2,3,4,5; Zhang, Yujian6; Zhang, Hao 1,2,3,4,5; Gu, Haiyong 1,2,3,4,5; Jiang, Qin 1,2,3,4,5; Hu, Shengshou 1,2,3,4,5
发表日期	2012-12
发表期刊	CARDIOVASCULAR TOXICOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Cardioprotection Aldehyde dehydrogenase-2 Ischemia and reperfusion Cardioplegia solution
其他关键词	DAMAGE ; INACTIVATION ; INHIBITION ; PROTEINS ; THERAPY ; SURGERY
摘要	Ischemia/reperfusion damage is common during open-heart surgery. Activation of aldehyde dehydrogenase-2 can significantly reduce ischemia/reperfusion damage. We hypothesized that adding aldehyde dehydrogenase-2 agonist to regular cardioplegia solution would further ameliorate ischemia/reperfusion damage. Alda-1 was used as an aldehyde dehydrogenase-2 agonist. Cardioprotection by histidine-tryptophan-ketoglutarate solution with and without Alda-1 was compared using an ex vivo perfused rat heart model of ischemia/reperfusion. Three groups of ex vivo rat hearts endured different treatments with variant ischemia or an ischemia/reperfusion time course: sham, no ischemia/reperfusion; histidine-tryptophan-ketoglutarate; and histidine-tryptophan-ketoglutarate plus Alda-1. Aldehyde dehydrogenase-2 expressions and activities, oxidative parameters (including 4-hydroxy-2-nonenal-His adducts, malondialdehyde levels, and glutathione/oxidized glutathione ratios), myocardial protein carbonyl levels, coronary effluents creatine kinase isoenzyme MB levels, and heart function parameters were measured and compared. Alda-1 significantly elevated myocardium aldehyde dehydrogenase-2 activity (P < .01). Increased aldehyde dehydrogenase-2 activity in turn attenuated ischemia/reperfusion-induced elevation in cardiac aldehydes, creatine kinase isoenzyme MB leakage, and protein carbonyl formation (P < .01). The Alda-1 group also obtained higher glutathione/oxidized glutathione ratios (P < .01). Aldehyde dehydrogenase-2 activation alleviated ischemia/reperfusion-induced cardiomyocyte contractile function impairment as evidenced by improved maximal velocity of pressure development and decline, left ventricular developed pressure, and heart rate (P < .01). Alda-1 supplementation can significantly improve the cardioprotection effect of cardioplegia solution, possibly through activation of aldehyde dehydrogenase-2, to remove toxic aldehydes. This may aid in the identification of novel cardioplegia solutions.
资助项目	Ministry of Science and Technology, ChinaMinistry of Science and Technology, China [2010CB529500]; Program for Innovation Research Team in Peking Union Medical College
出版者	HUMANA PRESS INC
出版地	TOTOWA
ISSN	1530-7905
EISSN	1559-0259
卷号	12 期号:4 页码:350-358
DOI	10.1007/s12012-012-9179-6
页数	9
WOS类目	Cardiac & Cardiovascular Systems ; Toxicology
WOS研究方向	Cardiovascular System & Cardiology ; Toxicology
WOS记录号	WOS:000311361400009
收录类别	SCIE ; SCOPUS
URL	查看原文
Pubmed记录号	22814936
Scopus记录号	2-s2.0-84870564342
通讯作者地址	[Zhang, Hao]Chinese Acad Med Sci, Natl Ctr Cardiovasc Dis, State Key Lab Cardiovasc Dis, Fuwai Hosp, Beijing 100037, Peoples R China.
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8639
专题	附属第一医院_麻醉科
通讯作者	Zhang, Hao
作者单位	1.Chinese Acad Med Sci, Natl Ctr Cardiovasc Dis, State Key Lab Cardiovasc Dis, Fuwai Hosp, Beijing 100037, Peoples R China; 2.Peking Union Med Coll, Beijing 100037, Peoples R China; 3.Chinese Acad Med Sci, Fuwai Hosp, Dept Surg, Beijing 100037, Peoples R China; 4.Chinese Acad Med Sci, Fuwai Hosp, Ctr Pediat Cardiac Surg, Beijing 100037, Peoples R China; 5.Chinese Acad Med Sci, Cardiovasc Inst, Beijing 100037, Peoples R China; 6.Wenzhou Med Coll, Dept Anesthesiol, Affiliated Hosp 1, Wenzhou, Peoples R China
推荐引用方式 GB/T 7714	Gong, Dingxu,Zhang, Yujian,Zhang, Hao,et al. Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia-Reperfusion Injury[J]. CARDIOVASCULAR TOXICOLOGY,2012,12(4):350-358.
APA	Gong, Dingxu, Zhang, Yujian, Zhang, Hao, Gu, Haiyong, Jiang, Qin, & Hu, Shengshou. (2012). Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia-Reperfusion Injury. CARDIOVASCULAR TOXICOLOGY, 12(4), 350-358.
MLA	Gong, Dingxu,et al."Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia-Reperfusion Injury".CARDIOVASCULAR TOXICOLOGY 12.4(2012):350-358.