Tissue extracts from infarcted myocardium of rats in promoting the differentiation of bone marrow stromal cells into cardiomyocyte-like cells

doi:10.1016/S0895-3988(08)60015-X

科研成果详情

题名	Tissue extracts from infarcted myocardium of rats in promoting the differentiation of bone marrow stromal cells into cardiomyocyte-like cells
作者	Liu, Xiao-Ning 1,2,3; Yin, Qi1,2,3,4; Zhang, Hao 1,2,3; Zhang, Hong 1,2,3; Zhu, Shen-Jun 1,2,3; Wei, Ying-Jie 1,2,3; Hu, Sheng-Shou 1,2,3
发表日期	2008-04
发表期刊	BIOMEDICAL AND ENVIRONMENTAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	bone marrow stromal cells cell differentiation cardiac tissue extracts myocardial infarction
其他关键词	MESENCHYMAL STEM-CELLS ; TRANSPLANTATION ; EXPRESSION ; PHENOTYPE ; APOPTOSIS ; CONTACT ; NKX2-5 ; REPAIR
摘要	Objective To investigate whether cardiac tissue extracts from rats could mimic the cardiac microenvironment and act as a natural inducer in promoting the differentiation of bone marrow stromal cells (BMSCs) into cardiomyocytes. Methods Three kinds of tissue extract or cell lysate [infarcted myocardial tissue extract (IMTE), normal myocardial tissue extract (NMTE) and cultured neonatal myocardial lysate (NML)] were employed to induce BMSCs into cardiomyocyte-like cells. The cells were harvested at each time point for reverse transcription-polymerase chain reaction (RT-PCR) detection, immunocytochemical analysis, and transmission electron microscopy. Results After a 7-day induction, BMSCs were enlarged and polygonal in morphology. Myofilaments, striated sarcomeres, Z-lines, and more mitochondia were observed under transmission electron microscope. Elevated expression levels of cardiac-specific genes and proteins were also confirmed by RT-PCR and immunocytochemistry. Moreover, IMTE showed a greater capacity of differentiating BMSCs into cardiomyocyte-like cells. Conclusions Cardiac tissue extracts, especially IMTE, can effectively differentiate BMSCs into cardiomyocyte-like cells.
资助项目	National Natural Science Foundation of China[30570722];
出版者	CHINESE CENTER DISEASE CONTROL & PREVENTION
出版地	BEIJING
ISSN	0895-3988
卷号	21 期号:2 页码:110-117
DOI	10.1016/S0895-3988(08)60015-X
页数	8
WOS类目	Environmental Sciences ; Public, Environmental & Occupational Health
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
WOS记录号	WOS:000254850400004
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	18548849
SCOPUSEID	2-s2.0-43949136429
通讯作者地址	[Wei, Ying-Jie]Chinese Acad Med Sci, Cardiovasc Inst Fuwai Hosp, Key Lab Cardiovasc Regenerat Med, Minist Hlth, Beijing 100037, Peoples R China.
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8603
专题	附属第一医院_心胸外科
通讯作者	Wei, Ying-Jie
作者单位	1.Chinese Acad Med Sci, Cardiovasc Inst Fuwai Hosp, Key Lab Cardiovasc Regenerat Med, Minist Hlth, Beijing 100037, Peoples R China; 2.Chinese Acad Med Sci, Dept Cardiovasc Surg, Beijing 100037, Peoples R China; 3.Peking Union Med Coll, Beijing 100037, Peoples R China; 4.Wenzhou Med Coll, Affiliated Hosp 1, Dept Thorac & Cardiovasc Surg, Wenzhou 325000, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiao-Ning,Yin, Qi,Zhang, Hao,et al. Tissue extracts from infarcted myocardium of rats in promoting the differentiation of bone marrow stromal cells into cardiomyocyte-like cells[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2008,21(2):110-117.
APA	Liu, Xiao-Ning., Yin, Qi., Zhang, Hao., Zhang, Hong., Zhu, Shen-Jun., ... & Hu, Sheng-Shou. (2008). Tissue extracts from infarcted myocardium of rats in promoting the differentiation of bone marrow stromal cells into cardiomyocyte-like cells. BIOMEDICAL AND ENVIRONMENTAL SCIENCES, 21(2), 110-117.
MLA	Liu, Xiao-Ning,et al."Tissue extracts from infarcted myocardium of rats in promoting the differentiation of bone marrow stromal cells into cardiomyocyte-like cells".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 21.2(2008):110-117.