Prevent diabetic cardiomyopathy in diabetic rats by combined therapy of aFGF-loaded nanoparticles and ultrasound-targeted microbubble destruction technique

doi:10.1016/j.jconrel.2015.12.030

科研成果详情

题名	Prevent diabetic cardiomyopathy in diabetic rats by combined therapy of aFGF-loaded nanoparticles and ultrasound-targeted microbubble destruction technique
作者	Zhao, Ying-Zheng1,2; Zhang, Ming 1; Wong, Ho Lun 3; Tian, Xin-Qiao 4; Zheng, Lei5; Yu, Xi-Chong1; Tian, Fu-Rong 1; Mao, Kai-Li1; Fan, Zi-Liang 1; Chen, Pian-Pian 1; Li, Xiao-Kun1; Lu, Cui-Tao1,2
发表日期	2016-02-10
发表期刊	JOURNAL OF CONTROLLED RELEASE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Preventive strategy Diabetic cardiomyopathy aFGF Nanoparticles Ultrasound-targeted microbubble destruction technique
其他关键词	INTRACELLULAR DELIVERY ; DRUG-DELIVERY ; GENE-THERAPY ; SONOPORATION ; CAVITATION ; PROTEIN ; ISCHEMIA ; PROMOTES ; MELLITUS ; INJURY
摘要	Acidic fibroblast growth factor (aFGF) has shown the great potential to prevent the structural and functional injuries caused by diabetic cardiomyopathy (DCM). The present study sought to investigate the preclinical performance and mechanism of the combination therapy of aFGF-nanoparticles (aFGF-NP) and ultrasound-targeted microbubble destruction (UTMD) technique for DCM prevention. From Mason staining and TUNEL staining, aFGF-NP + UTMD group showed significant differences from the diabetes group and other groups treated with aFGF or aFGF-NP. The cardiac collagen volume fraction (CVF) and cardiac myocyte apoptosis index in aFGF-NP + UTMD group reduced to 4.15% and 2.31% respectively, compared with those in the diabetes group (20.5% and 11.3% respectively). Myocardial microvascular density (MCD) in aFGF-NP + UTMD group was up to 35 n/hpf, much higher than that in the diabetes group (14 n/hpf). The diabetes group showed similar results (MCD, CVF and cardiac myocyte apoptosis index) to other aFGF treatment groups (free aFGF +/- UTMD or aFGF-NP). Indexes from transthoracic echocardiography and hemodynamic evaluation also proved the same conclusion. These results confirmed that the abnormalities including diastolic dysfunctions, myocardial fibrosis and metabolic could be suppressed by the different extents of twice weekly aFGF treatments for 12 consecutive weeks (free aFGF or aFGF-NP +/- UTMD), with the strongest improvements observed in the aFGF-NP + UTMD group. Western blot and immunohistochemical analyses of heart tissue samples further revealed the high efficiency of heart-targeted delivery and effective cardioprotection with this combination approach. Overall, this study has generated supportive data that are critical for the translation of a promising DCM prevention strategy. (C) 2015 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81360195, 81301982, 81571392, 81571696, 81272160]; Zhejiang Provincial Foundation for Health Department [2015ZDA023, 2016139678]; Key support of high level talent innovation and technology project of Wenzhou; Wenzhou Bureau of Science and Technology [Y2014730, Y20140726]; Major Scientific Project of Guangdong Province [2012A080201010]; Science and Technology Program of Guangzhou, China [201508020001]
出版者	ELSEVIER SCIENCE BV
出版地	AMSTERDAM
ISSN	0168-3659
EISSN	1873-4995
卷号	223 页码:11-21
DOI	10.1016/j.jconrel.2015.12.030
页数	41
WOS类目	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
WOS记录号	WOS:000368788900002
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
EI入藏号	20160501881277
EI主题词	Ultrasonic applications
URL	查看原文
PubMed ID	26712588
SCOPUSEID	2-s2.0-84961365673
自科自定义期刊分类	T3(B)类
通讯作者地址	[Li, Xiao-Kun]College of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Pharmaceutical Science
TOP期刊	TOP期刊
引用统计	被引频次：28[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8468
专题	药学院（分析测试中心）_药学系附属第二医院附属第一医院
通讯作者	Li, Xiao-Kun
作者单位	1.College of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,325035,China; 2.Second Affiliated Hospital,Wenzhou Medical University,Wenzhou,325000,China; 3.School of Pharmacy,Temple University,Philadelphia,19140,United States; 4.Department of Ultrasonography,Henan Provincial People's Hospital,People's Hospital,Zhengzhou University,Henan,450003,China; 5.Department of Ultrasonography,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	药学院（分析测试中心）_药学系; 附属第二医院
通讯作者单位	温州医科大学
第一作者的第一单位	药学院（分析测试中心）_药学系
推荐引用方式 GB/T 7714	Zhao, Ying-Zheng,Zhang, Ming,Wong, Ho Lun,et al. Prevent diabetic cardiomyopathy in diabetic rats by combined therapy of aFGF-loaded nanoparticles and ultrasound-targeted microbubble destruction technique[J]. JOURNAL OF CONTROLLED RELEASE,2016,223:11-21.
APA	Zhao, Ying-Zheng., Zhang, Ming., Wong, Ho Lun., Tian, Xin-Qiao., Zheng, Lei., ... & Lu, Cui-Tao. (2016). Prevent diabetic cardiomyopathy in diabetic rats by combined therapy of aFGF-loaded nanoparticles and ultrasound-targeted microbubble destruction technique. JOURNAL OF CONTROLLED RELEASE, 223, 11-21.
MLA	Zhao, Ying-Zheng,et al."Prevent diabetic cardiomyopathy in diabetic rats by combined therapy of aFGF-loaded nanoparticles and ultrasound-targeted microbubble destruction technique".JOURNAL OF CONTROLLED RELEASE 223(2016):11-21.