前列腺素E2对重症胰腺炎大鼠血清肿瘤坏死因子及白介素-6的调控作用

doi:10.3969/j.issn.1007-1954.2001.02.006

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题名	前列腺素E2对重症胰腺炎大鼠血清肿瘤坏死因子及白介素-6的调控作用
其他题名	Down-regulatory effect of prostaglandin E2 on serum tumor necrosis and interleukin-6 in rats with severe acute pancreatitis
作者	蓝升红 ; 周丽萍 ; 屠金夫 ; 陈吉彩 ; 吴淑珍 ; 包其郁
发表日期	2001-06-15
发表期刊	肝胆胰外科杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	胰腺炎肿瘤坏死因子白介素6 前列腺素E类
摘要	目的 :探讨前列腺素E2 (PGE2 )对大鼠重症胰腺炎 (SAP)血清白介素 6 (IL 6 )、肿瘤坏死因子(TNF a)的含量及存活率的影响。方法 :SD大鼠 2 9只随机分三组 ,PGE2 组 (N =9) ,SAP组 (N =11)和生理盐水组 (NS ,N =9)。大鼠的SAP模型用 5 %牛磺去氧胆酸钠诱导成功后 30分钟经门静脉注入PGE2 ,采用ELISA检测血清IL 6与TNF a含量 ,动态观察同组不同时间、不同组同时间的IL 6与TNF a变化。并与未经PGE2保护 (SAP组 )及注入生理盐水 (NS组 )相比较。结果 :30分钟时 ,PGE2 组与SAP组的IL 6、TNF a值相比较差异无显著性 (P =2 .79,P =0 .90 7) ,而与NS组比较有显著差异 (P≤ 0 .0 0 1,P =0 .0 14,P <0 .0 0 0 1)。 0 .5～ 2h时 ,PGE2 组的IL 6与TNF a值明显下降 ,而SAP组上述二值却持续上升 (P <0 .0 0 0 1)。 2h～ 6h时 ,PGE2 组上述二值快速下降 ,但SAP组此二值下降缓慢 (P <0 .0 0 0 1) ,差异显著。PGE2 组在同组不同时间的IL 6和TNF a值比较 ,0 .5～ 2h与 2～ 6h时段均呈明显下降态势 (P <0 .0 0 0 1) ,差异有显著性。存活率比较 :0～ 12h时PGE2 保护组 9只 (占 10 0 % )全部存活 ,SAP组死亡 4只 (4 / 11,占 36 .4% )。 0～ 2 4h时 ,SAP组全部死亡 (11/ 11,占 10 0 % ) ,PGE2 组存活 4只 (4 / 9,占 44 .5 % )
其他摘要	Objective：To evaluate the effect of prostaglandin E2 (PGE2) on serum interleukin-6 (IL-6) and tumor necrosis factor (TNF-a) in rats with severe acute pancreatitis.Methods：29 SD rats were randomized into 3 groups: severe acute pancreatitis (SAP,n=11), treated with PGE2 (n=9), saline (NS,n=9). Rats model with SAP were induced via portal vein by injection of 5% sodium taurocholate. 30 minutes after induction of pancreatitis, serum concentration of IL-6 and TNF-a was measured by specific enzyme linked immu-nosorbent assay (ELISA). The IL-6 and TNF-a value changes were observed in iso-group aniso-time and aniso-group iso-time.Results：At 30 minutes, serum IL-6 levels were not significantly different in both PGE2 and SAP groups (P=2.79,P=0.907),but they were different when compared with those in NS group (P≤0.001,P=0.014,P<0.0001). Between 30 minutes and 2 hours serum IL-6 and TNF-a values descended distinctly in PGE2, but continued to elevate in SAP (P<0.0001). However,from 2 hours to 6 hours,IL-6 and TNF-a values decline rapidly in PGE2 than SAP groups (P<0.0001). In addition, from 30 minutes to 2 hours,2 hours to 6 hours,both IL-6 and TNF-a values continue to descend in iso-group aniso-time in PGE2 group,with significant difference (P<0.0001). The survival rate of nine rats treated with PGE2 was 100% but four of SAP group died in 12 hours (4/11,46.4%). Survival rate was significantly higher in PGE2 group (4/9,44.5%) compare with that of SAP group,in which all died (11/11,100%) after 24 hours (P=0.026).Conclusion：TNF-a and IL-6 my be an important mediator in the evaluation of the systematic manifestation of severe acute pancreatitis. PGE2 may suppress serum concentrations of TNF-a and IL-6,and significantly decrease the mortality in rats.
ISSN	1007-1954
卷号	13 期号:02 页码:67-69
DOI	10.3969/j.issn.1007-1954.2001.02.006
页数	3
收录类别	CNKI ; 万方 ; ISTIC
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引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/82495
专题	附属第二医院_外科基础医学院（机能实验教学中心）附属第一医院眼视光学院（生物医学工程学院）、附属眼视光医院_分子生物学实验室
作者单位	1.温州医学院第一附属医院普外科 2.温州医学院微生物、免疫学教研室 3.温州医学院分子生物学实验室!
推荐引用方式 GB/T 7714	蓝升红,周丽萍,屠金夫,等. 前列腺素E2对重症胰腺炎大鼠血清肿瘤坏死因子及白介素-6的调控作用[J]. 肝胆胰外科杂志,2001,13(02):67-69.
APA	蓝升红, 周丽萍, 屠金夫, 陈吉彩, 吴淑珍, & 包其郁. (2001). 前列腺素E2对重症胰腺炎大鼠血清肿瘤坏死因子及白介素-6的调控作用. 肝胆胰外科杂志, 13(02), 67-69.
MLA	蓝升红,et al."前列腺素E2对重症胰腺炎大鼠血清肿瘤坏死因子及白介素-6的调控作用".肝胆胰外科杂志 13.02(2001):67-69.