A New Cyclooxygenase-2 Inhibitor, (1E,4E)-1,5-Bis(2-bromophenyl)penta-1,4-dien-3-one (GL63) Suppresses Cyclooxygenase-2 Gene Expression in Human Lung Epithelial Cancer Cells: Coupled mRNA Stabilization and Posttranscriptional Inhibition

doi:10.1248/bpb.33.1170

科研成果详情

题名	A New Cyclooxygenase-2 Inhibitor, (1E,4E)-1,5-Bis(2-bromophenyl)penta-1,4-dien-3-one (GL63) Suppresses Cyclooxygenase-2 Gene Expression in Human Lung Epithelial Cancer Cells: Coupled mRNA Stabilization and Posttranscriptional Inhibition
作者	Xiao, Jian1,2; Tan, Yi2,3; Pan, Yunbao 1; Liang, Guang2; Qu, Changju 1; Zhang, Xie 2; Zhang, Yi 2; Li, Xiaokun2; Yang, Huiling 1
发表日期	2010-07
发表期刊	BIOLOGICAL & PHARMACEUTICAL BULLETIN 影响因子和分区
语种	英语
原始文献类型	Article
关键词	curcumin analogue cyclooxygenase-2 human antigen R posttranscription
其他关键词	MONO-CARBONYL ANALOGS ; NF-KAPPA-B ; COX-2 INHIBITORS ; CURCUMIN ANALOGS ; BIOLOGICAL EVALUATION ; IN-VIVO ; ACID ; INVOLVEMENT ; MECHANISMS ; STABILITY
摘要	Lung cancer is a leading cause of morbidity and mortality worldwide. Cyclooxygenase-2 (COX-2) expression is upregulated in lung carcinomas and is considered an attractive therapeutic target. In this study, the effect of curcumin and curcumin analogues on COX-2 expression induced by phorbol 12-myristate 13-acetate (PMA) were investigated. We found that a novel curcumin analogue (GL63) inhibited PMA-induced COX-2 mRNA and protein levels in H460 cells to a greater degree than curcumin. To understand the molecular mechanisms governing COX-2 regulation, the effect on COX-2 mRNA degradation was examined; we found that GL63 significantly decreased COX-2 mRNA stability by reducing cytoplasmic localization and protein abundance of human antigen R (HuR). The 3'-untranslated region (3'-UTR) report gene assay also showed GL63 substantially reduced the 3'-UTR green fluorescent protein values, indicating that the destabilizing effect on COX-2 mRNA may be couple with the posttranscriptional inhibition of COX-2. Taken together, our results provide evidence that the novel curcumin analogue can effectively inhibit PMA-induced COX-2 expression in H460 cells, a mechanism associated with COX-2 mRNA stability and post-transcriptional regulation.
资助项目	National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [30901819]; Science Foundation of Zhejiang Province of ChinaNatural Science Foundation of Zhejiang Province [Y2090668]; Science and Technology Grant of Wenzhou city [Y20090175]; Department of Education Science Grant of Zhejiang province [20070992]; Zhejiang Provincial Extremely Key Subject Building Project
出版者	PHARMACEUTICAL SOC JAPAN
出版地	TOKYO
ISSN	0918-6158
EISSN	0918-6158
卷号	33 期号:7 页码:1170-1175
DOI	10.1248/bpb.33.1170
页数	6
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000279199800016
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	20606309
SCOPUSEID	2-s2.0-77954463609
通讯作者地址	[Li, Xiaokun]Key Laboratory of Biotechnology Pharmaceutical Engineering,Wenzhou Medical College,School of Pharmaceutical Science,China
Scopus学科分类	Pharmacology;Pharmaceutical Science
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8192
专题	药学院（分析测试中心）温州医科大学研究生工作部（研究生院）
通讯作者	Li, Xiaokun
作者单位	1.Department of Pathophysiology,Sun Yat-Sen University,China; 2.Key Laboratory of Biotechnology Pharmaceutical Engineering,Wenzhou Medical College,School of Pharmaceutical Science,China; 3.Department of Pediatrics,University of Louisville,United States
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Xiao, Jian,Tan, Yi,Pan, Yunbao,et al. A New Cyclooxygenase-2 Inhibitor, (1E,4E)-1,5-Bis(2-bromophenyl)penta-1,4-dien-3-one (GL63) Suppresses Cyclooxygenase-2 Gene Expression in Human Lung Epithelial Cancer Cells: Coupled mRNA Stabilization and Posttranscriptional Inhibition[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2010,33(7):1170-1175.
APA	Xiao, Jian., Tan, Yi., Pan, Yunbao., Liang, Guang., Qu, Changju., ... & Yang, Huiling. (2010). A New Cyclooxygenase-2 Inhibitor, (1E,4E)-1,5-Bis(2-bromophenyl)penta-1,4-dien-3-one (GL63) Suppresses Cyclooxygenase-2 Gene Expression in Human Lung Epithelial Cancer Cells: Coupled mRNA Stabilization and Posttranscriptional Inhibition. BIOLOGICAL & PHARMACEUTICAL BULLETIN, 33(7), 1170-1175.
MLA	Xiao, Jian,et al."A New Cyclooxygenase-2 Inhibitor, (1E,4E)-1,5-Bis(2-bromophenyl)penta-1,4-dien-3-one (GL63) Suppresses Cyclooxygenase-2 Gene Expression in Human Lung Epithelial Cancer Cells: Coupled mRNA Stabilization and Posttranscriptional Inhibition".BIOLOGICAL & PHARMACEUTICAL BULLETIN 33.7(2010):1170-1175.