颅脑外伤病人痰液中鲍曼不动杆菌gyrA基因突变与环丙沙星耐药关系研究

doi:10.3969/j.issn.1002-2694.2005.05.009

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题名	颅脑外伤病人痰液中鲍曼不动杆菌gyrA基因突变与环丙沙星耐药关系研究
其他题名	Relationship of the chromosomel gyrA gene mutation and ciprofloxacin resistance of Acinetobacter baumannii isolated from the sputum of patients with craniocerebral injury
作者	诸葛青云 ; 李芙蓉 ; 黄支密
发表日期	2005-05-30
发表期刊	中国人兽共患病杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	鲍曼不动杆菌 gyrA基因突变环丙沙星序列分析
摘要	目的了解分离自颅脑外伤病人痰液中鲍曼不动杆菌gyrA基因突变与环丙沙星耐药性的关系。方法用ATB药敏试验条板微量肉汤法测定27株鲍曼不动杆菌对14种抗菌药物的敏感性,采用聚合酶链反应(PCR)及序列分析的方法分析gyrA基因序列。结果27株菌中除HZ16号菌株对环丙沙星敏感外,其余26株均耐药。序列分析表明HZ16号菌株gyrA基因无突变,其序列已登录GenBank(accessionAY642140),其余26株耐药菌均存在gryA基因突变,导致耐喹诺酮决定区(QRDR)第83位的丝氨酸被亮氨酸取代。其中HZ28号菌株gyrA基因序列也已登录(GenBankAY642141)。结论gyrA基因突变是导致分离自颅脑外伤病人痰液中鲍曼不动杆菌对环丙沙星耐药的主要原因。
其他摘要	The susceptibility of 27 strains of Acenetobacter baumannii isolated from the sputum specimens of patients with craniocerebral injury to 14 antimicrobial agents was determined with micro-dilution testing, and the gene sequences of the chromoso-mal gyrA gene from these bacterial strains were analyzed by PCR and verified by DNA sequencing. It was found that all these 27 strains of A . baumannii were resistant to ciprofloxacine except the HZ16 strain. The result of sequence analysis demonstrated that al-so except the HZ16 strain, all strains showed gyrA gene mutations, and the sequence data indicated that there was no mutation of gyrA gene, in HZ16 strain, whose data had been submitted to GenBank under a accession number of AY642140. The gyrA gene mutations of other 26 drug-resistant strains of A. baumannii led to the substitution of Ser-83 by leucine in quinolone-resistance deter-mining region (QRDR). In addition, the sequence data of gyrA gene in HZ28 strain had also been submitted to GenBank with a ac-cession number of AY642141. It is therefore, concluded that gyrA gene mutation is the main cause for the development of drug-resis-tance to ciprofloxacin in strains of A. baumannii isolated in sputum of patients with craniocerebral injury.
ISSN	1002-2694
卷号	21 期号:05 页码:400-402
DOI	10.3969/j.issn.1002-2694.2005.05.009
页数	3
收录类别	CNKI ; CSCD ; 万方 ; 北大核心 ; PKU ; ISTIC
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:2027787
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/81728
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
作者单位	1.温州医学院茶山校区寄生虫教研室 2.浙南心血管医院 3.解放军第98医院检验科 4.温州市鹿城区高田路8号
推荐引用方式 GB/T 7714	诸葛青云,李芙蓉,黄支密. 颅脑外伤病人痰液中鲍曼不动杆菌gyrA基因突变与环丙沙星耐药关系研究[J]. 中国人兽共患病杂志,2005,21(05):400-402.
APA	诸葛青云, 李芙蓉, & 黄支密. (2005). 颅脑外伤病人痰液中鲍曼不动杆菌gyrA基因突变与环丙沙星耐药关系研究. 中国人兽共患病杂志, 21(05), 400-402.
MLA	诸葛青云,et al."颅脑外伤病人痰液中鲍曼不动杆菌gyrA基因突变与环丙沙星耐药关系研究".中国人兽共患病杂志 21.05(2005):400-402.

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