Central zone of myocardial infarction: a neglected target area for heart cell therapy

doi:10.1111/j.1582-4934.2011.01408.x

科研成果详情

题名	Central zone of myocardial infarction: a neglected target area for heart cell therapy
作者	Jin, Peifeng2,3,4; Wang, Enshi 2,3; Wang, Ye-huan 4,5; Huang, Weicong4; Kuang, Wenan 4; Sun, Chengchao4; Hu, Shengshou 2,3; Zhang, Hao 1,2,3
发表日期	2012-03
发表期刊	JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	myocardial infarction mesenchymal stem cells cell transplantation cell therapy ventricular remodelling apoptosis
其他关键词	MESENCHYMAL STEM-CELLS ; STROMAL CELLS ; TRANSPLANTATION ; SURVIVAL ; REPAIR
摘要	The purpose of this study was to investigate the fate of transplanted cells in the central zone of myocardial infarction (MI), and to clarify the relationship between the injection-site impact and the efficacy of cell therapy. MI was created by coronary ligation in female rats. Three weeks later, 3-million labelled male bone marrow mesenchymal stem cells (BMSCs) were directly injected into the border (BZC group) or central zone (CZC group) of MI area. As a control, culture medium was injected into the same sites. Cell survival was evaluated by quantitative real-time polymerase chain reaction, and apoptosis was assayed with TUNEL and caspase-3 staining. Four weeks after transplantation, heart function and cardiac morphometry were evaluated by echocardiography and Massons Trichrome staining, respectively. Angiogenesis and myogenesis were detected by immunofluorescence staining. After cell transplantation into the border or central zone, there was no cell migration between the different zones of MI. BMSCs in the CZC group exhibited no difference in apoptotic percentage, in the long-term survival, when compared with those in the BZC group. However, they did effectively promote angiogenesis and cellular myogenic differentiation. Although cell delivery in the central zone of MI had no effect on the recovery of heart function compared with the BZC group, the retained BMSCs could still increase the scar thickness, and subsequently exhibit a trend in the reverse remodelling of ventricular dilation. Hence, we concluded that the central zone of MI should not be ignored during cell-based therapy. Multiple site injection (border+central zone) is strongly recommended during the procedure of cell transplantation.
资助项目	National Scientific Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81070099]; Fok Ying Tung Education FoundationFok Ying Tung Education Foundation [121041]; National Scientific Foundation of Zhejiang province [Y2110641]; Natural Science Foundation of Wenzhou, Zhejiang, China [h20090019]
出版者	WILEY
出版地	HOBOKEN
ISSN	1582-1838
EISSN	1582-4934
卷号	16 期号:3 页码:637-648
DOI	10.1111/j.1582-4934.2011.01408.x
页数	12
WOS类目	Cell Biology ; Medicine, Research & Experimental
WOS研究方向	Cell Biology ; Research & Experimental Medicine
WOS记录号	WOS:000300831300021
收录类别	SCIE
PubMed ID	21838808
通讯作者地址	[Zhang, Hao]Chinese Acad Med Sci, Cardiovasc Inst, Dept Cardiac Surg, Res Ctr Cardiac Regenerat Med, 167 Beilishi Rd, Beijing 100037, Peoples R China.
TOP期刊	TOP期刊
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8118
专题	附属第一医院附属第一医院_心胸外科
通讯作者	Zhang, Hao
作者单位	1.Chinese Acad Med Sci, Cardiovasc Inst, Dept Cardiac Surg, Res Ctr Cardiac Regenerat Med, Beijing 100037, Peoples R China; 2.Chinese Acad Med Sci, Dept Surg, Beijing 100037, Peoples R China; 3.Chinese Acad Med Sci, Fu Wai Hosp, Beijing 100037, Peoples R China; 4.Wenzhou Med Coll, Affiliated Hosp 1, Dept Thorac & Cardiovasc Surg, Wenzhou, Peoples R China; 5.1st People Hosp Yueyang, Dept Emergency, Yueyang, Peoples R China
第一作者单位	附属第一医院; 附属第一医院_心胸外科
推荐引用方式 GB/T 7714	Jin, Peifeng,Wang, Enshi,Wang, Ye-huan,et al. Central zone of myocardial infarction: a neglected target area for heart cell therapy[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2012,16(3):637-648.
APA	Jin, Peifeng., Wang, Enshi., Wang, Ye-huan., Huang, Weicong., Kuang, Wenan., ... & Zhang, Hao. (2012). Central zone of myocardial infarction: a neglected target area for heart cell therapy. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 16(3), 637-648.
MLA	Jin, Peifeng,et al."Central zone of myocardial infarction: a neglected target area for heart cell therapy".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 16.3(2012):637-648.