Decellularized periosteum as a potential biologic scaffold for bone tissue engineering

doi:10.1016/j.actbio.2015.02.020

科研成果详情

题名	Decellularized periosteum as a potential biologic scaffold for bone tissue engineering
作者	Chen, Kai 1; Lin, Xianfeng 2; Zhang, Qi 3; Ni, Jinhu 3; Li, Jianmin4; Xiao, Jian 6; Wang, Yang3; Ye, Yiheng1; Chen, Li 5; Jin, Keke3; Chen, Lei 1
发表日期	2015-06
发表期刊	ACTA BIOMATERIALIA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Decellularization Periosteum Extracellular matrix Bone tissue engineering
其他关键词	EXTRACELLULAR-MATRIX ; CELLS ; GRAFT ; DEFECTS ; DIFFERENTIATION ; FRACTURES ; REPAIR ; HEART ; LUNG
摘要	Bone grafting or bone substitute is typically used to bridge a bone defect that has been caused by trauma, tumor resection, pathological degeneration, or congenital deformations. However, bone graft healing and remodeling is always a major concern of orthopedic surgeons. Because the periosteum has a remarkable regenerative capacity and is widely recognized to be essential for the initiation of bone graft healing and remodeling, the present study aimed to produce a rabbit decellularized periosteum (D-periosteum) to be used as a biologic scaffold for future bone tissue engineering. We obtained the D-periosteum by employing a combination of commonly used decellularization processes, which include physical methods as well as chemical and enzymatic solutions. The cellular components were effectively removed, and this removal was demonstrated using current decellularization criteria (H&E staining, DAPI staining, DNA quantification and agarose gel electrophoresis); however, there were no significant alterations of the native extracellular matrix (ECM) properties (collagen, glycosaminoglycan (GAG), microarchitecture and mechanical properties). Periosteum-derived cells (PDCs) could adhere, proliferate and infiltrate into the D-periosteum in vitro. The allogenic D-periosteum was implanted subcutaneously into the backs of rabbits over 28 days to study the biocompatibility in vivo. The D-periosteum did not elicit a severe immunogenic response. In summary, a biologic scaffold composed of ECM from periosteum has been successfully developed. The D-periosteum maintains biocompatibility in vitro and in vivo and, therefore, can provide a naturally compatible scaffold for use in future bone tissue engineering. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [Y207495, LY14H070005]
出版者	ELSEVIER SCI LTD
出版地	OXFORD
ISSN	1742-7061
EISSN	1878-7568
卷号	19 页码:46-55
DOI	10.1016/j.actbio.2015.02.020
页数	43
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS研究方向	Engineering ; Materials Science
WOS记录号	WOS:000354152100006
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
EI入藏号	20215111341168
URL	查看原文
PubMed ID	25725472
SCOPUSEID	2-s2.0-84928424215
通讯作者地址	[Jin, Keke]Department of Pathophysiology,Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Biotechnology;Biomaterials;Biochemistry;Biomedical Engineering;Molecular Biology
引用统计	被引频次：52[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8106
专题	附属第一医院基础医学院（机能实验教学中心）_病理学与病理生理学系药学院（分析测试中心）_生物制药系_生物制药工程
通讯作者	Jin, Keke
作者单位	1.Department of Orthopaedics,First Affiliated Hospital,Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Orthopaedic Surgery,Sir Run Run Shaw Hospital,Zhejiang University,Hangzhou,310016,China; 3.Department of Pathophysiology,Wenzhou Medical University,Wenzhou,325000,China; 4.Department of Pathology,First Affiliated Hospital,Wenzhou Medical University,Wenzhou,325000,China; 5.Faculty of Medicine,Dentistry and Health Sciences,University of Melbourne,Melbourne,3010,Australia; 6.Key Laboratory of Biotechnology Pharmaceutical Engineering,Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院
通讯作者单位	温州医科大学
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Chen, Kai,Lin, Xianfeng,Zhang, Qi,et al. Decellularized periosteum as a potential biologic scaffold for bone tissue engineering[J]. ACTA BIOMATERIALIA,2015,19:46-55.
APA	Chen, Kai., Lin, Xianfeng., Zhang, Qi., Ni, Jinhu., Li, Jianmin., ... & Chen, Lei. (2015). Decellularized periosteum as a potential biologic scaffold for bone tissue engineering. ACTA BIOMATERIALIA, 19, 46-55.
MLA	Chen, Kai,et al."Decellularized periosteum as a potential biologic scaffold for bone tissue engineering".ACTA BIOMATERIALIA 19(2015):46-55.