Analysis and characterization of the genes encoding the Dicer and Argonaute proteins of Schistosoma japonicum

doi:10.1186/1756-3305-3-90

科研成果详情

题名	Analysis and characterization of the genes encoding the Dicer and Argonaute proteins of Schistosoma japonicum
作者	Luo, Rong 1; Xue, Xiangyang1,3; Wang, Zhangxun 1; Sun, Jun 1; Zou, Ying 1; Pan, Weiqing 1,2
发表日期	2010-09-17
发表期刊	PARASITES & VECTORS 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	RNA INTERFERENCE ; DISTINCT ROLES ; MESSENGER-RNA ; FAMILY ; INFECTION ; ASSOCIATION ; ELEGANS ; PIWI
摘要	Background: The Dicer and Argonaute( AGO) proteins within the small RNA regulatory pathways ( SRRPs) play an indispensable role in regulation of gene expression. In this study, we analyzed two genes, Dicer and Argonaute, from Schistosoma japonicum, along with their expression through a combination of bioinformatics and experimental approaches. Results: Our results indicate that one Dicer and four Argonaute genes exist in Schistosoma japonicum, termed SjDicer and SjAGO1, 2, 3, and 4, respectively. SjDicer encodes 2590 amino acid residues that contains 5 conserved domains, including one amino-terminal helicase domain, one PAZ ( Piwi-Argonaut-Zwille) domain, two RNAse III domains, and one dsRNA-binding domain. SjAGO1, 2, and 3 encode 1009, 945, and 904 amino acid residues, respectively, all of which contain PAZ and PIWI domains. In addition, we analyzed the expression profiles of SjDicer and SjAGO1 genes by qRT-PCR in eggs, miracidium, cercariae, schistosomula, and adult worms. Results showed consistent expression of both SjDicer and SjAGO1 in different stages; however, their expression levels were stage-dependent, with the highest being in the miracidium stage. Conclusions: This study provided the sequence of the Dicer and Ago genes of S. japonicum and their expression profiles which are essential for further investigation of functions of miRNA in Schistosoma japonicum.
资助项目	National Basic Research Program (973 Program) in ChinaNational Basic Research Program of China [2007CB513100]
出版者	BMC
出版地	LONDON
ISSN	1756-3305
卷号	3 期号:1
DOI	10.1186/1756-3305-3-90
页数	8
WOS类目	Parasitology ; Tropical Medicine
WOS研究方向	Parasitology ; Tropical Medicine
WOS记录号	WOS:000282501300001
收录类别	SCIE ; SCOPUS
URL	查看原文
Pubmed记录号	20849617
Scopus记录号	2-s2.0-77957827859
通讯作者地址	[Pan, Weiqing]Institute for Infectious Diseases and Vaccine Development,Tongji University School of Medicine,1239 Siping Road,China
scopus学科分类	Parasitology;Infectious Diseases
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8050
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学微生物学
通讯作者	Pan, Weiqing
作者单位	1.Institute for Infectious Diseases and Vaccine Development,Tongji University School of Medicine,1239 Siping Road,China; 2.Department of Pathogenic Biology,Second Military Medical University,800 Xiang Yin Road,China; 3.Department of Microbiology,Wenzhou Medical College,University-town,China
推荐引用方式 GB/T 7714	Luo, Rong,Xue, Xiangyang,Wang, Zhangxun,et al. Analysis and characterization of the genes encoding the Dicer and Argonaute proteins of Schistosoma japonicum[J]. PARASITES & VECTORS,2010,3(1).
APA	Luo, Rong, Xue, Xiangyang, Wang, Zhangxun, Sun, Jun, Zou, Ying, & Pan, Weiqing. (2010). Analysis and characterization of the genes encoding the Dicer and Argonaute proteins of Schistosoma japonicum. PARASITES & VECTORS, 3(1).
MLA	Luo, Rong,et al."Analysis and characterization of the genes encoding the Dicer and Argonaute proteins of Schistosoma japonicum".PARASITES & VECTORS 3.1(2010).