Puerarin Increases Survival and Protects Against Organ Injury by Suppressing NF-kappa B/JNK Signaling in Experimental Sepsis

doi:10.3389/fphar.2020.00560

科研成果详情

题名	Puerarin Increases Survival and Protects Against Organ Injury by Suppressing NF-kappa B/JNK Signaling in Experimental Sepsis
作者	Wang, Lei 1; Liang, Qiao 1; Lin, Anqi 1; Chen, Xiufang2; Wu, Yongzhen 1; Zhang, Bin 3; Zhang, Yu 1; Min, Haiyan 1; Wen, Yanting 1; Song, Shiyu 1; Gao, Qian 1
发表日期	2020-05-07
发表期刊	FRONTIERS IN PHARMACOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	puerarin lipopolysaccharides sepsis cecal ligation and puncture liver injury macrophage
其他关键词	INDUCED LIVER-INJURY ; ACUTE LUNG INJURY ; ANIMAL-MODELS ; EXPRESSION ; TNF ; JNK ; HEPATOPROTECTION ; ACTIVATION ; MANAGEMENT ; MICE
摘要	Puerarin, an isoflavonoid rich in Radix Puerariae, has been reported to be a broadly effective regulator in various biological processes and clinic conditions. However, the role of puerarin in sepsis-induced mortality with multiple-organ injury remains unknown. Herein, we showed that puerarin potently attenuated organ injury and increased survival rate in both lipopolysaccharides (LPS) and cecal ligation and puncture (CLP) induced mouse sepsis models. It greatly suppressed systemic inflammation, determined by the serum levels of proinflammatory factors TNF-alpha, IL-6, IL-1 beta, IL-10, as well as monocyte chemotactic protein-1 (MCP-1) and C-reactive protein (CRP). Flow cytometry analysis indicated that puerarin settled overall inflammation mainly by normalizing expanded macrophages with limited effects on dendritic cells and CD4(+)T cells in the circulation of sepsis mice. In the liver, puerarin inhibited the transcription of inflammatory factor TNF-alpha, IL-6, and IL-1 beta and protected hepatocyte apoptosis in sepsis mouse models. In vitro, puerarin inhibited LPS-induced inflammation in LO2 hepatocytes, prevented TNF-alpha-mediated cell apoptosis and promoted an M2 phenotype revealed by M2 marker IL-10 and Arginase-1 (Arg-1) in LPS challenged Raw 264.7 macrophages, through the inhibition of TLR4/NF-kappa B/JNK pathway. In conclusion, puerarin reduced systemic inflammation and protected organ injury in sepsis mice, thus, it might provide a new modality for a better treatment of sepsis.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81570775]; National Key R&D Program of China [2018YFC2001800]
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	1663-9812
EISSN	1663-9812
卷号	11 页码:560
DOI	10.3389/fphar.2020.00560
页数	11
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000536067200001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	32457606
PMC记录号	PMC7221141
Scopus记录号	2-s2.0-85085217999
通讯作者地址	[Song, Shiyu]Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine,Medical School of Nanjing University,Nanjing,China
scopus学科分类	Pharmacology;Pharmacology (medical)
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7993
专题	检验医学院（生命科学学院、生物学实验教学中心）_生物化学与分子生物学系基础医学院（机能实验教学中心）
通讯作者	Song, Shiyu
作者单位	1.Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine,Medical School of Nanjing University,Nanjing,China; 2.Department of Biochemistry,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,China; 3.Central Laboratory,Nanjing Chest Hospital,Medical School of Southeast University,Nanjing,China
推荐引用方式 GB/T 7714	Wang, Lei,Liang, Qiao,Lin, Anqi,et al. Puerarin Increases Survival and Protects Against Organ Injury by Suppressing NF-kappa B/JNK Signaling in Experimental Sepsis[J]. FRONTIERS IN PHARMACOLOGY,2020,11:560.
APA	Wang, Lei., Liang, Qiao., Lin, Anqi., Chen, Xiufang., Wu, Yongzhen., ... & Gao, Qian. (2020). Puerarin Increases Survival and Protects Against Organ Injury by Suppressing NF-kappa B/JNK Signaling in Experimental Sepsis. FRONTIERS IN PHARMACOLOGY, 11, 560.
MLA	Wang, Lei,et al."Puerarin Increases Survival and Protects Against Organ Injury by Suppressing NF-kappa B/JNK Signaling in Experimental Sepsis".FRONTIERS IN PHARMACOLOGY 11(2020):560.