改良五黄油对体外人表皮干细胞生长的影响

doi:10.3321/j.issn:1673-8225.2008.47.005

科研成果详情

题名	改良五黄油对体外人表皮干细胞生长的影响
其他题名	Proliferating effects of Modified Wuhuang Oil on in vitro cultured human epidermal stem cells
作者	辛国华 1; 罗旭2; 曾元临 1; 周湘桂 1; 万子扬 1
发表日期	2008-11-18
发表期刊	中国组织工程研究与临床康复影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	表皮干细胞五黄油细胞增殖噻唑蓝细胞培养
摘要	背景:烧伤创面外用药物五黄油具有抑菌、促进创面愈合的作用,但长期临床应用发现该制剂在止痛、抑制瘢痕形成等方面疗效不确切,且促进表皮生长作用仍有待加强。目的:观察加入黄芪、粉防己碱的改良五黄油不同药物浓度和药物作用时间对人表皮干细胞体外生长增殖的影响。设计、时间及地点:对比观察实验,于2005-10/2006-10在南昌大学医学院烧伤研究所细胞培养实验室完成。材料:2～12岁患者包皮环切术后的新鲜包皮(患者对实验内容均知情同意,并自愿捐献),改良五黄油工作液及五黄油工作液由南昌大学第一附属医院药剂科提供。方法:将不同质量浓度的改良五黄油(0,0.15,0.20,0.25,0.30g/mL)分别加入体外培养的表皮干细胞中,并于5个不同时相点(2,4,6,8,10d),以五黄油为对照组进行MTT细胞生长增殖检测,观察对细胞增殖影响程度。主要观察指标:利用MTT法在酶标仪上分别测定各组表皮干细胞的增殖率。结果:以0,0.15,0.20,0.25,0.30g/mL质量浓度药物作用人表皮干细胞,其增殖程度与改良五黄油的质量浓度正相关性;改良五黄油在0.25g/mL和0.30g/mL质量浓度,相同作用时间(4,6,8d)对表皮干细胞的增殖率高于五黄油,差异有显著性意义(P<0.01)。结论:在一定的体外作用时间范围内,改良五黄油对表皮干细胞的促增殖能力与药物质量浓度呈正相关;改良五黄油对体外表皮干细胞的增殖效果好于五黄油。
其他摘要	BACKGROUND： Wuhuang oil for exterior use for bum wound has promoting effects on healing against bacteria. However, long-term clinical application demonstrated that the preparation has uncertain outcomes on alleviating pain and inhibiting scarfing, as well as the ability on promoting epidermal growth deserves further augmentation. OBJECTIVE： To investigate effects of Modified Wuhuang oil supplemented with astragalus mongholicus and tetrandrine at different concentrations and action time on the proliferation of the human epidermal stem cells （ESCs） in vitro. DESIGN, TIME AND SETTING： The control observation experiment was conducted at the Cell Culture Laboratory of Burn Institute of Medicine School of Nanchang University from October 2005 to October 2006. MATERIALS： Fresh prepuce from 2-12-year old patients following circumcision were selected for this study. Patients signed the informed consent and voluntarily participated in the study. Modified Wuhuang oil and Wuhuang oil were obtained from Department of Pharmacy, First Affiliated Hospital, Nanchang University. METHODS： In vitro cultured ESCs were exposed to different concentrations of Modified Wuhuang oil solution （0, 0.15, 0.20, 0.25, 0.30 g/mL） and investigated by MTT assay after exposure to Modified Wuhuang oil at 2, 4, 6, 8 and 10 hours. Wuhuang oil served as controls to observe the effects on cell proliferation. MAIN OUTCOME MEASURES： MTT was used to measure the proliferative rate of ESCs in each group with a microplate reader. RESULTS： ESCs were treated with drugs at 0, 0.15, 0.20, 0.25, 0.30 g/mL. The proliferative rate of ESCs was positively correlated with the mass concentration of the Modified Wuhuang oil. At mass concentration of 0.25 g/mL and 0.30 g/mL, the proliferative rate of ESCs was higher at 4, 6 and 8 days using the Modified Wuhuang oil compared to Wuhuang oil （P 〈 0,01）. CONCLUSION： During a certain in vitro action time, the promoting proliferation ability of Modified Wuhuang oil is positively correlated with drug mass concentration. The in vitro proliferation outcome of ESCs using Modified Wuhuang oil is better compared to Wuhuang oil.
资助项目	江西省科技厅科技项目资助课题（000486）:改良五黄油在Ⅱ度烧伤创面的应用研究~~
ISSN	1673-8225
卷号	12 期号:47 页码:9231-9234
DOI	10.3321/j.issn:1673-8225.2008.47.005
页数	4
收录类别	CNKI ; CSCD ; 万方 ; 北大核心 ; PKU ; ISTIC
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:3482027
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/79537
专题	附属第一医院_烧伤科
作者单位	1.南昌大学第一附属医院烧伤科; 2.温州医学院第一附属医院烧伤科
推荐引用方式 GB/T 7714	辛国华,罗旭,曾元临,等. 改良五黄油对体外人表皮干细胞生长的影响[J]. 中国组织工程研究与临床康复,2008,12(47):9231-9234.
APA	辛国华, 罗旭, 曾元临, 周湘桂, & 万子扬. (2008). 改良五黄油对体外人表皮干细胞生长的影响. 中国组织工程研究与临床康复, 12(47), 9231-9234.
MLA	辛国华,et al."改良五黄油对体外人表皮干细胞生长的影响".中国组织工程研究与临床康复 12.47(2008):9231-9234.