Postconditioning attenuates renal ischemia-reperfusion injury by mobilization of stem cells

doi:10.1007/s40620-015-0171-7

科研成果详情

题名	Postconditioning attenuates renal ischemia-reperfusion injury by mobilization of stem cells
作者	Tan, Xiaohua1; Yin, Ruili 2; Chen, Yan 3; Gao, Dan 3; Zhang, Xiuying 2
发表日期	2015-06
发表期刊	JOURNAL OF NEPHROLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Postconditioning Stem cells Reactive oxygen species
其他关键词	ACUTE KIDNEY INJURY ; HYPOXIA-INDUCIBLE FACTOR ; PROGENITOR CELLS ; BONE ; FAILURE ; REPAIR ; SDF-1 ; REGENERATION ; ACTIVATION ; MECHANISMS
摘要	Background We recently showed that reactive oxygen species (ROS) and mitochondrial DNA damage and deletions were attenuated by postconditioning (POC). It is not known, however, whether a population of progenitor cells is recruited by POC and is responsible for repair of renal tubular epithelial cells after ischemic injury. Methods The model of renal POC was induced by 45 min clamping of the left renal artery and right nephrectomy followed by 7 min of short-time full reperfusion and 3 cycles of 30 s ischemia and 30 s reperfusion. The lymphocyte compartment of peripheral blood was evaluated by fluorescence-activated cell sorting (FACS) to determine expression of the bone marrow-derived progenitor cell markers CXC-chemokine receptor 4 (CXCR4), c-Kit, and CD34, at 12 h, 1 day and 3 days post-ischemia. Serum and kidney tissue were collected for analysis at 3 and 7 days post-ischemia. Results Renal functional and structural recovery was markedly improved by POC, which increased the number of CXCR4(+) and CD34(+) stem cells in peripheral blood and kidney tissue. Inhibition of ROS burst by POC was likely associated with increased hypoxia-inducible factor-1 expression, which may further promote stromal cell-derived factor 1 (SDF-1) expression. Conclusions The mechanisms of stem cell recruitment to the injured foci mobilized by POC appear to be mediated by moderate oxidative stress, which may lead to increased SDF-1 expression.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81470864]; Program of New Century Excellent Talents of Ministry of Education in ChinaProgram for New Century Excellent Talents in University (NCET) [NCET-10-0448]
出版者	SPRINGER HEIDELBERG
出版地	HEIDELBERG
ISSN	1121-8428
EISSN	1724-6059
卷号	28 期号:3 页码:289-298
DOI	10.1007/s40620-015-0171-7
页数	10
WOS类目	Urology & Nephrology
WOS研究方向	Urology & Nephrology
WOS记录号	WOS:000354892900004
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25663348
SCOPUSEID	2-s2.0-84930205231
通讯作者地址	[Zhang, Xiuying]Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University,You An Men Wai,Beijing,100069,China
Scopus学科分类	Nephrology
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7699
专题	药学院（分析测试中心）药学院（分析测试中心）_分子药理研究与教学中心
通讯作者	Zhang, Xiuying
作者单位	1.Key Laboratory of Biotechnology and Pharmaceutical Engineering, Molecular Pharmacology Research Center, School of Pharmacy, Wenzhou Medical University,Wenzhou,China; 2.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University,You An Men Wai,Beijing,100069,China; 3.Department of Pathology, Norman Bethune School of Medicine, Jilin University,Changchun,China
第一作者单位	分子药理研究与教学中心
第一作者的第一单位	分子药理研究与教学中心
推荐引用方式 GB/T 7714	Tan, Xiaohua,Yin, Ruili,Chen, Yan,et al. Postconditioning attenuates renal ischemia-reperfusion injury by mobilization of stem cells[J]. JOURNAL OF NEPHROLOGY,2015,28(3):289-298.
APA	Tan, Xiaohua, Yin, Ruili, Chen, Yan, Gao, Dan, & Zhang, Xiuying. (2015). Postconditioning attenuates renal ischemia-reperfusion injury by mobilization of stem cells. JOURNAL OF NEPHROLOGY, 28(3), 289-298.
MLA	Tan, Xiaohua,et al."Postconditioning attenuates renal ischemia-reperfusion injury by mobilization of stem cells".JOURNAL OF NEPHROLOGY 28.3(2015):289-298.