MicroRNA-29c Acting on FOS Plays a Significant Role in Nonalcoholic Steatohepatitis Through the Interleukin-17 Signaling Pathway

doi:10.3389/fphys.2021.597449

科研成果详情

题名	MicroRNA-29c Acting on FOS Plays a Significant Role in Nonalcoholic Steatohepatitis Through the Interleukin-17 Signaling Pathway
作者	Cai, Chao1; Chen, Da-Zhi 2; Tu, Han-Xiao 1; Chen, Wen-Kai 1; Ge, Li-Chao 1; Fu, Tian-Tian 3; Tao, Ying 1; Ye, Sha-Sha1; Li, Ji 1; Lin, Zhuo1; Wang, Xiao-Dong 1; Xu, Lan-Man1,4,5; Chen, Yong-Ping1
发表日期	2021-04-01
发表期刊	FRONTIERS IN PHYSIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	FOS NASH micro array Th17 Treg cells bioinformatics
摘要	Nonalcoholic fatty liver disease is the most common hepatic disease in western countries and is even more ubiquitous in Asian countries. Our study determined that TH17/Treg cells were imbalanced in animal models. Based on our interest in the mechanism underlying TH17/Treg cell imbalance in nonalcoholic fatty liver mice, we conducted a joint bioinformatics analysis to further investigate this process. Common gene sequencing analysis was based on one trial from one sequencing platform, where gene expression analysis and enrichment analysis were the only analyses performed. We compared different sequencing results from different trials performed using different sequencing platforms, and we utilized the intersection of these analytical results to perform joint analysis. We used a bioinformatics analysis method to perform enrichment analysis and map interaction network analysis and predict potential microRNA sites. Animal experiments were also designed to validate the results of the data analysis based on quantitative polymerase chain reaction (qPCR) and western blotting. Our results revealed 8 coexisting differentially expressed genes (DEGs) and 7 hinge genes. The identified DEGs may influence nonalcoholic steatosis hepatitis through the interleukin-17 pathway. We found that microRNA-29c interacts with FOS and IGFBP1. Polymerase chain reaction analyses revealed both FOS and microRNA-29c expression in NASH mice, and western blot analyses indicated the same trend with regard to FOS protein levels. Based on these results, we suggest that microRNA-29c acts on FOS via the interleukin-17 signaling pathway to regulate TH17/Treg cells in NASH patients.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81570514, 81770585, 81600466]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY15H030017, LQ15H030006]; National Science and Technology Major Project
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	1664-042X
卷号	12 页码:597449
DOI	10.3389/fphys.2021.597449
页数	12
WOS类目	Physiology
WOS研究方向	Physiology
WOS记录号	WOS:000644561300001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	33927635
PMC记录号	PMC8078210
SCOPUSEID	2-s2.0-85104993911
通讯作者地址	[Xu, Lan-Man]Department of Infectious Disease,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Disease,Hepatology Institute of Wenzhou Medical University,Wenzhou,China ; [Chen, Yong-Ping]Department of Infectious Disease,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Disease,Hepatology Institute of Wenzhou Medical University,Wenzhou,China ; [Xu, Lan-Man]Department of Infectious Diseases and Liver Diseases,Ningbo Medical Centre Lihuili Hospital,Affiliated Lihuili Hospital of Ningbo University,Ningbo,China
Scopus学科分类	Physiology;Physiology (medical)
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7587
专题	第一临床医学院（信息与工程学院）、附属第一医院_精准医学中心实验室附属第一医院
通讯作者	Xu, Lan-Man; Chen, Yong-Ping
作者单位	1.Department of Infectious Disease,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Disease,Hepatology Institute of Wenzhou Medical University,Wenzhou,China; 2.Department of Gastroenterology,The First Hospital of Peking University,Beijing,China; 3.School of Wenzhou Medical University,Wenzhou,China; 4.Department of Infectious Diseases and Liver Diseases,Ningbo Medical Centre Lihuili Hospital,Affiliated Lihuili Hospital of Ningbo University,Ningbo,China; 5.Institute of Innovation for Combined Medicine and Engineering,Ningbo,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Cai, Chao,Chen, Da-Zhi,Tu, Han-Xiao,et al. MicroRNA-29c Acting on FOS Plays a Significant Role in Nonalcoholic Steatohepatitis Through the Interleukin-17 Signaling Pathway[J]. FRONTIERS IN PHYSIOLOGY,2021,12:597449.
APA	Cai, Chao., Chen, Da-Zhi., Tu, Han-Xiao., Chen, Wen-Kai., Ge, Li-Chao., ... & Chen, Yong-Ping. (2021). MicroRNA-29c Acting on FOS Plays a Significant Role in Nonalcoholic Steatohepatitis Through the Interleukin-17 Signaling Pathway. FRONTIERS IN PHYSIOLOGY, 12, 597449.
MLA	Cai, Chao,et al."MicroRNA-29c Acting on FOS Plays a Significant Role in Nonalcoholic Steatohepatitis Through the Interleukin-17 Signaling Pathway".FRONTIERS IN PHYSIOLOGY 12(2021):597449.