天麻素对终末糖基化产物诱导神经小胶质细胞炎症因子表达的影响

科研成果详情

题名	天麻素对终末糖基化产物诱导神经小胶质细胞炎症因子表达的影响
其他题名	Effects of gastrodine on expression of inflammatory cytokines in nerve microglia cell induced by advanced glycation end products
作者	张媛元 1; 毛瑞阳2; 杜晓红2; 刘毅3
发表日期	2011-02-12
发表期刊	中草药影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	天麻素终末糖基化产物神经小胶质细胞白细胞介素1β 白细胞介素6
摘要	目的观察终末糖基化产物(AGEs)对小鼠神经小胶质细胞BV-2炎症因子白细胞介素(IL-1β、IL-6)表达的影响及天麻素的干预效应。方法将体外培养的小鼠小胶质细胞分成对照组、AGEs组、天麻素(12.5、25、50、100 mg/L)组,培养18 h,观察细胞形态变化,酶联免疫吸附法(ELISA)测定培养上清中IL-1βI、l-6水平,逆转录聚合酶链反应(RT-PCR)方法测定IL-1β、IL-6 mRNA表达。结果倒置显微镜下观察,对照组小胶质细胞多为静止期,突起较少,AGEs组多数细胞突起较多,呈阿米巴样,以AGEs质量浓度为300 mg/L时最明显,天麻素干预组细胞突起较AGEs组有所减少,其中以天麻素50 mg/L组最少。与对照组相比,AGEs组细胞培养上清液中IL-1β、IL-6水平,以及细胞内IL-1β、IL-6 mRNA表达明显增加,具有显著差异(P<0.05);与AGEs组相比,天麻素组细胞培养上清液中IL-1β、IL-6水平,以及细胞IL-1β、IL-6 mRNA表达均有所降低,其中天麻素25、50、100 mg/L组差异显著(P<0.05),以天麻素50 mg/L组降低最显著(P<0.01)。结论 AGEs能够诱发神经小胶质细胞产生IL-1β、IL-6,天麻素能部分抑制AGEs对小胶质细胞的诱导作用。
其他摘要	Objective To investigate the effects of gastrodine(GAS)on the expression of IL-1βand IL-6 in nerve microglia BV-2 cell of mice induced by different concentrations of advanced glycation end products(AGEs)and GAS intervention.Methods The cultured BV-2 cells of mice were divided into AGEs group,GAS(12.5,25,50,and 100 mg/L)groups,which were cultured for 18 h to observe the changes of cell morphology.The levels of IL-1βand IL-6 in the cell culture supernate were determined by enzyme linked immunosorbent assay(ELISA)and the expressions of IL-1βand mRNA were analysed by reverse transcriptase polymerase chain reaction(RT-PCR).Results Observed under an inverted microscope,microglia is mostly quiescent and less protruding in control group;Most cell processes were more and amoeba-like in AGEs group,which was most obviously at the concentration of 300 mg/L. Cell processes in GAS groups reduced less than those in AGEs groups,of which the least was in GAS group at concentration of 50 mg/L.Compared with the control group,the levels of IL-1βand IL-6 in supernatants of cell culture in AGEs group and the expressions of IL-1βand IL-6 mRNA increased with significant difference(P<0.05).Compared with AGEs groups,the levels of IL-1βand IL-6 in supernatants of cell culture in AGEs group and the expressions of IL-1βand IL-6 mRNA obviously decreased,there were significant differences in AGE groups at the concentrations of 25,50,and 100 mg/L(P<0.05),among which the decrease was most significant in the AGE group of 50 mg/L(P<0.01).Conclusion AGEs could induce that nerve microglia produce IL-1βand IL-6 and GAS could partly inhibit the induction of AGEs on microglia
资助项目	温州市科技局基金资助课题（Y20070010）
ISSN	0253-2670
卷号	42 期号:02 页码:330-334
页数	5
收录类别	CNKI ; 万方 ; 北大核心 ; PKU ; CSCD ; ISTIC
学科领域	医药、卫生 ; 中国医学
URL	查看原文
CSCD记录号	CSCD:4126336
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/75546
专题	附属第一医院_肾内科附属第一医院附属第一医院_干部保健科
作者单位	1.温州市平阳县第二人民医院肾内科; 2.温州医学院第一附属医院干部科; 3.温州医学院第一附属医院肾内科
推荐引用方式 GB/T 7714	张媛元,毛瑞阳,杜晓红,等. 天麻素对终末糖基化产物诱导神经小胶质细胞炎症因子表达的影响[J]. 中草药,2011,42(02):330-334.
APA	张媛元, 毛瑞阳, 杜晓红, & 刘毅. (2011). 天麻素对终末糖基化产物诱导神经小胶质细胞炎症因子表达的影响. 中草药, 42(02), 330-334.
MLA	张媛元,et al."天麻素对终末糖基化产物诱导神经小胶质细胞炎症因子表达的影响".中草药 42.02(2011):330-334.