3D printed beta-TCP scaffold with sphingosine 1-phosphate coating promotes osteogenesis and inhibits inflammation

doi:10.1016/j.bbrc.2019.03.132

科研成果详情

题名	3D printed beta-TCP scaffold with sphingosine 1-phosphate coating promotes osteogenesis and inhibits inflammation
作者	Cao, Yuxue 1; Xiao, Lan 1,2; Cao, Yanfan 3; Nanda, Ashwin 1; Xu, Chun 1,3; Ye, Qingsong1,3
发表日期	2019-05-14
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Sphingosine 1-phosphate beta-Tricalcium phosphate lmmunoregulation Bone regeneration
其他关键词	SPHINGOSINE-1-PHOSPHATE ; RECEPTORS ; ACTIVATION ; S1P
摘要	Traditional treatments for bone repair with allografts and autografts are limited by the source of bone substitutes. Bone tissue engineering via a cell-based bone tissue scaffold is a new strategy for treatment against large bone defects with many advantages, such as the accessibility of biomaterials, good biocompatibility and osteoconductivity; however, the inflammatory immune response is still an issue that impacts osteogenesis. Sphingosine 1-phosphate (SIP) is a cell-derived sphingolipid that can mediate cell proliferation, immunoregulation and bone regeneration. We hypothesised that coating SIP on a beta-Tricalcium phosphate (beta-TCP) scaffold could regulate the immune response and increase osteogenesis. We tested the immunoregulation capability on macrophages and the osteogenic capability on rat bone marrow stromal cells of the coated scaffolds, which showed good biocompatibility. Additionally, the coated scaffolds exhibited dose-dependent inhibition of inflammatory-related gene expression. A high concentration of SIP (0.5 mu M) upregulated osteogenic-related gene expression of OPN, OCN and RUNX2, which also significantly increased the alkaline phosphatase activity, as compared with the control group. In conclusion, SIP coated beta-TCP scaffold could inhibit inflammation and promote bone regeneration. (C) 2019 Elsevier Inc. All rights reserved.
资助项目	National Health & Medical Research Council of Australia Early Career Fellowship; UQ early career research grant [2016003828]; Australian Microscopy and Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, the University of Queensland
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地	SAN DIEGO
ISSN	0006-291X
EISSN	1090-2104
卷号	512 期号:4 页码:889-895
DOI	10.1016/j.bbrc.2019.03.132
页数	7
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000468258500039
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	30929923
SCOPUSEID	2-s2.0-85064497266
通讯作者地址	[Xu, Chun]School of Dentistry,The University of Queensland,Brisbane,4006,Australia
Scopus学科分类	Biophysics;Biochemistry;Molecular Biology;Cell Biology
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7457
专题	口腔医学院、附属口腔医院_干细胞与组织工程研究所
通讯作者	Xu, Chun
作者单位	1.School of Dentistry,The University of Queensland,Brisbane,4006,Australia; 2.Institute of Health and Biomedical Innovation,Queensland University of Technology,Kelvin Grove Campus,Brisbane,4006,Australia; 3.WMU-UQ Group for Regenerative Medicine,School of Stomatology,Wenzhou Medical University,China
推荐引用方式 GB/T 7714	Cao, Yuxue,Xiao, Lan,Cao, Yanfan,et al. 3D printed beta-TCP scaffold with sphingosine 1-phosphate coating promotes osteogenesis and inhibits inflammation[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,512(4):889-895.
APA	Cao, Yuxue, Xiao, Lan, Cao, Yanfan, Nanda, Ashwin, Xu, Chun, & Ye, Qingsong. (2019). 3D printed beta-TCP scaffold with sphingosine 1-phosphate coating promotes osteogenesis and inhibits inflammation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 512(4), 889-895.
MLA	Cao, Yuxue,et al."3D printed beta-TCP scaffold with sphingosine 1-phosphate coating promotes osteogenesis and inhibits inflammation".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 512.4(2019):889-895.