CircNr1h4 regulates the pathological process of renal injury in salt-sensitive hypertensive mice by targeting miR-155-5p

doi:10.1111/jcmm.14863

科研成果详情

题名	CircNr1h4 regulates the pathological process of renal injury in salt-sensitive hypertensive mice by targeting miR-155-5p
作者	Lu, Chaosheng1; Chen, Bicheng1; Chen, Congcong 1,2; Li, Haiyan 1; Wang, Dan 1; Tan, Yi3,4; Weng, Huachun1
发表日期	2020-01
发表期刊	JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	circular RNAs hypertension miRNAs renal injury
其他关键词	ISCHEMIA-REPERFUSION INJURY ; CIRCULAR RNAS ; PROFILING REVEALS ; BLOOD-PRESSURE ; MICRORNA ; KIDNEY ; EXPRESSION ; DISEASE ; TRANSCRIPTOME ; DEFICIENCY
摘要	Circular RNAs are a class of widespread and diverse endogenous RNAs that may regulate gene expression in various diseases, but their regulation and function in hypertensive renal injury remain unclear. In this study, we generated ribosomal-depleted RNA sequencing data from normal mouse kidneys and from injured mouse kidneys induced by deoxycorticosterone acetate-salt hypertension and identified at least 4900 circRNA candidates. A total of 124 of these circRNAs were differentially expressed between the normal and injured kidneys. Furthermore, we characterized one abundant circRNA, termed circNr1h4, which is derived from the Nr1h4 gene and significantly down-regulated in the injured kidneys. RNA sequencing data and qPCR analysis also showed many microRNAs and mRNAs, including miR-155-5p and fatty acid reductase 1 (Far1), were differentially expressed between the normal and injured kidney and related to circNr1h4. In vitro, the silencing of circNr1h4 or overexpression of miR-155-5p significantly decreased Far1 levels and increased reactive oxygen species. Mechanistic investigations indicated that circNr1h4 acts as a competing endogenous RNA for miR-155-5p, leading to regulation of its target gene Far1. Our study provides novel insight into the molecular mechanisms underlying kidney injury in hypertension, which will be required to develop therapeutic strategies of targeting circRNAs for hypertensive kidney injury.
资助项目	Natural Science Foundation of Zhejiang ChinaNatural Science Foundation of Zhejiang Province [LY19H050001]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670784]
出版者	WILEY
出版地	HOBOKEN
ISSN	1582-1838
EISSN	1582-4934
卷号	24 期号:2 页码:1700-1712
DOI	10.1111/jcmm.14863
页数	13
WOS类目	Cell Biology ; Medicine, Research & Experimental
WOS研究方向	Cell Biology ; Research & Experimental Medicine
WOS记录号	WOS:000499147900001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	31782248
PMC记录号	PMC6991678
SCOPUSEID	2-s2.0-85075714276
通讯作者地址	[Weng, Huachun]Department of Clinical Research & Department of Pediatrics,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Molecular Medicine;Cell Biology
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7450
专题	附属第一医院药学院（分析测试中心）_药学系
通讯作者	Weng, Huachun
作者单位	1.Department of Clinical Research & Department of Pediatrics,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Pharmacy,Jinhua Central Hospital,Jinhua,China; 3.Department of Pharmaceutical Sciences,Wenzhou Medical University,Chashan University-town,Wenzhou,China; 4.Departments of Pediatrics,Pharmacology and Toxicology,Pediatric Research Institute,University of Louisville,Louisville,United States
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Lu, Chaosheng,Chen, Bicheng,Chen, Congcong,et al. CircNr1h4 regulates the pathological process of renal injury in salt-sensitive hypertensive mice by targeting miR-155-5p[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2020,24(2):1700-1712.
APA	Lu, Chaosheng., Chen, Bicheng., Chen, Congcong., Li, Haiyan., Wang, Dan., ... & Weng, Huachun. (2020). CircNr1h4 regulates the pathological process of renal injury in salt-sensitive hypertensive mice by targeting miR-155-5p. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 24(2), 1700-1712.
MLA	Lu, Chaosheng,et al."CircNr1h4 regulates the pathological process of renal injury in salt-sensitive hypertensive mice by targeting miR-155-5p".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 24.2(2020):1700-1712.