Chromone Derivatives CM3a Potently Eradicate Staphylococcus aureus Biofilms by Inhibiting Cell Adherence

doi:10.2147/IDR.S301483

科研成果详情

题名	Chromone Derivatives CM3a Potently Eradicate Staphylococcus aureus Biofilms by Inhibiting Cell Adherence
作者	Zhan, Qing 1; Xu, Yanlei 1; Zhan, Lingling2; Wang, Bingjie 3; Guo, Yinjuan 3,4; Wu, Xiaocui 3,4; Ai, Wenxiu 5; Song, Zengqiang6; Yu, Fangyou 3,4
发表日期	2021
发表期刊	INFECTION AND DRUG RESISTANCE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	chromone derivative maleimide Staphylococcus aureus biofilm eradicate
其他关键词	PRIVILEGED SCAFFOLD ; DRUG DISCOVERY ; INFECTIONS
摘要	Introduction: The ability of Staphylococcus aureus to form biofilms is associated with high mortality and treatment costs. Established biofilms cannot be eradicated by many conventional antibiotics due to the development of antibiotic tolerance by S. aureus. Here we report the synthesis and biological characterization of novel small-molecule compounds with antibiofilm activity. Chromone 5-maleimide substitution compounds (CM3a) showed favorable antibacterial activity against S. aureus. Methods: CM3A with antibacterial activity was synthesized and screened. The minimum inhibitory concentration (MIC) of CM3a were determined by the broth microdilution method. Biofilm eradication assay and colony count methods were used to investigate the effect of CM3a on S. aureus biofilm disruption and killing. Changes in biofilm architecture when subjected to CM3a, were visualized using confocal laser scanning microscopy (CLSM). CCK-8 assay and survival rate of Galleria mellonella larvae were used to test the toxicity of CM3a. Results: The minimum inhibitory concentration (MIC) of CM3a against S. aureus was about 26.4 mu M. Biofilm staining and laser scanning confocal microscopy analysis showed that CM3a eradicated S. aureus biofilms by reducing the viability of the constituent bacterial cells. On the other hand, CM3a showed negligible toxicity against mouse alveolar epithelial cells and Galleria mellonella larvae. Conclusion: Chromone derivatives CM3a has therapeutic potential as a safe and effective compound for the treatment of S. aureus infection.
资助项目	Natural Science Fund of ChinaNational Natural Science Foundation of China (NSFC) [81871704]
出版者	DOVE MEDICAL PRESS LTD
出版地	ALBANY
ISSN	1178-6973
卷号	14 页码:979-986
DOI	10.2147/IDR.S301483
页数	8
WOS类目	Infectious Diseases ; Pharmacology & Pharmacy
WOS研究方向	Infectious Diseases ; Pharmacology & Pharmacy
WOS记录号	WOS:000630395400013
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	33737820
PMC记录号	PMC7961208
SCOPUSEID	2-s2.0-85103085846
通讯作者地址	[Song, Zengqiang]School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,325000,China ; [Yu, Fangyou]Department of Clinical Laboratory Medicine,Shanghai Pulmonary Hospital,Tongji University School of Medicine,Shanghai,200082,China ; [Yu, Fangyou]Shanghai Key Laboratory of Tuberculosis,Shanghai Pulmonary Hospital,Tongji University School of Medicine,Shanghai,200082,China
Scopus学科分类	Pharmacology;Infectious Diseases;Pharmacology (medical)
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7437
专题	检验医学院（生命科学学院、生物学实验教学中心）药学院（分析测试中心）附属第一医院_呼吸内科
通讯作者	Song, Zengqiang; Yu, Fangyou
作者单位	1.Jiangxi Provincial Key Laboratory of Preventive Medicine,School of Public Health,Nanchang University,Nanchang,330006,China; 2.Department of Laboratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Clinical Laboratory Medicine,Shanghai Pulmonary Hospital,Tongji University School of Medicine,Shanghai,200082,China; 4.Shanghai Key Laboratory of Tuberculosis,Shanghai Pulmonary Hospital,Tongji University School of Medicine,Shanghai,200082,China; 5.Department of Respiratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 6.School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,325000,China
通讯作者单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Zhan, Qing,Xu, Yanlei,Zhan, Lingling,et al. Chromone Derivatives CM3a Potently Eradicate Staphylococcus aureus Biofilms by Inhibiting Cell Adherence[J]. INFECTION AND DRUG RESISTANCE,2021,14:979-986.
APA	Zhan, Qing., Xu, Yanlei., Zhan, Lingling., Wang, Bingjie., Guo, Yinjuan., ... & Yu, Fangyou. (2021). Chromone Derivatives CM3a Potently Eradicate Staphylococcus aureus Biofilms by Inhibiting Cell Adherence. INFECTION AND DRUG RESISTANCE, 14, 979-986.
MLA	Zhan, Qing,et al."Chromone Derivatives CM3a Potently Eradicate Staphylococcus aureus Biofilms by Inhibiting Cell Adherence".INFECTION AND DRUG RESISTANCE 14(2021):979-986.