Characterization of a late gene, ORF75 from Bombyx mori nucleopolyhedrovirus

doi:10.1007/s11033-010-0341-6

科研成果详情

题名	Characterization of a late gene, ORF75 from Bombyx mori nucleopolyhedrovirus
作者	Ge, Jun-Qing 1,2; Gao, Guo-Hui3; Xu, Yi-Peng 1; Zhang, Chuan-Xi 1
发表日期	2011-03
发表期刊	MOLECULAR BIOLOGY REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Bombyx mori nucleopolyhedrovirus Bm75 P33 Late gene Nonstructural protein
其他关键词	OCCLUSION-DERIVED VIRUS ; NUCLEAR-POLYHEDROSIS-VIRUS ; SINGLE-NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; STRUCTURAL PROTEIN ; BACULOVIRUS ; EXPRESSION ; APOPTOSIS ; ENVELOPE ; ENCODES ; TRANSCRIPTION
摘要	Open reading frame 75 (Bm-p33) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a homologue of Autographa californica multiple nucleopolyhedrovirus ORF92. The gene is conserved among all baculoviruses that have been completely sequenced to date and is considered to be a baculovirus core set gene. No amino acid mutation was found in Bm-p33 sequences among six BmNPV strains differing in geography, phenotype, or host. The Bm-p33 transcript can be detected as early as 12 h post infection (h p.i.) and remains detectable until 96 h p.i. The Bm-p33 protein was detected in cell lysates from 18 h p.i. through 96 h p.i., and no positive band could be detected in budded viruses (BVs) and occlusion-derived viruses (ODVs) by western blot using anti-Bm-p33 serum. Immunofluorescence microscopy indicated that Bm-p33 accumulated in the nuclear membrane and the intranuclear region, especially near the nuclear membrane of the virus-infected cells. Bm75 RNAi significantly decreased the mRNA level. However, no obvious effects on ODV formation and BV production in BmNPV-infected cells could be detected. Bm-p33 is a BmNPV late gene encoding a nonstructural protein which may function mainly in the nucleus of the infected cells.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30570074]
出版者	SPRINGER
出版地	DORDRECHT
ISSN	0301-4851
EISSN	1573-4978
卷号	38 期号:3 页码:2141-2149
DOI	10.1007/s11033-010-0341-6
页数	9
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000286941400089
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	20848211
SCOPUSEID	2-s2.0-79951580231
通讯作者地址	[Zhang, Chuan-Xi]Key Laboratory of Molecular Biology of Crop Pathogens and Insects,Zhejiang University,Ministry of Agriculture,Kaixuan Road 268,China
Scopus学科分类	Molecular Biology;Genetics
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7224
专题	温州医科大学
通讯作者	Zhang, Chuan-Xi
作者单位	1.Key Laboratory of Molecular Biology of Crop Pathogens and Insects,Zhejiang University,Ministry of Agriculture,Kaixuan Road 268,China; 2.Institute of Biotechnology,Fujian Academy of Agricultural Sciences,Wusi Road 247,China; 3.School of Life Science,Wenzhou Medical College,China
推荐引用方式 GB/T 7714	Ge, Jun-Qing,Gao, Guo-Hui,Xu, Yi-Peng,et al. Characterization of a late gene, ORF75 from Bombyx mori nucleopolyhedrovirus[J]. MOLECULAR BIOLOGY REPORTS,2011,38(3):2141-2149.
APA	Ge, Jun-Qing, Gao, Guo-Hui, Xu, Yi-Peng, & Zhang, Chuan-Xi. (2011). Characterization of a late gene, ORF75 from Bombyx mori nucleopolyhedrovirus. MOLECULAR BIOLOGY REPORTS, 38(3), 2141-2149.
MLA	Ge, Jun-Qing,et al."Characterization of a late gene, ORF75 from Bombyx mori nucleopolyhedrovirus".MOLECULAR BIOLOGY REPORTS 38.3(2011):2141-2149.