A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs

doi:10.1089/zeb.2016.1263

科研成果详情

题名	A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs
作者	Zhu, Xiao-Yu1,2,3; Liu, Hong-Cui 2; Guo, Sheng-Ya 2; Xia, Bo 2; Song, Ru-Shun 2; Lao, Qiao-Cong 2; Xuan, Yao-Xian 3,4; Li, Chun-Qi1,2,3
发表日期	2016-08
发表期刊	ZEBRAFISH 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	FUFANG XUESHUANTONG CAPSULE ; HEMOSTASIS ; PREVENTION ; RATS ; PHOSPHATIDYLSERINE ; EXTERNALIZATION ; IDENTIFICATION ; OCCLUSION ; TOXICITY ; DISEASE
摘要	Thrombosis is a leading cause of death and the development of effective and safe therapeutic agents for thrombotic diseases has been proven challenging. In this study, taking advantage of the transparency of larval zebrafish, we developed a larval zebrafish thrombosis model for drug screening and efficacy assessment. Zebrafish at 2 dpf (days post fertilization) were treated with phenylhydrazine (PHZ) and a testing drug for 24 h. Tested drugs were administered into the zebrafish either by direct soaking or circulation microinjection. Antithrombotic efficacy was quantitatively evaluated based on our previously patented technology characterized as an image analysis of the heart red blood cells stained with O-dianisidine staining. Zebrafish at 2 dpf treated with PHZ at a concentration of 1.5 mu M for a time period of 24 h were determined as the optimum conditions for the zebrafish thrombosis model development. Induced thrombosis in zebrafish was visually confirmed under a dissecting stereomicroscope and quantified by the image assay. All 6 human antithrombotic drugs (aspirin, clopidogrel, diltiazem hydrochloride injection, xuanshuantong injection, salvianolate injection, and astragalus injection) showed significant preventive and therapeutic effects on zebrafish thrombosis (p < 0.05, p < 0.01, & p < 0.001) in this zebrafish thrombosis model. The larval zebrafish thrombosis model developed and validated in this study could be used for in vivo thrombosis studies and for rapid screening and efficacy assessment of antithrombotic drugs.
资助项目	Zhejiang Provincial Key Science & Technology Project [2014C03009]; National Key Science & Technology Project grant of the 12th 5-year program of China [2011ZX09301-003]
出版者	MARY ANN LIEBERT, INC
出版地	NEW ROCHELLE
ISSN	1545-8547
EISSN	1557-8542
卷号	13 期号:4 页码:335-344
DOI	10.1089/zeb.2016.1263
页数	10
WOS类目	Developmental Biology ; Zoology
WOS研究方向	Developmental Biology ; Zoology
WOS记录号	WOS:000378330600014
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	27333081
SCOPUSEID	2-s2.0-84975840791
通讯作者地址	[Li, Chun-Qi]Hunter Biotechnology,Inc.,No. 88 Jiangling Road,Hangzhou City,310051,China
Scopus学科分类	Animal Science and Zoology;Developmental Biology
引用统计	被引频次：46[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7212
专题	检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
通讯作者	Li, Chun-Qi
作者单位	1.Zhejiang Provincial Key Lab for Technology and Application of Model Organisms,Wenzhou Medical University,Wenzhou,China; 2.Hunter Biotechnology,Inc.,No. 88 Jiangling Road,Hangzhou City,310051,China; 3.Innovation Team of Drug Safety Evaluation,Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals,Hangzhou,China; 4.Center of Safety Evaluation,Zhejiang Academy of Medical Sciences,Hangzhou,China
第一作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Zhu, Xiao-Yu,Liu, Hong-Cui,Guo, Sheng-Ya,et al. A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs[J]. ZEBRAFISH,2016,13(4):335-344.
APA	Zhu, Xiao-Yu., Liu, Hong-Cui., Guo, Sheng-Ya., Xia, Bo., Song, Ru-Shun., ... & Li, Chun-Qi. (2016). A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs. ZEBRAFISH, 13(4), 335-344.
MLA	Zhu, Xiao-Yu,et al."A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs".ZEBRAFISH 13.4(2016):335-344.