DI-3-n-butylphthalide attenuates acute inflammatory activation in rats with spinal cord injury by inhibiting microglial TLR4/NF-kappa B signalling

doi:10.1111/jcmm.13212

科研成果详情

题名	DI-3-n-butylphthalide attenuates acute inflammatory activation in rats with spinal cord injury by inhibiting microglial TLR4/NF-kappa B signalling
作者	He, Zili1,2; Zhou, Yulong1,2; Lin, Li2; Wang, Qingqing 1; Khor, Sinan 3; Mao, Yuqin 2; Li, Jiawei1; Zhen, Zengming 1; Chen, Jian1; Gao, Zhenzhen 2; Wu, Fenzan 4; Zhang, Xie 5; Zhang, Hongyu2; Xu, Hua-Zi1; Wang, Zhouguang 2; Xiao, Jian1,2
发表日期	2017-11
发表期刊	JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	DI-3-n-butylphthalide (NBP) inflammatory microglia spinal cord injury
其他关键词	FOCAL CEREBRAL-ISCHEMIA ; AMYOTROPHIC-LATERAL-SCLEROSIS ; BETA-CELL FUNCTION ; MOUSE MODEL ; C-KIT ; MICE ; SURVIVAL ; DAMAGE ; 3-N-BUTYLPHTHALIDE ; REGENERATION
摘要	In this study, we examined the neuroprotective effects and anti-inflammatory properties of Dl-3-n-butylphthalide (NBP) in Sprague-Dawley (SD) rats following traumatic spinal cord injury (SCI) as well as microglia activation and inflammatory response both in vivo and in vitro. Our results showed that NBP improved the locomotor recovery of SD rats after SCI an significantly diminished the lesion cavity area of the spinal cord, apoptotic activity in neurons, and the number of TUNEL-positive cells at 7 days post-injury. NBP inhibited activation of microglia, diminished the release of inflammatory mediators, and reduced the upregulation of microglial TLR4/NF-kappa B expression at 1 day post-injury. In a co-culture system with BV-2 cells and PC12 cells, NBP significantly reduced the cytotoxicity of BV-2 cells following lipopolysaccharide (LPS) stimulation. In addition, NBP reduced the activation of BV-2 cells, diminished the release of inflammatory mediators, and inhibited microglial TLR4/NF-kappa B expression in BV-2 cells. Our findings demonstrate that NBP may have neuroprotective and anti-inflammatory properties in the treatment of SCI by inhibiting the activation of microglia via TLR4/NF-kappa B signalling.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81601980, 81572237, 81302775, 81572227, 81501953]; Zhejiang Provincial Natural Science FoundationNatural Science Foundation of Zhejiang Province [Y14H170002, Y14H170008, LY17H090017, LQ16H090007]; Technologies Science and technology Program of Zhejiang Provincial [2016C33107]; Ningbo City Natural Science Foundation [2015A610213, 2015A610208]; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [T32GM007288] Funding Source: NIH RePORTER
出版者	WILEY
出版地	HOBOKEN
ISSN	1582-4934
EISSN	1582-4934
卷号	21 期号:11 页码:3010-3022
DOI	10.1111/jcmm.13212
页数	13
WOS类目	Cell Biology ; Medicine, Research & Experimental
WOS研究方向	Cell Biology ; Research & Experimental Medicine
WOS记录号	WOS:000413962100032
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28842949
PMC记录号	PMC5661102
SCOPUSEID	2-s2.0-85032506632
通讯作者地址	[Wang, Zhouguang]Molecular Pharmacology Research Center,School of Pharmaceutical Science,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Molecular Medicine;Cell Biology
TOP期刊	TOP期刊
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7202
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院药学院（分析测试中心）_分子药理研究与教学中心其他_附属慈溪医院（慈溪市人民医院）
通讯作者	Wang, Zhouguang
作者单位	1.Department of Orthopaedics,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou Medical University,Wenzhou,China; 2.Molecular Pharmacology Research Center,School of Pharmaceutical Science,Wenzhou Medical University,Wenzhou,China; 3.Department of Molecular Pharmacology,Albert Einstein College of Medicine,Bronx,United States; 4.Department of Neurosurgery,Affiliated Cixi People's Hospital,Wenzhou Medical University,Ningbo,China; 5.Department of Gastroenterology,Ningbo Medical Treatment Center Li Hui-li Hospital,Ningbo,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 药学院（分析测试中心）; 分子药理研究与教学中心
通讯作者单位	药学院（分析测试中心）; 分子药理研究与教学中心
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	He, Zili,Zhou, Yulong,Lin, Li,et al. DI-3-n-butylphthalide attenuates acute inflammatory activation in rats with spinal cord injury by inhibiting microglial TLR4/NF-kappa B signalling[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2017,21(11):3010-3022.
APA	He, Zili., Zhou, Yulong., Lin, Li., Wang, Qingqing., Khor, Sinan., ... & Xiao, Jian. (2017). DI-3-n-butylphthalide attenuates acute inflammatory activation in rats with spinal cord injury by inhibiting microglial TLR4/NF-kappa B signalling. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 21(11), 3010-3022.
MLA	He, Zili,et al."DI-3-n-butylphthalide attenuates acute inflammatory activation in rats with spinal cord injury by inhibiting microglial TLR4/NF-kappa B signalling".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 21.11(2017):3010-3022.