Cardiovascular protection of nonmitogenic human acidic fibroblast growth factor from oxidative damage in vitro and in vivo

doi:10.1016/j.carpath.2006.11.004

科研成果详情

题名	Cardiovascular protection of nonmitogenic human acidic fibroblast growth factor from oxidative damage in vitro and in vivo
作者	Li, Xiao-Kun1,2,3; Lin, Zhuo-Feng1,2; Li, Yan 2; Hu, Shifeng 1; Tan, Yi1; Huang, Zhifeng1; Meng, Juan 2; Liang, Li-Ming 1; Xiao, Jian1; Qu, Jia3; Cai, Lu1,4,5,6
发表日期	2007-04
发表期刊	CARDIOVASCULAR PATHOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	cardiomyocytes fibroblast growth factor ischemia/reperfusion oxidative damage cardiovascular protection carotid sinus hydrogen peroxide alcohol
其他关键词	ISCHEMIA-REPERFUSION INJURY ; SKELETAL-MUSCLE ; TRANSGENIC MICE ; METALLOTHIONEIN ; RESISTANT ; HEART ; NEUROPROTECTION ; INHIBITION ; MYOCARDIUM ; ETHANOL
摘要	Background: In our previous study, a mutant human acidic fibroblast growth factor without mitogenic action (nonmitogenic human acidic fibroblast growth factor) was created, and its protection from the cytotoxic effect of hydrogen peroxide treatment was confirmed in cultured cardiomyocytes. Methods: The present study was performed to further investigate whether genetically overexpressing nonmitogenic human acidic fibroblast growth factor in cardiomyocytes provides similar protection from the cytotoxic effect of hydrogen peroxide and whether in vivo administration of nonmitogenic human acidic fibroblast growth factor attenuates ischemia/reperfusion-induced cardiac dysfunction and tissue damage and protects the carotid sinus baroreceptor from alcohol-induced damage, as shown by a reduced response of blood pressure to short carotid artery occlusion. Results and conclusions: Cardiomyocytes transfected by nonmitogenic human acidic fibroblast growth factor, with significant increases in the cellular expression and secretion of nonmitogenic human acidic fibroblast growth factor into a culture medium, were resistant to hydrogen-peroxide-induced cytotoxicity, as measured by cell viability. Hearts isolated from rats pretreated with saline, human acidic fibroblast growth factor, or nonmitogenic human acidic fibroblast growth factor for 24 h were subjected to ischemia/reperfusion in the Langendorff system. Ischemia/reperfusion induced cardiac dysfunction in the saline group, but not in the group pretreated with human acidic fibroblast growth factor or nonmitogenic human acidic fibroblast growth factor. Ischemia/reperfusion also caused a release of the cardiac enzyme lactic dehydrogenase into-and an increase in lipid peroxide content in the efflux of-the hearts of saline-treated rats, but not in rats pretreated with human acidic fibroblast growth factor or nonmitogenic human acidic fibroblast growth factor. There was no difference in cardioprotective effects between human acidic fibroblast growth factor and nonmitogenic human acidic fibroblast growth factor. Furthermore, the protective effect of in-vivo-administered nonmitogenic acidic fibroblast growth factor on alcohol-induced damage to the carotid sinus baroreceptor, as shown by the reduced response of blood pressure to short carotid artery occlusion, was also observed. These results suggest that nonmitogenic human acidic fibroblast growth factor, similar to the native human acidic fibroblast growth factor, provides significant cardiovascular protection from oxidative damage in vitro and in vivo. (C) 2007 Elsevier Inc. All rights reserved.
资助项目	National High-tech Research and Development Program[2002AA2Z3318];
出版者	ELSEVIER SCIENCE INC
出版地	NEW YORK
ISSN	1054-8807
卷号	16 期号:2 页码:85-91
DOI	10.1016/j.carpath.2006.11.004
页数	7
WOS类目	Cardiac & Cardiovascular Systems ; Pathology
WOS研究方向	Cardiovascular System & Cardiology ; Pathology
WOS记录号	WOS:000244733400005
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	17317541
SCOPUSEID	2-s2.0-33847030254
通讯作者地址	[Li, Xiao-Kun]Research Institute for Diabetic Complications,School of Pharmaceutical Sciences,Wenzhou Medical College,China
Scopus学科分类	Pathology and Forensic Medicine;Cardiology and Cardiovascular Medicine
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7170
专题	药学院（分析测试中心）眼视光学院（生物医学工程学院）、附属眼视光医院研究生工作部（研究生院）
通讯作者	Li, Xiao-Kun
作者单位	1.Research Institute for Diabetic Complications,School of Pharmaceutical Sciences,Wenzhou Medical College,China; 2.School of Pharmacy and Medicine,Jinan University,China; 3.School of Ophthalmology and Optometry,Wenzhou Medical College,China; 4.Department of Medicine,University of Louisville,United States; 5.Department of Radiation Oncology,University of Louisville,United States; 6.Department of Pharmacology and Toxicology,University of Louisville,United States
第一作者单位	药学院（分析测试中心）; 眼视光学院（生物医学工程学院）、附属眼视光医院
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Li, Xiao-Kun,Lin, Zhuo-Feng,Li, Yan,et al. Cardiovascular protection of nonmitogenic human acidic fibroblast growth factor from oxidative damage in vitro and in vivo[J]. CARDIOVASCULAR PATHOLOGY,2007,16(2):85-91.
APA	Li, Xiao-Kun., Lin, Zhuo-Feng., Li, Yan., Hu, Shifeng., Tan, Yi., ... & Cai, Lu. (2007). Cardiovascular protection of nonmitogenic human acidic fibroblast growth factor from oxidative damage in vitro and in vivo. CARDIOVASCULAR PATHOLOGY, 16(2), 85-91.
MLA	Li, Xiao-Kun,et al."Cardiovascular protection of nonmitogenic human acidic fibroblast growth factor from oxidative damage in vitro and in vivo".CARDIOVASCULAR PATHOLOGY 16.2(2007):85-91.