Subinhibitory Concentrations of Fusidic Acid May Reduce the Virulence of S. aureus by Down-Regulating sarA and saeRS to Reduce Biofilm Formation and alpha-Toxin Expression

doi:10.3389/fmicb.2020.00025

科研成果详情

题名	Subinhibitory Concentrations of Fusidic Acid May Reduce the Virulence of S. aureus by Down-Regulating sarA and saeRS to Reduce Biofilm Formation and alpha-Toxin Expression
作者	Liu, Li 1; Shen, Xiaofei 2; Yu, Jingyi 1; Cao, Xingwei 3; Zhan, Qing 4; Guo, Yinjuan 5,6; Yu, Fangyou 5,6
发表日期	2020-02-14
发表期刊	FRONTIERS IN MICROBIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	subinhibitory concentration fusidic acid biofilm saeRS sarA alpha-toxin
其他关键词	MUREIN HYDROLASE ACTIVITY ; STAPHYLOCOCCUS ; ANTIBIOTICS ; RESISTANCE ; RELEASE ; OPERON
摘要	Staphylococcus aureus is an important pathogen in hospital and community infections. Fusidic acid is particularly effective in treating skin and wound infections caused by staphylococci. The purpose of our study was to clarify the effect of fusidic acid on the biofilm formation and alpha-toxin expression of S. aureus at subinhibitory concentrations [1/64, 1/32, and 1/16 x minimum inhibitory concentration (MIC)]. A total of 504 genes greater than a twofold or less than twofold change in expression of S. aureus effected by subinhibitory concentrations of fusidic acid were found, including 232 up-regulated genes and 272 down-regulated genes, which were determined by transcriptome sequencing. Our results showed subinhibitory concentrations of fusidic acid significantly inhibited the expression of hla, spa, icaA, and cidA at the mRNA level in clinical S. aureus strains tested. And subinhibitory concentrations of fusidic acid can significantly reduce the hemolysis activity and alpha-toxin production of S. aureus. In addition, the subinhibitory concentrations of fusidic acid significantly inhibited biofilm formation, autolysis, cell aggregation, and polysaccharide intercellular adhesin (PIA) production of S. aureus. Moreover, fusidic acid effectively reduces the damage of mouse skin lesion area. Furthermore, fusidic acid reduced the expression of the two-component regulatory system saeRS and staphylococcal accessory gene regulator (sarA). In conclusion, our results suggested that the subinhibitory concentrations of fusidic acid may reduce the virulence of S. aureus by down-regulating sarA and saeRS to reduce biofilm formation and alpha-toxin expression, which will provide a theoretical basis for the clinical treatment of S. aureus infection. This is the first report that fusidic acid has an inhibitory effect on the virulence of S. aureus, and this broadens the clinical application of fusidic acid.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81871704]
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	1664-302X
EISSN	1664-302X
卷号	11 页码:25
DOI	10.3389/fmicb.2020.00025
页数	13
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:000517407400001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	32117092
PMC记录号	PMC7033611
SCOPUSEID	2-s2.0-85083913094
通讯作者地址	[Yu, Fangyou]Department of Clinical Laboratory,Shanghai Pulmonary Hospital,Tongji University School of Medicine,Shanghai,China
Scopus学科分类	Microbiology;Microbiology (medical)
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7124
专题	检验医学院（生命科学学院、生物学实验教学中心）附属第一医院_呼吸内科
通讯作者	Yu, Fangyou
作者单位	1.Department of Laboratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Respiratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.Jiangxi Provincial Key Laboratory of Medicine,Clinical Laboratory of the Second Affiliated Hospital of Nanchang University,Nanchang,China; 4.Jiangxi Provincial Key Laboratory of Preventive Medicine,Nanchang University,Nanchang,China; 5.Department of Clinical Laboratory,Shanghai Pulmonary Hospital,Tongji University School of Medicine,Shanghai,China; 6.Shanghai Key Laboratory of Tuberculosis,Shanghai Pulmonary Hospital,Tongji University School of Medicine,Shanghai,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Liu, Li,Shen, Xiaofei,Yu, Jingyi,et al. Subinhibitory Concentrations of Fusidic Acid May Reduce the Virulence of S. aureus by Down-Regulating sarA and saeRS to Reduce Biofilm Formation and alpha-Toxin Expression[J]. FRONTIERS IN MICROBIOLOGY,2020,11:25.
APA	Liu, Li., Shen, Xiaofei., Yu, Jingyi., Cao, Xingwei., Zhan, Qing., ... & Yu, Fangyou. (2020). Subinhibitory Concentrations of Fusidic Acid May Reduce the Virulence of S. aureus by Down-Regulating sarA and saeRS to Reduce Biofilm Formation and alpha-Toxin Expression. FRONTIERS IN MICROBIOLOGY, 11, 25.
MLA	Liu, Li,et al."Subinhibitory Concentrations of Fusidic Acid May Reduce the Virulence of S. aureus by Down-Regulating sarA and saeRS to Reduce Biofilm Formation and alpha-Toxin Expression".FRONTIERS IN MICROBIOLOGY 11(2020):25.