Identification and Characterization of Novel MicroRNAs from Schistosoma japonicum

doi:10.1371/journal.pone.0004034

科研成果详情

题名	Identification and Characterization of Novel MicroRNAs from Schistosoma japonicum
作者	Xue, Xiangyang1,3; Sun, Jun 1; Zhang, Qingfeng 1; Wang, Zhangxun 1; Huang, Yufu 2; Pan, Weiqing 1,2
发表日期	2008-12-24
发表期刊	PLOS ONE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	RT-PCR ; RNA ; EXPRESSION ; GENE ; MANSONI ; QUANTIFICATION ; BIOGENESIS ; PATHWAYS
摘要	Background: Schistosomiasis japonica remains a major public health problem in China. Its pathogen, Schistosoma japonicum has a complex life cycle and a unique repertoire of genes expressed at different life cycle stages. Exploring schistosome gene regulation will yield the best prospects for new drug targets and vaccine candidates. MicroRNAs (miRNAs) are a highly conserved class of noncoding RNA that control many biological processes by sequence-specific inhibition of gene expression. Although a large number of miRNAs have been identified from plants to mammals, it remains no experimental proof whether schistosome exist miRNAs. Methodology and Results: We have identified novel miRNAs from Schistosoma japonicum by cloning and sequencing a small (18-26 nt) RNA cDNA library from the adult worms. Five novel miRNAs were identified from 227 cloned RNA sequences and verified by Northern blot. Alignments of the miRNAs with corresponding family members indicated that four of them belong to a metazoan miRNA family: let-7, miR-71, bantam and miR-125. The fifth potentially new (non conserved) miRNA appears to belong to a previously undescribed family in the genus Schistosome. The novel miRNAs were designated as sja-let-7, sja-miR-71, sja-bantam, sja-miR-125 and sja-miR-new1, respectively. Expression of sja-let-7, sja-miR-71 and sja-bantam were analyzed in six stages of the life cycle, i.e. egg, miracidium, sporocyst, cercaria, schistosomulum, and adult worm, by a modified stem-loop reverse transcribed polymerase chain reaction (RT-PCR) method developed in our laboratory. The expression patterns of these miRNAs were highly stage-specific. In particular, sja-miR-71 and sja-bantam expression reach their peaks in the cercaria stage and then drop quickly to the nadirs in the schistosomulum stage, following penetration of cercaria into a mammalian host. Conclusions: Authentic miRNAs were identified for the first time in S. japonicum, including a new schistosome family member. The different expression patterns of the novel miRNAs over the life stages of S. japonicum suggest that they may mediate important roles in Schistosome growth and development.
资助项目	National Basic Research Program (973 Program) in ChinaNational Basic Research Program of China [2007CB513100]
出版者	PUBLIC LIBRARY SCIENCE
出版地	SAN FRANCISCO
ISSN	1932-6203
卷号	3 期号:12
DOI	10.1371/journal.pone.0004034
页数	9
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000265463000025
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	19107204
SCOPUSEID	2-s2.0-58149202186
通讯作者地址	[Xue, Xiangyang]Institute for Infectious Diseases and Vaccine Development,Tongji University School of Medicine,China
Scopus学科分类	Multidisciplinary
引用统计	被引频次：90[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/6844
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学微生物学
通讯作者	Xue, Xiangyang
作者单位	1.Institute for Infectious Diseases and Vaccine Development,Tongji University School of Medicine,China; 2.Department of Pathogenic Biology,Second Military Medical University,China; 3.Department of Microbiology,Wenzhou Medical College,University-Town,China
第一作者单位	温州医科大学
推荐引用方式 GB/T 7714	Xue, Xiangyang,Sun, Jun,Zhang, Qingfeng,et al. Identification and Characterization of Novel MicroRNAs from Schistosoma japonicum[J]. PLOS ONE,2008,3(12).
APA	Xue, Xiangyang, Sun, Jun, Zhang, Qingfeng, Wang, Zhangxun, Huang, Yufu, & Pan, Weiqing. (2008). Identification and Characterization of Novel MicroRNAs from Schistosoma japonicum. PLOS ONE, 3(12).
MLA	Xue, Xiangyang,et al."Identification and Characterization of Novel MicroRNAs from Schistosoma japonicum".PLOS ONE 3.12(2008).