SD大鼠坐骨神经微血管三维可视化研究初探

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题名	SD大鼠坐骨神经微血管三维可视化研究初探
其他题名	A TRAIL OF THREE-DIMENSIONAL VISUALIZATION TECHNIQUE TO OBSERVE INTRANEURAL MICROVESSELS OF SCIATIC NERVES IN SPRAGUE DAWLEY RATS
作者	朱昭炜 1; 毛以华2; 何波 1; 周翔 1; 朱庆棠 1; 顾立强 1; 郑剑文 1; 朱家恺 1; 唐茂林2; 刘小林 1
发表日期	2012
发表期刊	中国修复重建外科杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	周围神经血管造影三维重建大鼠
摘要	目的探讨通过全身灌注伊凡斯蓝(Evan’s blue,EB)或氧化铅建立SD大鼠坐骨神经内微血管可视化模型的可行性,并比较其优劣。方法取15只健康成年SD大鼠,雌雄不限,200～250 g,随机分为传统显影组(A组)、荧光显影组(B组)和放射显影组(C组),每组5只,分别灌注明胶墨汁灌注剂、明胶EB灌注剂和明胶氧化铅灌注剂。灌注后4℃低温保存2 h取材,体视显微镜对各组进行观察后,反射荧光显微镜采集B组、micro-CT采集C组神经内微血管二维图像,导入计算机用Mimics 15.0软件进行三维重建。结果 3组均可在体视显微镜下观察到坐骨神经内微血管形态。反射荧光显微镜观察示,微血管管径为10～30μm。B、C组均可通过将获取的二维图像导入计算机清晰重现微血管三维结构。结论 EB、氧化铅灌注法均可对神经内微血管进行三维重建可视化观察,但EB存在易渗漏、图像噪点多、工作量大的缺点,不利于大样本研究;氧化铅虽分辨率较EB显影低,但易于操作,可用于大样本研究。
其他摘要	Objective To investigate the feasibil ity of establ ishing the visual ization models of intraneural microvessels of sciatic nerves in Sprague Dawley (SD) rats by systemic infusion of Evan’s blue (EB) or lead oxide and to compare the advantages and disadvantages. Methods Fifteen healthy adult SD rats of either gender, weighing 200-250 g, were randomly divided into traditional group (group A, n=5), fluorescence group (group B, n=5), and radiography group (group C, n=5). Ink, EB, and lead oxide, all mixed with gelatin solution, were injected in groups A, B, and C, respectively. After 2 hours of cryopreservation under 4°C, all sciatic nerves were harvested and observed through stereomicroscope to make sure the fill ing condition. The two-dimentional (2D) images were then collected via reflexion fluorescent microscope in group B and via micro-CT scan in group C. All images were imported into computer to establ ish three-dimentional (3D) reconstruction models by Mimics 15.0. Results All groups could show the outl ine of intraneural microvessels of sciatic nerves under stereomicroscope. Diameters of them were measured under fluorescent microscope, ranging from 10 μm to 30 μm. Both groups B and C could establ ish 3D reconstruction models from 2D images. These models could clearly reproduce the structure of microvessels. Conclusion Both EB and lead oxide can be used to establ ish 3D reconstruction models to observe structure of the intraneural vessels. However, EB has some disadvantages, such as predisposition to infiltration, grainy 2D images and time-consuming procedure; it is not suitable for researches of large specimen. Though 2D pictures from lead oxide have lower resolution than EB, it is easier to be manipulated and appropriate for experiments of large specimen.
资助项目	国家高技术研究发展计划（863）资助项目（2012AA020507）;中山大学先进技术研究院“面向产业发展的关键技术研究与应用”资助项目（90035-3283312）;广东省自然科学基金博士启动项目（S201204006336）;高等学校博士学科点专项科研基金（20120171120075）~~
ISSN	1002-1892
卷号	27 期号:02 页码:189-192
页数	4
收录类别	CNKI ; 北大核心 ; CSCD
学科领域	医药、卫生 ; 外科学
URL	查看原文
CSCD记录号	CSCD:4754243
CNKI分类号	R651.3
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/66603
专题	基础医学院（机能实验教学中心）
作者单位	1.中山大学附属第一医院显微创伤骨科; 2.温州医学院解剖教研室
推荐引用方式 GB/T 7714	朱昭炜,毛以华,何波,等. SD大鼠坐骨神经微血管三维可视化研究初探[J]. 中国修复重建外科杂志,2012,27(02):189-192.
APA	朱昭炜., 毛以华., 何波., 周翔., 朱庆棠., ... & 刘小林. (2012). SD大鼠坐骨神经微血管三维可视化研究初探. 中国修复重建外科杂志, 27(02), 189-192.
MLA	朱昭炜,et al."SD大鼠坐骨神经微血管三维可视化研究初探".中国修复重建外科杂志 27.02(2012):189-192.

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