姜黄素对小鼠肺缺血/再灌注损伤时细胞凋亡及CHOP的影响

doi:10.13459/j.cnki.cjap.2013.04.003

科研成果详情

题名	姜黄素对小鼠肺缺血/再灌注损伤时细胞凋亡及CHOP的影响
其他题名	Effects of curcumin on pneumocyte apoptosis and CHOP in pulmonary ischemia/ reperfusion injury of mice
作者	周俊辉 1,2; 郝卯林2; 赵珊 2; 陈海娥 2; 陈丹 2; 应磊2; 孙勤 3; 王万铁2
发表日期	2013-07-28
发表期刊	中国应用生理学杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	C/EBP同源蛋白肺姜黄素缺血/再灌注未折叠蛋白反应细胞凋亡小鼠
其他关键词	C/EBP homologous protein ; lung ; curcumin ; ischemia/reperfusion ; unfolded protein response ; apoptosis
摘要	目的:本研究旨在探讨姜黄素(CUR)对小鼠肺缺血/再灌注(I/R)损伤时细胞凋亡及CCAAT/增强子结合蛋白同源蛋白(CHOP)的影响。方法:C57BL/6J小鼠60只,随机分为6组(n=10):假手术组(Sham组)、缺血/再灌注组(I/R组)、缺血/再灌注+二甲基亚砜组(DMSO组)以及缺血/再灌注+姜黄素剂量100 mg/kg、150 mg/kg和200mg/kg组(CUR-100组、CUR-150组和CUR-200组)。分别于再灌注3 h留取左肺,检测湿干/重比(W/D)和总肺水含量(TLW);光镜和电镜观察肺组织形态学结构改变并进行肺组织损伤定量评估(IQA);逆转录-PCR、Western blot测定CHOP和葡萄糖调节蛋白78(GRP78)的mRNA和蛋白表达水平;原位末端标记法(TUNEL法)检测肺细胞凋亡指数(AI)。结果:与Sham组相比,I/R组和DMSO组中CHOP和GRP78mRNA和蛋白表达水平均升高(P<0.05),W/D、TLW、IQA和AI均明显增加(P<0.01),肺组织形态学和超微结构均发生明显损伤;与DMSO组相比,CUR-100组、CUR-150组和CUR-200组各组GRP78mRNA和蛋白表达水平均升高(P<0.05),而CHOPmRNA和蛋白表达水平均降低(P<0.05),W/D、TLW、IQA和AI亦均下降(P<0.01),肺组织形态学异常改变趋近于正常。结论:I/R引发小鼠肺组织细胞过度的未折叠蛋白反应,并通过CHOP途径引起细胞凋亡,损伤肺组织;姜黄素对I/R损伤发生的小鼠肺脏具有良好的保护作用,其机制可能与其对抗过度的未折叠蛋白反应中CHOP途径引起的细胞凋亡有关。
其他摘要	Objective: To investigate the effects of curcumin (CUR) on pneumocyte apoptosis and CCAAT/enhancer binding protein ho mologous protein (CHOP) in pulmonary ischemia/reperfusion injury (PIRI) in mice. Methods: Sixty C57BL/6J mice were randomly allocated into six groups ( n= 10) : Sham operation group (Sham group) , ischemia/ reperfusion group (I/R group) , ischemia/ reperfusion+ dimethyl sulfoxide group ( DMSO group) , ischemia/reperfusion + curcumin pre-treated with respectively 100 mg/kg, 150 mg/kg and 200 mg/ kg groups (CUR-100 group, CUR-150 group andCUR-200group). Left lung tissue of eachgroupwas excised after reperfusion for 3 h. Wet lung weight to dry lung weight (W/D) andtotallung water content (TLW) were tested. The morphological andultrastructural changesof lung tissue were observed under light microscope and electron microscope, and index of quantitative evaluation for alveolar damage (IQA) was calculated. The expression levels of CHOP and glucose regulated protein78 (GRP78) were detected by RT-PCR and Western Blot. Apoptosis index (AI) of lung tissuewas determined by terminal deoxynuleotidy1transferase mediated dUTP nick end labeling (TUNEL) method. Results: Compared with Sham group, the expression levels of CHOP, GRP78 mRNA and protein were all significantly increased ( P< 0. 05) in I/R group and DMSO group, W/D, TLW, IQA andAI were all notably higher ( P< 0. 01) ; morphological and ultrastructural injury in lung tissue were no tably observed in I/R group. Compared with DMSO group, the expression levels of GRP78 mRNA and protein were increased higher ( P< 0. 05) in CUR-100group, CUR-150 group, and CUR-200 group, but the expression levels of CHOP mRNA and protein were decreased lower ( P< 0. 05) , W/ D, TLW, IQA andAI were also decreased (P< 0. 05, P< 0. 01) ; morphological and ultrastructural injury in lung tissue were gradually alleviated in CUR groups. Conclusion: I/R induces excessive unfoldedprotein response (UPR) in lung tissue, inwhich CHOP par ticipates in pneumocyte apoptosis, leading to lung injury; CUR has notable effects on lung protection against I/R injury, which may be related to inhibition of apoptosis mediated by CHOP in excessive UPR.
资助项目	浙江省公益技术应用研究项目（2011C37092）;浙江省医药卫生科技计划项目（2010KYA132）;浙江省中医药科技计划项目（2010ZB088）
ISSN	1000-6834
卷号	29 期号:04 页码:318-323+386
DOI	10.13459/j.cnki.cjap.2013.04.003
页数	7
收录类别	CNKI ; 万方 ; CSCD ; PKU ; 北大核心 ; ISTIC
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:4903943
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/64982
专题	附属第一医院基础医学院（机能实验教学中心）_机能学系_生理学其他_附属慈溪医院（慈溪市人民医院）
作者单位	1.温州医学院附属第一医院麻醉科; 2.温州医学院病理生理学系缺血/再灌注研究室; 3.温州医学院附属慈溪医院ICU
第一作者单位	附属第一医院; 麻醉科; 温州医科大学
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	周俊辉,郝卯林,赵珊,等. 姜黄素对小鼠肺缺血/再灌注损伤时细胞凋亡及CHOP的影响[J]. 中国应用生理学杂志,2013,29(04):318-323+386.
APA	周俊辉., 郝卯林., 赵珊., 陈海娥., 陈丹., ... & 王万铁. (2013). 姜黄素对小鼠肺缺血/再灌注损伤时细胞凋亡及CHOP的影响. 中国应用生理学杂志, 29(04), 318-323+386.
MLA	周俊辉,et al."姜黄素对小鼠肺缺血/再灌注损伤时细胞凋亡及CHOP的影响".中国应用生理学杂志 29.04(2013):318-323+386.

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