回肠间置术治疗大鼠非肥胖性2型糖尿病的疗效

doi:10.3760/cma.j.issn.1007-631X.2013.08.016

科研成果详情

题名	回肠间置术治疗大鼠非肥胖性2型糖尿病的疗效
其他题名	Effects of ileal transposition on non-obese type 2 diabetic rats
作者	郑志海; 朱恒梁; 阮小蛟; 郑晓风
发表日期	2013-08-25
发表期刊	中华普通外科杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	糖尿病 2型吻合术外科胰高血糖素样肽1 疾病模型动物
其他关键词	Diabetes mellitus,type 2 ; Anastomosis,surgical ; Glucagon-like peptide-1 ; Disease models,animal
摘要	目的探讨回肠间置术治疗自发性非肥胖性2型糖尿病GK大鼠的疗效及其可能的机制。方法将20只GK大鼠随机分为回肠间置术组和假手术组,每组10只。分别测定术前(0周)、术后1、4、8、16、24周各组大鼠体重、日均摄食量和空腹血糖水平;术前(0周)、术后4、8、16、24周各组大鼠葡萄糖耐量试验(OGTT)和血清胰高血糖素样肽1(glucagon-like peptide-1,GLP-1)浓度;术前(0周)、术后4、24周空腹胰岛素浓度,并计算胰岛素抵抗指数。结果与术前相比,术后1周两组大鼠体重和摄食量均显著下降(P<0.05),之后两组大鼠体重和摄食量均逐渐增加,但在术后1~24周两组大鼠体重和摄食量相比差异均无统计学意义(均P>0.05)。术后1周,两组大鼠空腹血糖[回肠间置术组(6.1±0.6)mmol/L,假手术组(6.2±0.8)mmol/L]均低于术前[分别为(7.0±0.5)mmol/L和(6.9±0.5)mmol/L],P<0.05。之后两组大鼠空腹血糖均有所回升,术后8~24周回肠间置术组大鼠空腹血糖缓慢下降,均显著低于假手术组(均P<0.05)。术后4~24周,回肠间置术组OGTT与假手术组相比有明显改善(P<0.05)。在术后24周,回肠间置术组空腹胰岛素水平较假手术组低[(0.26±0.08)ng/ml比(0.42±0.09)ng/ml],P<0.05,但回肠间置术组胰岛素抵抗指数明显下降[(1.1±0.4比2.6±0.4),P<0.05]。术后4~24周,回肠间置术组葡萄糖负荷后30min GLP-1浓度明显升高(P<0.01),而假手术组则未见显著变化(P>0.05)。结论回肠间置术治疗非肥胖性T2DM大鼠是有效的,术后GLP-1升高可能是糖代谢改善的机制。
其他摘要	Objective To evaluate effects and possible mechanisms of ileal transposition on spontaneous non-obese type 2 diabetic GK rats. Methods 20 GK rats were randomly divided into two groups: ileal transposition group and sham operation group (n= 10). We observed and determined the weight change, daily average food consumption and FBG (fasting blood-glucose) level of rats before the operation(0 weeks) and 1,4,8,16 and 24 weeks after the surgery. Glucose tolerance test (GTT) was carried out and GLP-1 (glucagon-like peptide-1) concentration measured before the operation(0 weeks) and 4,8,16 and 24 weeks after the surgery and the fasting insulin concentration before the surgery(0 weeks) and 4,24 weeks after the surgery measured, and the indicator of HOMA-IR calculated. Results There was no significant difference in the operating time between the two groups [(87±8) min vs. (84±7) min],P>0.05. Compared with those before surgery, body weight and food consumption of the two groups of rats decreased significantly a week after surgery (P< 0. 05),and then the body weight and food consumption of the two groups of rats all gradually increased, but the difference of the two groups of rats has no statistical significance 1-24 weeks after surgery (P >0.05). The FBG of the two groups of rats a week after surgery [ileal transposition group (6.1±0.6)mmol/L, sham operation group (6.2±0.8)mmol/L] decreased significantly compared with that before surgery[ (7.0 ±0.5) mmol/L and (6.9 ±0.5) mmol/L] (P< 0.05), and then FBG of the two groups of rats all rose again. The FBG of the rats in surgery group decreased slowly from 8 to 24 weeks after surgery, while the FBG of the rats in the sham surgery group maintained the preoperative level, and the differences of the FBG of the two groups all have statistical significance 8-24 weeks after surgery (P < 0. 05). Four weeks after surgery, OGTT of the ileal transposition group significantly improved (P<0. 01). 24 weeks after surgery, fasting insulin levels of the ileal transposition group were lower [ (0. 26 ± 0. 08) ng/mL vs. (0. 42 ± 0. 09) ng/ml ], P < 0. 05. Compared with the sham surgery group, and HOMA-IR was lower (1.1 ±0. 4) vs. (2. 6 ±0.4), P <0. 05. Four weeks after surgery, oral glucose-stimulated peak (30 min) levels of blood GLP-1 increased markedly in operation groups after surgery (P <0. 01). Conclusions Ileal transposition is effective for the treatment of non-obese T2DM rats, and the control of blood glucose does not depend on the reduction of body weight and food comsumption, and the high secretion of GLP-1 after ileal interposition seem to be helpful in diabetes control.
资助项目	温州市科委（国际合作课题）资助项目（H20090034）
ISSN	1007-631X
卷号	28 期号:08 页码:615-619
DOI	10.3760/cma.j.issn.1007-631X.2013.08.016
页数	0
收录类别	CNKI ; 万方 ; 北大核心 ; ISTIC ; CSCD ; PKU
学科领域	医药、卫生 ; 外科学
URL	查看原文
CSCD记录号	CSCD:4908472
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/64856
专题	附属第一医院_腔镜外科
作者单位	温州医学院附属第一医院腔镜外科
第一作者单位	附属第一医院_腔镜外科
第一作者的第一单位	附属第一医院_腔镜外科
推荐引用方式 GB/T 7714	郑志海,朱恒梁,阮小蛟,等. 回肠间置术治疗大鼠非肥胖性2型糖尿病的疗效[J]. 中华普通外科杂志,2013,28(08):615-619.
APA	郑志海, 朱恒梁, 阮小蛟, & 郑晓风. (2013). 回肠间置术治疗大鼠非肥胖性2型糖尿病的疗效. 中华普通外科杂志, 28(08), 615-619.
MLA	郑志海,et al."回肠间置术治疗大鼠非肥胖性2型糖尿病的疗效".中华普通外科杂志 28.08(2013):615-619.