抗肿瘤坏死因子单抗对脂多糖诱导的人牙周膜成纤维细胞TRAIL和TNF-α的影响

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题名	抗肿瘤坏死因子单抗对脂多糖诱导的人牙周膜成纤维细胞TRAIL和TNF-α的影响
其他题名	Effect of anti-tumor necrosis factor antibody on TRAIL and TNF-α expression induced by lipopolysaccharide in human periodontal ligament fibroblasts
作者	陈焱 1; 叶斌 2; 莫文元 3; 俞诚波 1; 林晡 1; 尹向鹏 1; 许红苗 1
发表日期	2015
发表期刊	中国临床药理学与治疗学影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	人牙周膜成纤维细胞肿瘤坏死因子 TRAIL 脂多糖抗肿瘤坏死因子单抗
其他关键词	human periodontal ligament fibroblasts ; tumor necrosis factor ; TNF-related apoptosis-inducing ligand ; lipopolysaccharide ; anti-tumor necrosis factor antibody ; periodontal disease
摘要	目的:观察脂多糖对人牙周膜成纤维细胞(HPLFs)肿瘤坏死因子(TNF)受体相关凋亡诱导配体(TRAIL)和TNF-α的诱导作用及抗肿瘤坏死因子单抗(抗TNF单抗)对其的影响,探讨TNF超家族参与牙周病的可能作用机制。方法:HPLFs培养至第6代,加入不同浓度的脂多糖(0、0.1、1、10、100μg/m L)培养24 h,分为命名为Z0组、Z0.1组、Z1组、Z10组、Z100组,并取Z1组浓度的脂多糖,在加药的同时加抗TNF单抗75μg/m L(Z1+75组)。分别采用实时荧光定量PCR法或Western blot法测定TRAIL和TNF-αm RNA或蛋白的表达。结果:Z1组、Z10组HPLFs TRAIL m RNA的表达水平显著高于Z0组或Z0.1组(均P<0.05),Z1组与Z10组之间差异无统计学意义(P>0.05);Z100组显著高于Z0组(P<0.05)且与Z0.1组、Z1组和Z10组比较差异无统计学意义(均P>0.05)。Z1组或Z10组TNF-αm RNA的表达水平显著高于Z0组或Z100组(均P<0.05);Z1组与Z10组之间,Z0组、Z0.1组和Z100组之间差异无统计学意义(均P>0.05)。HPLFs TRAIL蛋白的表达水平在Z100组0.05)。抗TNF单抗治疗后TRAIL m RNA及蛋白和TNF-αm RNA的表达水平显著低于Z1组(P<0.05和P<0.01)。TRAIL和TNF-αm RNA的表达水平呈显著直线正相关(r=0.819,n=30,P<0.01)。结论:HPLFs经脂多糖诱导后,其TRAIL和TNF-α的表达呈现先升高后下降,且随脂多糖的浓度变化而相应地变化,这种诱导作用能被抗TNF单抗所抑制。
其他摘要	AIM: To investigate the possible roles of tumor necrosis factor(TNF) superfamily in the pathogenesis of periodontal disease inflammation, the effect of anti-tumor necrosis factor antibody on TRAIL and TNF-α expression induced by lipopolysaccharide in human periodontal ligament fibroblasts is observed. METHODS: HPLFs were cultured to the sixth generation cells and were interfered with defferent concentrations of lipopolysaccharide(0,0.1,1,10,100 μg /mL,respectively) for 24 h, named group Z_0,Z_(0.1),Z_1,Z_(10) and Z_(100). HPLFs were also cultured with 1 μg /mL lipopolysaccharide and 75 μg /mL anti-TNF antibody (named group Z_1 + 75). The expressions of both TNF-α and TRAIL mRNA were analysed by Real-time quantitative reverse transcription polymerase chain reaction(QRTPCR), while TRAIL protein were determined by West Blot methods. RESULTS: The expressions of TRAIL mRNA in group Z_1 or Z_(10) were significantly higher than those in group Z_0 or Z_(0.1) (all P < 0.05), in group Z_(100) were significantly higher than those in group Z_0 (P < 0.05). But there were no significant differences neither between group Z_1 and Z_(10) nor among group Z_(100),Z_(0.1),Z_1 and Z_(10)(all P > 0.05). Moreover,the expressions of TNF-α mRNA in group Z_1 or Z_(10) were significantly higher than those in group Z_0 or Z_(100) (all P < 0.05),whereas there were nonsignificant differences neither between group Z_1 and Z_(10) nor among group Z_0,Z_(0.1),and Z_(100) (all P >0.05). Dramatically,the expressions of TRAIL protein were significant difference between each groups, in group Z_(100) < Z_0 < Z_(0.1) < Z_1 (all P < 0.05 or P <0.01). And it was significantly higher in group Z_(10) than those in group Z_0 or Z_(100) (all P < 0.01),while there were nonsignificant differences neither between group Z_(10) and Z_(0.1) nor among group Z_(10) and Z_1 (all P > 0.05). Noteworthy, the expressions of either TRAIL mRNA and protein or TNF-α mRNA in group Z_1 + 75 were significantly decreased than those in group Z_1 (P < 0.05 or < 0.01,respectively). Furthermore, there were significance positive correlation between levels of TRAIL and TNF-α mRNA (r =0.819,n = 30,P < 0.01). CONCLUSION: The results shows that the expressions of TRAIL and TNF-α in HPLFs can be induced by lipopolysaccharide, the levels of them were elevated in the first and decreased subsequently,this changes were accompanied by lipopolysaccharide concentration. Date also show the function of lipopolysaccharide can be inhibited by anti-TNF antibody.
资助项目	温岭市科技局基金资助项目（2013-2-111）
ISSN	1009-2501
卷号	20 期号:05 页码:493-498
页数	6
收录类别	CNKI ; 万方 ; CSCD ; ISTIC
学科领域	医药、卫生 ; 口腔科学
URL	查看原文
CSCD记录号	CSCD:5448017
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63983
专题	附属第一医院_口腔科
作者单位	1.温州医学院附属温岭市医院口腔科; 2.台州市中心医院儿科; 3.台州市中西医结合医院外科
第一作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	陈焱,叶斌,莫文元,等. 抗肿瘤坏死因子单抗对脂多糖诱导的人牙周膜成纤维细胞TRAIL和TNF-α的影响[J]. 中国临床药理学与治疗学,2015,20(05):493-498.
APA	陈焱., 叶斌., 莫文元., 俞诚波., 林晡., ... & 许红苗. (2015). 抗肿瘤坏死因子单抗对脂多糖诱导的人牙周膜成纤维细胞TRAIL和TNF-α的影响. 中国临床药理学与治疗学, 20(05), 493-498.
MLA	陈焱,et al."抗肿瘤坏死因子单抗对脂多糖诱导的人牙周膜成纤维细胞TRAIL和TNF-α的影响".中国临床药理学与治疗学 20.05(2015):493-498.

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