Effects of qingxin kaiqiao recipe volatile oil on expressions of GFAP and caspase-3 in the cortex and hippocampus of AD rats

科研成果详情

题名	Effects of qingxin kaiqiao recipe volatile oil on expressions of GFAP and caspase-3 in the cortex and hippocampus of AD rats
作者	Hai-Yan Hu; Lei Lei; Xiang Chen; Wen-Hua Wang; Xiao-Yan Zhang
发表日期	2013-07-01
发表期刊	Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine 影响因子和分区
语种	英语
原始文献类型	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
摘要	Objective: To study effects of Qingxin Kaiqiao Recipe (QKR) and its volatile oil on the expressions of Abeta(25-35) glial fibrillary acidic protein (GFAP), beta-amyloid (Abeta), beta-amyloid precursor protein (betaAPP), and Caspase-3 in the cortex and hippocampus of Alzheimer's disease (AD) rats induced by injecting Abeta(25-35) into the bilateral amygdala. Methods: Totally 32 male SD rats were selected. The AD rat model was establish by injecting Abeta(25-35) from bilateral amygdala. After modeling they were randomly divided into the model group, the Donepezil Hydrochloride group [Donepezil Hydrochloride Tablet (1.67 mg/kg), abbreviated as the DH group], the QKR group (QKR Decoction, 12.67 mL/kg), and the volatile oil group (3.33 mL/kg), 8 rats in each group. Another 8 rats were selected as the normal control group. Equal volume of double distilled water was administered to rats in the normal control group and the model group by gastrogavage, once daily for 2 successive weeks. The Morris water maze test was performed by the end of medication. The escape latency and times of crossing the platform in the water maze test were recorded during the 1st day to the fifth day. The expressions of GFAP, Abeta, betaAPP, and Caspase-3 in the cortex and hippocampus of the rats in each group were detected by immunohistochemical assay. Results: Compared with the model group, the escape latency from the 3rd day to the 5th day was shortened, the expressions of GFAP, Abeta, betaAPP, and Caspase-3 decreased in the cortex and hippocampus, the times of crossing the platform increased in each medication group, showing statistical difference (P < 0.01, P < 0.05). Compared with the DH group, the expressions of Abeta in the cortex and hippocampus decreased, and the betaAPP expression increased in the QKR group. The expressions of GFAP, betaAPP, and Caspase-3 in the cortex and hippocampus increased in the volatile oil group. The escape latency from the 3rd day to the 5th day was obviously prolonged, and the times of crossing the platform decreased in the volatile oil group, showing statistical difference (P < 0.05, P < 0.01). Conclusion: QKR could obviously improve the learning and memory capabilities of AD rats, which might be achieved through decreasing the expressions of GFAP, Abeta, betaAPP, and Caspase-3 in the cortex and hippocampus.
ISSN	1003-5370
卷号	33 期号:7 页码:927-32.
收录类别	PUBMED
URL	查看原文
PubMed ID	24063215
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63687
专题	第二临床医学院、附属第二医院、育英儿童医院_中医系
作者单位	Department of Traditional Chinese Medicine, Second Clinical College of Wenzhou Medical College, Zhejiang 325003, China. wyzhongyixi@163.com
第一作者单位	中医系
第一作者的第一单位	中医系
推荐引用方式 GB/T 7714	Hai-Yan Hu,Lei Lei,Xiang Chen,et al. Effects of qingxin kaiqiao recipe volatile oil on expressions of GFAP and caspase-3 in the cortex and hippocampus of AD rats[J]. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine,2013,33(7):927-32..
APA	Hai-Yan Hu, Lei Lei, Xiang Chen, Wen-Hua Wang, & Xiao-Yan Zhang. (2013). Effects of qingxin kaiqiao recipe volatile oil on expressions of GFAP and caspase-3 in the cortex and hippocampus of AD rats. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine, 33(7), 927-32..
MLA	Hai-Yan Hu,et al."Effects of qingxin kaiqiao recipe volatile oil on expressions of GFAP and caspase-3 in the cortex and hippocampus of AD rats".Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine 33.7(2013):927-32..