Detection of WU polyomavirus in children with low respiratory tract infections using real-time fluorescent quantitative PCR

科研成果详情

题名	Detection of WU polyomavirus in children with low respiratory tract infections using real-time fluorescent quantitative PCR
作者	Hong-Xia Lin; Chang-Hua Zgeng; Zhi-Hui Zheng; Hou-Xian Ouyang; Min-Qiao Zheng; Feng Wu; Feng Lin; Jian-Yi Hou; Jian-Xin Lv
发表日期	2012-04-01
发表期刊	Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: Development and application of a real time fluorescent quantitative PCR (FQ-PCR) assay for detecting WU polyomavirus in children with low respiratory tract infections. Methods: The VP2 gene of WU polyomavirus was selected as the detection target, from which the real time primers and probes were designed. The standard curve was established by using recombinant plasmid as template. And the FQ-PCR assay for specific detection of WU polyomavirus was established. The specificity, sensitivity and reproducibility of the method were evaluated. Furthermore, the clinical specimens from children with respiratory tract infections collected in Wenling First People's Hospital were quantitatively detected using this method. Results: In this study, the FQ-PCR method was established to detect a specific fragment in VP2gene of WU polyomavirus. The standard curve coefficient R2 was 0.998. And this method can detect as low as 50 copies recombinant plasmid. The clinical specimens of sputum and throat swab from children with respiratory tract infections were quantitatively detected using this method. 7 sputum specimens were detected as WU polyomavirus positive in 700 sputum specimens, the positive ratio was 1.00%. No positive specimens were detected in 146 specimens of throat swabs and 846 blood samples from same patient population. Conclusion: The results indicated that the FQ-PCR assay method established in this study was specific, rapid and sensitive for detecting WU polyomavirus in children with lower respiratory tract infections. The sputum specimen is more suitable to be used for gene detection of WU polyomavirus than throat swab or blood.
ISSN	1003-9279
卷号	26 期号:2 页码:150-2.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	23002562
Scopus记录号	2-s2.0-84867614899
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63529
专题	其他_附属温岭医院（温岭市第一人民医院）
作者单位	Wenzhou Medical College, Wenling Affiliated Hospital of Wenzhou Medical College, Zhejiang 317500, China.
第一作者单位	附属温岭医院（温岭市第一人民医院）
第一作者的第一单位	附属温岭医院（温岭市第一人民医院）
推荐引用方式 GB/T 7714	Hong-Xia Lin,Chang-Hua Zgeng,Zhi-Hui Zheng,et al. Detection of WU polyomavirus in children with low respiratory tract infections using real-time fluorescent quantitative PCR[J]. Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology,2012,26(2):150-2..
APA	Hong-Xia Lin., Chang-Hua Zgeng., Zhi-Hui Zheng., Hou-Xian Ouyang., Min-Qiao Zheng., ... & Jian-Xin Lv. (2012). Detection of WU polyomavirus in children with low respiratory tract infections using real-time fluorescent quantitative PCR. Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, 26(2), 150-2..
MLA	Hong-Xia Lin,et al."Detection of WU polyomavirus in children with low respiratory tract infections using real-time fluorescent quantitative PCR".Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology 26.2(2012):150-2..