Effects of tensile strain on the expression of Runx2/Cbfalpha1 in osteoblasts cultivated with inflammatory stimulus

科研成果详情

题名	Effects of tensile strain on the expression of Runx2/Cbfalpha1 in osteoblasts cultivated with inflammatory stimulus
作者	Rong-dang Hu; Jie Ye; Chun-yan Xu
发表日期	2011-11-01
发表期刊	Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 影响因子和分区
语种	英语
原始文献类型	Journal Article ; Research Support, Non-U.S. Gov't
摘要	Objective: To determine the effects of tensile strain on the expression of core binding factor alpha1 (Runx2/Cbfalpha1) in osteoblasts cultivated in Pg-LPS stimulated RAW264.7 culture supernatant. Methods: Osteoblasts MC3T3-E1 were cultivated in LPS stimulated monocyte culture supernatant for 24 h. Then, a four-point bending device was used to perform a single period of tensile strain (1000 microm strain, 3000 microm strain respectively) on osteoblasts for 0 h, 0.5 h, 1 h, 2 h, 3 h, 6 h. Non-inflammatory stimuli were set up as control. The expression of Runxr2/Cbfalpha1 mRNA in MC3T3-E1 cells was examined by real-time RT-PCR. Results: With the mechanical loading, the expression of Runx2/Cbfalpha1 mRNA increased in both inflammatory and non-inflammatory groups of osteoblasts and reached the peak after 6 h. The inflammatory group had lower expression of Runx2/Cbfalpha1 mRNA than that of the non-inflammatory group (P<0.05). The varied strain level did not change the expression of Runx2/Cbfalpha1 mRNA within 3 hours. But after 6 hours, the 3000 microm strain induced higher expression of Runx2/Cbfalpha1 mRNA than the 1000 microm strain. Conclusion: Inflammation inhibits the tensile strain induced expression of Runx2/Cbfalpha1 in osteoblasts. Precaution needs to be paid to orthodontic treatment on patients with quiescent periodontitis.
ISSN	1672-173X
卷号	42 期号:6 页码:827-30.
收录类别	PUBMED
URL	查看原文
PubMed ID	22332552
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63499
专题	口腔医学院、附属口腔医院_正畸科
作者单位	Department of Orthodontics, the Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, China.
第一作者单位	口腔医学院、附属口腔医院_正畸科
第一作者的第一单位	口腔医学院、附属口腔医院_正畸科
推荐引用方式 GB/T 7714	Rong-dang Hu,Jie Ye,Chun-yan Xu. Effects of tensile strain on the expression of Runx2/Cbfalpha1 in osteoblasts cultivated with inflammatory stimulus[J]. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition,2011,42(6):827-30..
APA	Rong-dang Hu, Jie Ye, & Chun-yan Xu. (2011). Effects of tensile strain on the expression of Runx2/Cbfalpha1 in osteoblasts cultivated with inflammatory stimulus. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 42(6), 827-30..
MLA	Rong-dang Hu,et al."Effects of tensile strain on the expression of Runx2/Cbfalpha1 in osteoblasts cultivated with inflammatory stimulus".Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 42.6(2011):827-30..