Effect of hydroquinone on expression of topoisomerase enzyme IIα in human bone marrow mononuclear cells

科研成果详情

题名	Effect of hydroquinone on expression of topoisomerase enzyme IIα in human bone marrow mononuclear cells
作者	Yi-fen Shi; Kang Yu; Yi Chen; Xing-zhou Ren; Lai-xi Bi; Hong-lan Qian
发表日期	2010-09-01
发表期刊	Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: To investigate the effects of hydroquinone (HQ) on expression of topoisomerase IIα (TOPOIIα) in human bone marrow mononuclear cells, and to explore the role and possible regulatory mechanism of TOPOIIα involved in toxicity of HQ to hematopoietic cells. Methods: After human bone marrow mononuclear cells were exposed to 50 µmol/L HQ (used the cells which were exposed to sterile distilled water as control); the activity of TOPOII was measured by TOPOII assay kit; the expression levels of TOPOIIα mRNA and protein were detected by RT-PCR technique and Western blotting method respectively; the chromatin immunoprecipitation (ChIP) assay was carried out to study the possible mechanism of TOPOIIα expression changes. Results: (1) The activity of TOPOII was inhibited obviously; the protein and mRNA expression of TOPOIIα were 0.017 ± 0.029 and 0.610 ± 0.128, significantly lower than that in the control with the significant difference (P < 0.01) after treated with HQ for 10 h; (2) The decreased content of TOPOIIα was associated with descended level of histone H4 acetylation than in the control, from 1.198 ± 0.056 to 0.324 ± 0.229, with the significant difference (P < 0.01), without accompanied descended level of histone H3 acetylation, from 1.253 ± 0.045 to 1.177 ± 0.025 (P > 0.05); (3) TOPOIIα mRNA expression decreased gradually after HQ processing, and the chemical modification (histone H4 acetylation) of TOPOIIα promoter happened prior to the mRNA expression. Conclusion: HQ could repress the expression of TOPOIIα in human bone marrow mononuclear cells; the change of histone chemical modification plays an important role in the benzene's hematopoietic toxicity.
ISSN	1001-9391
卷号	28 期号:9 页码:660-3.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	21126478
Scopus记录号	2-s2.0-84865841845
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63389
专题	附属第一医院_血液内科
作者单位	Department of Hematology, Afficiated One of Wenzhou Medical College, Wenzhou 325000, China.
第一作者单位	血液内科; 血液内科
第一作者的第一单位	血液内科
推荐引用方式 GB/T 7714	Yi-fen Shi,Kang Yu,Yi Chen,et al. Effect of hydroquinone on expression of topoisomerase enzyme IIα in human bone marrow mononuclear cells[J]. Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases,2010,28(9):660-3..
APA	Yi-fen Shi, Kang Yu, Yi Chen, Xing-zhou Ren, Lai-xi Bi, & Hong-lan Qian. (2010). Effect of hydroquinone on expression of topoisomerase enzyme IIα in human bone marrow mononuclear cells. Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases, 28(9), 660-3..
MLA	Yi-fen Shi,et al."Effect of hydroquinone on expression of topoisomerase enzyme IIα in human bone marrow mononuclear cells".Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 28.9(2010):660-3..