Expression of Rock2 and TGF-β1 mRNA and glucocorticoid intervention in asthmatic rats

科研成果详情

题名	Expression of Rock2 and TGF-β1 mRNA and glucocorticoid intervention in asthmatic rats
作者	Chang-Hua Zheng; Xia-Sheng Tong; Bin-Fei Li; Wei-Xi Zhang; Chang-Chong Li
发表日期	2010-11-01
发表期刊	Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Asthma Glucocorticoid Rats Rhoassociated coiled coil forming protein kinase Transforming growth factor
摘要	Objective: To study the roles of rhoassociated coiled coil forming protein kinase 2 (Rock2) and transforming growth factor-β1 (TGF-β1) mRNA in acute asthma and the effect of glucocorticoid intervention on the Rock2 and TGF-β1 mRNA expression in rats. Methods: Forty-eight male rats were randomly divided into 4 groups (n=12 each): asthma, control, dexamethasone treated (DXM) and budesonide treated (BUD). Rat model of asthma was prepared by the ovalbumin (OVA) challenge. The animals were sacrificed 24 hrs after the last challenge. The total cell number and differentiation cell number were counted in bronchoalveolar lavage fluid (BALF). The protein expression of Rock2 was ascertained by immunohistochemistry and the mRNA expression of TGF-β1 was ascertained by hybridization in situ. Results: The pathological changes in the BUD and the DXM groups were alleviated when compared with the asthma group. The total cell number and the percentage of eosinophil (EOS), polymorphonuclear leukocytes (PMN) and lymphocytes (Lym) in BALF in the asthma group were significantly higher than those in the control group (P<0.01). The percentage of macrophage (Mф) in the asthma group was significantly lower than that in the control group (P<0.01). The total cell number and the percentage of EOS and Lym in BALF in the DXM and the BUD groups decreased, while the percentage of Mф increased significantly compared with those in the asthma group (P<0.01). The Rock2 and TGF-β1 mRNA expression in lung tissues in the asthma group increased significantly compared with those in the control, BUD and DXM groups, while there were no significant differences in the Rock2 expression and TGF-β1 mRNA expression between the DXM or BUD group and the control group. Conclusions: The expression of Rock2 and TGF-β1 mRNA in lung tissues is increased in rats with acute asthma. Glucocorticoids can significantly decrease the expression of Rock2 and TGF-β1 in lung tissues, thus alleviates airway inflammation.
ISSN	1008-8830
卷号	12 期号:11 页码:877-81.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	21083983
Scopus记录号	2-s2.0-78650079810
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63374
专题	其他_附属温岭医院（温岭市第一人民医院）
作者单位	Wenling Affiliated Hospital, Wenzhou Medical College, Wenzhou, Zhejiang 317500, China. zhengchanghua0402@163.com
第一作者单位	附属温岭医院（温岭市第一人民医院）
第一作者的第一单位	附属温岭医院（温岭市第一人民医院）
推荐引用方式 GB/T 7714	Chang-Hua Zheng,Xia-Sheng Tong,Bin-Fei Li,et al. Expression of Rock2 and TGF-β1 mRNA and glucocorticoid intervention in asthmatic rats[J]. Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics,2010,12(11):877-81..
APA	Chang-Hua Zheng, Xia-Sheng Tong, Bin-Fei Li, Wei-Xi Zhang, & Chang-Chong Li. (2010). Expression of Rock2 and TGF-β1 mRNA and glucocorticoid intervention in asthmatic rats. Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, 12(11), 877-81..
MLA	Chang-Hua Zheng,et al."Expression of Rock2 and TGF-β1 mRNA and glucocorticoid intervention in asthmatic rats".Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics 12.11(2010):877-81..

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