Immunogenicity of multi-epitopes gene of major outer membrane protein of Chlamydia trachomatis

科研成果详情

题名	Immunogenicity of multi-epitopes gene of major outer membrane protein of Chlamydia trachomatis
作者	Shan-li Zhu; Zhao-hui Shi; Wen-shu Li; Jun Chen; Li-fang Zhang
发表日期	2009-03-01
发表期刊	Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: To construct a recombinant eukaryotic expression plasmid pcDNA3.1-Ct MOMP168 including Ct MOMP multi-epitopes gene, and evaluate the Ct MOMP-specific humoral and cellular immune response induced by pcDNA3.1-Ct MOMP168 in BALB/c mice. Methods: Recombinant plasmid pcDNA3.1-Ct MOMP168 including Ct MOMP multi-epitopes gene was constructed. Then, BALB/c mice were randomly assigned to receive (intramuscular injection) either pcDNA3.1-Ct MOMP168 or pcDNA3.1 or PBS (n = 12, 100 microg/time per mouse), and the same immunization schedule was repeated for the third time at 2 week intervals. The titers of anti-Ct MOMP antibody and its antibody subtypes in sera, the cytotoxicity of Ct MOMP-specific cytotoxic T lymphocyte (CTL) in spleen, and the level of cytokine (IFN-gamma, IL-4, IL-10)-producing CD3(+) T cells in spleen were detected by ELISA, LDH release assays and intracellular cytokine staining-fluorescence activated cell sorter (ICS-FACS), respectively. Results: The recombinant plasmid pcDNA3.1-Ct MOMP168 was able to induce Ct-specific antibody response (A(490) = 0.973 +/- 0.136; serum titer was 1:1000) as compared with pcDNA3.1 (A(490) = 0.180 +/- 0.025) and PBS (A(490) = 0.110 +/- 0.015), and the major antibody subtype was IgG2a with statistical significance (F = 106.884, P < 0.05). When the ratio of effector cells and target cells reached to 50:1, the activity of cytotoxic T-lymphocyte in pcDNA3.1-Ct MOMP168 immunized mice (41.71% +/- 8.34%) was significantly higher (F = 22.315, P < 0.05) than that in pcDNA3.1 immunized mice (18.40% +/- 3.45%) and PBS immunized mice (14.50% +/- 2.42%). The levels of CD3(+) IFN-gamma(+) T cells in pcDNA3.1-Ct MOMP168 immunized mice (1.15% +/- 0.16%) were significantly higher (F = 99.638, P < 0.05) than that in pcDNA3.1 immunized mice (0.12% +/- 0.08%) and PBS immunized mice (0.09% +/- 0.03%), while the significant difference in the levels of IL-4(+) CD3(+) T cells and IL-10(+) CD3(+) T cells was not observed (F = 0.886 and 1.112, P > 0.05) between pcDNA3.1-Ct MOMP168 immunized mice (0.13% +/- 0.08% and 0.14% +/- 0.08%) and pcDNA3.1 (0.07% +/- 0.05% and 0.13% +/- 0.06%) or PBS immunized mice (0.08% +/- 0.04% and 0.07% +/- 0.04%). Conclusion: In BALB/c mice, the recombinant plasmid pcDNA3.1-Ct MOMP168 might induce not only the generation of Ct-specific antibody, but also the high level of Ct MOMP-specific CD3(+) IFN-gamma(+) T cells.
ISSN	0253-9624
卷号	43 期号:3 页码:232-6.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	19534931
Scopus记录号	2-s2.0-70149097135
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63264
专题	检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系
作者单位	Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Wenzhou Medical College, Wenzhou, China.
第一作者单位	病原生物学与免疫学系; 病原生物学与免疫学系
第一作者的第一单位	病原生物学与免疫学系
推荐引用方式 GB/T 7714	Shan-li Zhu,Zhao-hui Shi,Wen-shu Li,et al. Immunogenicity of multi-epitopes gene of major outer membrane protein of Chlamydia trachomatis[J]. Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine],2009,43(3):232-6..
APA	Shan-li Zhu, Zhao-hui Shi, Wen-shu Li, Jun Chen, & Li-fang Zhang. (2009). Immunogenicity of multi-epitopes gene of major outer membrane protein of Chlamydia trachomatis. Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine], 43(3), 232-6..
MLA	Shan-li Zhu,et al."Immunogenicity of multi-epitopes gene of major outer membrane protein of Chlamydia trachomatis".Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] 43.3(2009):232-6..